Team:EPF Lausanne/Protocols

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Latest revision as of 23:15, 27 October 2010

Lab Protocols

On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.

Adapt pH
Agarose Gel
Agar plates production
Autoclave
Electrophoresis
Growing Asaia
Ligation
Measuring DNA concentration using NanoDrop
Miniprep: use Miniprep Kit
Optical Density (OD)
PCR Roche
PCR iProof
Purification: use Purification Kit
Transformation of competent cells
UV Imaging
Digestion
Western Blot

Media Recipes

Syntax:convention if you want to upload a new protocol
When uploading a file it please rename it in the following way: prot_yourProcedureName.yourExtension.

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-[https://2010.igem.org/Team:EPF_Lausanne home]
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 == Lab Protocols ==
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 *[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave ]
 *[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis]
-* Growing Asaia (Effie)
+*[https://static.igem.org/mediawiki/igem.org/8/82/Prot_growing_asaia.pdf Growing Asaia]
 *[https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation]
 *[https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop]
-* Miniprep --> use Miniprep Kit
+* Miniprep: use Miniprep Kit
 *[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf Optical Density (OD)]
-*[https://static.igem.org/mediawiki/2010/6/6c/Prot_PCR.pdf PCR ]
+*[https://static.igem.org/mediawiki/2010/5/54/Protocol_pcr_roche.pdf PCR Roche ]
-* Purification --> use Purification Kit
+*[https://static.igem.org/mediawiki/2010/6/6a/Prot_pcr_iproof.pdf PCR iProof]
+* Purification: use Purification Kit
 *[https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf Transformation of competent cells]
 *[https://static.igem.org/mediawiki/2010/2/2f/Prot_UV_imaging.pdf UV Imaging]
-*Using the plate reader (Solange)
+*[https://static.igem.org/mediawiki/2010/a/a2/Prot_digestion.pdf Digestion]
+*[https://static.igem.org/mediawiki/2010/6/68/Prot_WesternBlot.pdf Western Blot]
 Media Recipes
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 *[https://static.igem.org/mediawiki/2010/6/6e/Prot_SOC.pdf SOC medium]
+<b>Syntax:</b>convention if you want to upload a new protocol<br\>
+When uploading a file it please rename it in the following way: <i>prot_yourProcedureName.yourExtension</i>.
-<b>Syntax: </b>convention if you want to upload a new protocol  <br\>
+{{EPFL_2010_bottom_wrap}}
- When uploading a file it please rename it in the following way: <i> prot_yourProcedureName.yourExtension</i>.
+</div>