Team:Newcastle/Meetings/12 July 2010

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====General:====
====General:====
*Wiki checkers: Deena and Rachel (after altering/updating a page on the wiki, team members should send the links to the pages to the wiki checkers)
*Wiki checkers: Deena and Rachel (after altering/updating a page on the wiki, team members should send the links to the pages to the wiki checkers)
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==20th/21st July - UK iGEM Gathering at Newcastle==
==20th/21st July - UK iGEM Gathering at Newcastle==

Revision as of 09:17, 13 July 2010

Chair: Da, Minutes: Younus, Comp

Contents

Cloning Strategies

5 parts to a Cloning Strategy:

The cloning strategies for each biobrick should contain the following sections in this order:

  1. Purpose and Justification
  2. Modelling
  3. Construction
  4. Cloning and Integration
  5. Testing and Characterising


Required Cloning Strategies

There are 8 cloning strategies which are required:

  • SR1 - finished!
  • YneA - biobrick nearly coming back - our most important biobrick!
  • LacI - not sure how to test this biobrick
  • 2 for Swarming (sfp and swrA) - cloning strategies nearly finished
  • 2 for Subtilin (immunity and production) - cloning strategies nearly finished
  • RocF (need to use Gibson method) - not started

So only the cloning strategy for SR1 is finished. 7 more to go!


Allocation of team members to cloning strategies:

  • YneA - Phil, Rach, Deena, Younus
  • Swarming (2) - Harsh, Alan, Steve, Da
  • LacI - Phil, Younus, Rachel
  • Subtilin (2) - Harsh, Alan, Steve, Deena
  • RocF - Harsh, Alan, Steve, Da


Wiki

Labbook

Everyone needs to carry out the following protocol for each day in the lab

  1. Aim
  2. Materials and Methods
  3. Procedure/Protocol
  4. Results
  5. Conclusion

Relevant photographs should be added and labelled

General:

  • Wiki checkers: Deena and Rachel (after altering/updating a page on the wiki, team members should send the links to the pages to the wiki checkers)


20th/21st July - UK iGEM Gathering at Newcastle

Presentation:

Introduction

[2 mins] Rach, Deena, Jannetta

  • Cover Page
  • Picture of us standing up
  • Introduction of our iGEM project - using BS to repair concrete

General Outlook

[5 mins] Harsh, Da, Phil

  • Starting with bioreactor
  • Transformation into spores
  • Transportation
  • Spraying on building
  • Germination
  • sensing crack + non-environmental kill switch
  • swarming
  • end of crack signalling = QS
  • Filamentous cells
  • CaCO3
  • Glue
  • Kill Switch

BioBrick Discussion

[8 mins altogether; 2 mins each] Alan, Younus, Steve

  • Swarming
  • Subtillin
  • Filamentous cells - YneA
  • CaCO3 - Urease

(5 mins remaining)

Whats next...

'Save the babies!!!'


Minideadlines

  1. Cloning Strategies
    • YneA
    • One sequence for sfp primers and coding sequence ready (Goksel)
    • Subtilin just immunity


Action Points

  1. Finish wiki updates by Sunday 18th (everyone)
  2. Team page on Wiki:
    • Add Colin Harwood, Colin Daviy, Wendy Smith and Goksel Misrili to list of advisors
    • Deena, Phil and Younus to write about yourself + pictures
Retrieved from "http://2010.igem.org/Team:Newcastle/Meetings/12_July_2010"