Team:Georgia State/Project

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Latest revision as of 00:50, 28 October 2010

2010 Georgia State iGEM project

Purpose #1: Tool Kit

The primary purpose of the Georgia State 2010 team is to standardize parts that will facilitate future use of methylotrophic yeast "Pichia pastoris" as a novel chassis for iGEM purposes. We are titling this assembly of Pichia relevant parts as the “Pichia Tool Kit”, which is intended to be a constantly growing and improving resource. So far parts have included promoters, auxotrophic-mutant complementary proteins, selectivity parts and an antigen for intended production. Below is a diagram illustrating the parts that are currently in progress. Click on each image to go directly to the specific tool and get more information.

[[Image:His4.png|170px|http://partsregistry.org/Part:BBa_K431006 |alt=Alt text|Title text]]

Purpose #2: Vaccine Production

Because of these reasons, P.pastoris has quickly gained popularity for recombinant protein production. The Georgia State 2010 team believes P. pastoris would be an excellent chassis for the iGEM competition. Our goal is to provide a tool box of parts necessary for the genetic manipulation of this organism. Parts will include a plasmid backbone, several parts providing alternative selectivity options and promoter systems. In addition, our tool box will be used to produce a flu virus antigen in P. pastoris as an example of how this system could be used for vaccine production. We hope our contributions will enable future users to maximize the use and further explore the incredible potential P. pastoris has to offer!

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 {{Georgia_State/Header}}
-Over the past month, the team has worked diligently in preparing P. pastoris and Cyanobacteria competency, transforming DNA BioBrick parts, and optimizing growth conditions for future transformations.  In addition, the group convened for the first annual GSU iGEM Boot Camp to work on team building, understanding the basic concepts, and organizing public relations.
-==Production of Herpes Symplex B Virus Antigen in Pichia Pastoris==
-[[Image:Pichia_pastoris.jpg|right]]'''Pichia pastoris''' is a yeast used as an alternative host for protein production in addition to Escherichia coli and Saccharomyces cerevisiae. The use of P. pastoris is advantageous because it can be easily grown, and it can introduce eukaryotic post-translational modifications. In addition, P. pastoris recombinant protein yields are very high (Cereghino and Cregg, 2000).  The team’s goal is to create a part that encodes for Herpes Symplex B antigen and a flagellin part that aids in its immunogenicity.  Together, these parts will be transformed into the yeast for post-translational modification and mass production for vaccine development.
-==Degradation of Toluene using Genetically Engineered Cyanobacteria==
-The recent oil spill in the Gulf of Mexico presents significant pressure to develop new bioremediation technologies. Contamination of sensitive environments can often have devastating ecological and economical repercussions. Monoaromatic compounds such as  Benzene, Toluene, Ethyl benzene, and Xylene (BTEX), are particularly toxic and present in industrial products like crude oil and paint thinners.  Although there are microorganisms capable of degrading BTEX, they are not always optimal for bioremediation in certain environments.  It would be ideal to transform these enzymatic functions into organisms better suited for such conditions.
-We propose to utilize Cyanobacteria as a model organism to host a toluene degradation plasmid and contribute to the bioremediation of marine oil spills. High cell densities of Cyanobacteria are found in many of the world’s oceans, and their unique oxygenic ability is useful in the hypoxic environment of an oil spill. The plasmid will be constructed primarily from parts built from the Pseudomonas putida TOL plasmid. Engineered Cyanobacteria could serve as an effective and environmentally-conscious treatment for oil spill contamination.
+==2010 Georgia State iGEM project==
+'''Purpose #1: Tool Kit''' [[Image:Pichia_tool_box_picture.jpg|right|300px]]
+The primary purpose of the Georgia State 2010 team is to standardize parts that will facilitate future use of methylotrophic yeast "Pichia pastoris" as a novel chassis for iGEM purposes. We are titling this assembly of Pichia relevant parts as the “Pichia Tool Kit”, which is intended to be a constantly growing and improving resource. So far parts have included promoters, auxotrophic-mutant complementary proteins, selectivity parts and an antigen for intended production. Below is a diagram illustrating the parts that are currently in progress. Click on each image to go directly to the specific tool and get more information.
+[[Image:AOX1.png|left|170px]]   [[Image:FLD1.png|170px]]   [[Image:HA antigen.png|170px]]
+[[Image:His4.png|170px]]   [[Image:pGAP.png|170px]]   [[Image:pTEF.png|170px]]
+<code><nowiki>[[Image:His4.png|170px|http://partsregistry.org/Part:BBa_K431006 |alt=Alt text|Title text]]</nowiki></code>
+'''Purpose #2: Vaccine Production'''
+Because of these reasons, ''P.pastoris'' has quickly gained popularity for recombinant protein production. The Georgia State 2010 team believes ''P. pastoris'' would be an excellent chassis for the iGEM competition. Our goal is to provide a tool box of parts necessary for the genetic manipulation of this organism. Parts will include a plasmid backbone, several parts providing alternative selectivity options and promoter systems. In addition, our tool box will be used to produce a flu virus antigen in ''P. pastoris'' as an example of how this system could be used for vaccine production. We hope our contributions will enable future users to maximize the use and further explore the incredible potential ''P. pastoris'' has to offer!
+[[Image:vaccine.jpg|right|700px]]
 {{Georgia_State/Footer}}