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Team:EPF Lausanne/Protocols
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== Lab Protocols == | == Lab Protocols == | ||
On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much. | On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much. | ||
| - | * | + | *[https://static.igem.org/mediawiki/2010/f/f9/Prot_adapt_pH.pdf Adapt pH] |
| - | *[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel | + | *[https://static.igem.org/mediawiki/2010/6/6f/Prot_agarose_gel.pdf Agarose Gel] |
| - | *[https://static.igem.org/mediawiki/2010/6/63/Prot_agar_plates.pdf Agar plates production | + | *[https://static.igem.org/mediawiki/2010/6/63/Prot_agar_plates.pdf Agar plates production] |
| - | *[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave | + | *[https://static.igem.org/mediawiki/2010/f/f9/Prot_autoclave.pdf Autoclave ] |
*[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis] | *[https://static.igem.org/mediawiki/2010/8/82/Prot_electrophoresis.pdf Electrophoresis] | ||
| - | * Asaia | + | *[https://static.igem.org/mediawiki/igem.org/8/82/Prot_growing_asaia.pdf Growing Asaia] |
| - | + | *[https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation] | |
| - | *[https://static.igem.org/mediawiki/2010/6/6a/Prot_ligation.pdf Ligation | + | |
*[https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop] | *[https://static.igem.org/mediawiki/2010/f/f1/Prot_nanodrop.pdf Measuring DNA concentration using NanoDrop] | ||
| - | *[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf OD | + | * Miniprep: use Miniprep Kit |
| - | *[https://static.igem.org/mediawiki/2010/6/ | + | *[https://static.igem.org/mediawiki/2010/e/ec/Prot_OD.pdf Optical Density (OD)] |
| - | * | + | *[https://static.igem.org/mediawiki/2010/5/54/Protocol_pcr_roche.pdf PCR Roche ] |
| - | *[https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf Transformation of competent cells | + | *[https://static.igem.org/mediawiki/2010/6/6a/Prot_pcr_iproof.pdf PCR iProof] |
| - | * UV | + | * Purification: use Purification Kit |
| - | * | + | *[https://static.igem.org/mediawiki/2010/1/1e/Prot_transformation.pdf Transformation of competent cells] |
| + | *[https://static.igem.org/mediawiki/2010/2/2f/Prot_UV_imaging.pdf UV Imaging] | ||
| + | *[https://static.igem.org/mediawiki/2010/a/a2/Prot_digestion.pdf Digestion] | ||
| + | *[https://static.igem.org/mediawiki/2010/6/68/Prot_WesternBlot.pdf Western Blot] | ||
Media Recipes | Media Recipes | ||
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*[https://static.igem.org/mediawiki/2010/6/6e/Prot_SOC.pdf SOC medium] | *[https://static.igem.org/mediawiki/2010/6/6e/Prot_SOC.pdf SOC medium] | ||
| + | <b>Syntax:</b>convention if you want to upload a new protocol<br\> | ||
| + | When uploading a file it please rename it in the following way: <i>prot_yourProcedureName.yourExtension</i>. | ||
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Latest revision as of 23:15, 27 October 2010
Lab Protocols
On this page we collect summaries of the procedures we have learned in the lab. These protocols are kept in a very simple non-scientific language since they should also be understood quickly by the non-biology students. They are specific to the lab of professor Maerkl where the iGEM team is working this summer. The protocols should enable the new team members in the lab to learn procedures quickly so that we don't have to bother Henrike and the other TA's too much.
- Adapt pH
- Agarose Gel
- Agar plates production
- Autoclave
- Electrophoresis
- Growing Asaia
- Ligation
- Measuring DNA concentration using NanoDrop
- Miniprep: use Miniprep Kit
- Optical Density (OD)
- PCR Roche
- PCR iProof
- Purification: use Purification Kit
- Transformation of competent cells
- UV Imaging
- Digestion
- Western Blot
Media Recipes
Syntax:convention if you want to upload a new protocol
When uploading a file it please rename it in the following way: prot_yourProcedureName.yourExtension.
