Team:Berkeley

From 2010.igem.org

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This is a template page. READ THESE INSTRUCTIONS.
 
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2009.igem.org/Help:Template/Examples">HERE</a>.
 
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
 
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<center> [[Image:ChoaChoa delivery header.png |950px]]</center>
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<center>(Pronounced like "Cocoa")</center>
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Single-celled phagocytic eukaryotes, such as Choanoflagellates, are of great interest to developmental biologists because they may be the last living immediate precursor on the evolutionary tree to animals. These easy-to-culture and robust organisms are also a desirable low-cost eukaryotic chassis for synthetic biology, but there are few to no tools for delivering biomolecules into these organisms. We engineered E. coli to deliver proteins and/or DNA payloads into these bacteria-devouring eukaryotes. Once ingested, our E. coli are programmed to self-lyse and porate the phagosome, releasing their payloads into the cytosol. This delivery mechanism has the potential to deliver payload to any phagocytic organism with a cholesterol-based membrane. As part of our parallel software effort to rework the Clotho plugin environment and API, we made automatic biosafety handling an intrinsic feature of the core. Together, these tools provide a foundation for metazoan synthetic biology and a framework for improving safety in our field.
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|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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|[[Image:Berkeley_logo.png|200px|right|frame]]
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''Tell us more about your project. Give us background. Use this as the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
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|[[Image:Berkeley_team.png|right|frame|Your team picture]]
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|align="center"|[[Team:Berkeley | Team Example]]
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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!align="center"|[[Team:Berkeley|Home]]
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===Sponsors===
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!align="center"|[[Team:Berkeley/Team|Team]]
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Berkeley Official Team Profile]
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!align="center"|[[Team:Berkeley/Project|Project]]
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!align="center"|[[Team:Berkeley/Parts|Parts Submitted to the Registry]]
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<br>
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!align="center"|[[Team:Berkeley/Modeling|Modeling]]
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<a href="http://www.quintarabio.com/">
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!align="center"|[[Team:Berkeley/Notebook|Notebook]]
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<img src="https://static.igem.org/mediawiki/2010/f/ff/Logo_quintara.jpg" width="178" height="37">
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!align="center"|[[Team:Berkeley/Safety|Safety]]
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&nbsp;&nbsp;&nbsp;
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<a href="http://usa.autodesk.com/">
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<img src="https://static.igem.org/mediawiki/2010/c/c8/Logo_autodesk.jpg" width="217" height="37">
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</a>
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&nbsp;&nbsp;&nbsp;
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<a href="http://www.qb3.org/">
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<img src="https://static.igem.org/mediawiki/2010/2/26/Logo_qb3.jpg" width="57" height="37">
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</a>
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&nbsp;&nbsp;&nbsp;
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<a href="http://www.synberc.org/">
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<img src="https://static.igem.org/mediawiki/2010/3/3c/Logo_synberc.jpg" width="141" height="37">
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</a>
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&nbsp;&nbsp;&nbsp;
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<a href="http://www.yaliscafe.com/">
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<img src="https://static.igem.org/mediawiki/2010/e/e1/Yaligray.png" width="37" height="37">
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<br>&nbsp;</br>
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We would like to thank our sponsors, shown above, and our wonderful advisors Chris Anderson, Terry Johnson, Tim Hsiau, and Jin Huh for their guidance and support.

Latest revision as of 23:43, 27 October 2010

Plain header.png




ChoaChoa delivery header.png
(Pronounced like "Cocoa")

Single-celled phagocytic eukaryotes, such as Choanoflagellates, are of great interest to developmental biologists because they may be the last living immediate precursor on the evolutionary tree to animals. These easy-to-culture and robust organisms are also a desirable low-cost eukaryotic chassis for synthetic biology, but there are few to no tools for delivering biomolecules into these organisms. We engineered E. coli to deliver proteins and/or DNA payloads into these bacteria-devouring eukaryotes. Once ingested, our E. coli are programmed to self-lyse and porate the phagosome, releasing their payloads into the cytosol. This delivery mechanism has the potential to deliver payload to any phagocytic organism with a cholesterol-based membrane. As part of our parallel software effort to rework the Clotho plugin environment and API, we made automatic biosafety handling an intrinsic feature of the core. Together, these tools provide a foundation for metazoan synthetic biology and a framework for improving safety in our field.


Sponsors


               
 

We would like to thank our sponsors, shown above, and our wonderful advisors Chris Anderson, Terry Johnson, Tim Hsiau, and Jin Huh for their guidance and support.

Retrieved from "http://2010.igem.org/Team:Berkeley"