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Team:UCSF/Parts
From 2010.igem.org
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| + | <p>During the summer we built the following devices under the BioBrick standard <a href="https://2009.igem.org/Judging/Variance/UCSF">RFC28</a> (see more info <a href="http://dspace.mit.edu/bitstream/handle/1721.1/46721/BBFRFC28.pdf">here</a>), which was developed by the UCSF team in 2009 and was approved by the Registry of Parts. However, this year the Registry does not accept RFC28. Due to time constrains, we could not move all of these parts into pSB1C3 and submit them as we had planned.</p> | ||
<table id="customers"> | <table id="customers"> | ||
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</tr> | </tr> | ||
| - | <tr> | + | <tr class="alt"> |
<td>John</td> | <td>John</td> | ||
<td>antiCD19CAR-DAP10-STOP</td> | <td>antiCD19CAR-DAP10-STOP</td> | ||
<td>Positive Control</td> | <td>Positive Control</td> | ||
</tr> | </tr> | ||
| - | |||
<tr> | <tr> | ||
<td>Hannah</td> | <td>Hannah</td> | ||
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</tr> | </tr> | ||
| - | <tr> | + | <tr class="alt"> |
<td>Lianna</td> | <td>Lianna</td> | ||
<td>antiCD19CAR-mDAP10N87Q-STOP</td> | <td>antiCD19CAR-mDAP10N87Q-STOP</td> | ||
<td>Negative Control</td> | <td>Negative Control</td> | ||
</tr> | </tr> | ||
| - | |||
<tr> | <tr> | ||
<td>Sam</td> | <td>Sam</td> | ||
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</tr> | </tr> | ||
| - | <tr> | + | <tr class="alt"> |
<td>Crystal</td> | <td>Crystal</td> | ||
<td>antiMESOCAR-DAP10-CD3Z</td> | <td>antiMESOCAR-DAP10-CD3Z</td> | ||
<td>Stronger Signaling</td> | <td>Stronger Signaling</td> | ||
</tr> | </tr> | ||
| - | |||
<tr> | <tr> | ||
<td>Crystal</td> | <td>Crystal</td> | ||
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</tr> | </tr> | ||
| - | <tr> | + | <tr class="alt"> |
<td>Crystal</td> | <td>Crystal</td> | ||
<td>antiMESOCAR-DAP10-DAP10</td> | <td>antiMESOCAR-DAP10-DAP10</td> | ||
<td>Stronger Signaling</td> | <td>Stronger Signaling</td> | ||
</tr> | </tr> | ||
| - | |||
<tr> | <tr> | ||
<td>Sam</td> | <td>Sam</td> | ||
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</tr> | </tr> | ||
| - | <tr> | + | <tr class="alt"> |
<td>Sam</td> | <td>Sam</td> | ||
<td>antiCD19CAR-DAP10-Dap10</td> | <td>antiCD19CAR-DAP10-Dap10</td> | ||
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<tr> | <tr> | ||
<td>Crystal</td> | <td>Crystal</td> | ||
| - | <td>antiMESOCAR -LFA1 -DAP10</td> | + | <td>antiMESOCAR-LFA1-DAP10</td> |
<td>Stronger Signaling</td> | <td>Stronger Signaling</td> | ||
</tr> | </tr> | ||
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<td>antiMESOCAR-OX40-CD3z</td> | <td>antiMESOCAR-OX40-CD3z</td> | ||
<td>Stronger Signaling</td> | <td>Stronger Signaling</td> | ||
| - | |||
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>Stronger Signaling</td> | <td>Stronger Signaling</td> | ||
</tr> | </tr> | ||
| - | |||
<tr> | <tr> | ||
<td>Carmen</td> | <td>Carmen</td> | ||
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<td>antiCD19CAR-OX40-CD3z</td> | <td>antiCD19CAR-OX40-CD3z</td> | ||
<td>Stronger Signaling</td> | <td>Stronger Signaling</td> | ||
| - | |||
</tr> | </tr> | ||
<tr> | <tr> | ||
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<td>Stronger Signaling</td> | <td>Stronger Signaling</td> | ||
</tr> | </tr> | ||
| - | |||
<tr> | <tr> | ||
<td>Carmen</td> | <td>Carmen</td> | ||
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<td>Stronger Signaling</td> | <td>Stronger Signaling</td> | ||
</tr> | </tr> | ||
| - | |||
<tr> | <tr> | ||
<td>Lianna</td> | <td>Lianna</td> | ||
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<td>Stronger Signaling</td> | <td>Stronger Signaling</td> | ||
</tr> | </tr> | ||
| - | |||
<tr> | <tr> | ||
<td>Crystal</td> | <td>Crystal</td> | ||
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<td>Stronger Signaling</td> | <td>Stronger Signaling</td> | ||
</tr> | </tr> | ||
| - | |||
<tr> | <tr> | ||
<td>Crystal</td> | <td>Crystal</td> | ||
Revision as of 23:11, 27 October 2010
Parts
<groupparts>iGEM010 UCSF</groupparts>
During the summer we built the following devices under the BioBrick standard RFC28 (see more info here), which was developed by the UCSF team in 2009 and was approved by the Registry of Parts. However, this year the Registry does not accept RFC28. Due to time constrains, we could not move all of these parts into pSB1C3 and submit them as we had planned.
| Designed by | Ligated parts | Function |
| Carmen | Old antiMesoCAR-LFA1-Dap10 | Stronger Signaling |
| John | antiCD19CAR-DAP10-STOP | Positive Control |
| Hannah | antiCD19CAR-mDAP10y85F-STOP | Stronger Signaling |
| Lianna | antiCD19CAR-mDAP10N87Q-STOP | Negative Control |
| Sam | antiMESOCAR-mDAP10N87Q-STOP | Logic Gate Signaling |
| Crystal | antiMESOCAR-DAP10-CD3Z | Stronger Signaling |
| Crystal | antiMESOCAR- LFA1-CD3z | Stronger Signaling |
| Crystal | antiMESOCAR-DAP10-DAP10 | Stronger Signaling |
| Sam | antiCD19CAR-DAP10-CD3z | Stronger Signaling |
| Sam | antiCD19CAR-DAP10-Dap10 | Stronger Signaling |
| Sam | antiCD19CAR-CD3z-CD3z | Stronger Signaling |
| Carmen | antiMESOCAR-CD3Z-STOP | Positive Control |
| Carmen | antiCD19-CD3z-STOP | Stronger Signaling |
| Carmen | antiMESOCAR-KIR3DL2-STOP | Inhibition |
| Carmen | antiCD19CAR-KIR3DL2-STOP | Inhibition |
| Crystal | antiMESOCAR-LFA1-DAP10 | Stronger Signaling |
| Crystal | antiMESOCAR-mDAP10 M88Q-STOP | Logic Gate Signaling |
| Crystal | antiCD19CAR-mDAP10 M88Q-STOP | Logic Gate Signaling |
| Lianna | antiMESOCAR-OX40-Dap10 | Stronger Signaling |
| Lianna | antiMESOCAR-OX40-CD3z | Stronger Signaling |
| Carmen | antiMESOCAR-FcRg-DAP10 | Stronger Signaling |
| Carmen | antiMESOCAR-ICOS-DAP10 | Stronger Signaling |
| Carmen | antiCD19CAR-FcRg-DAP10 | Stronger Signaling |
| Carmen | antiCD19CAR-ICOS-DAP10 | Stronger Signaling |
| Carmen | antiCD19CAR-LFA1-DAP10 | Stronger Signaling |
| Crystal | antiMESOCAR-CD3z-CD3z | Stronger Signaling |
| Lianna | antiMESOCAR-mDAP10Y85F-STOP | Logic Gate Negative Control |
| Connor | antiCD19CAR-KIR3DL1-STOP | Inhibition |
| Connor | antiMESOCAR-KIR3DL1-STOP | Inhibition |
| Crystal | antiMESOCAR-DAP10-STOP | Stronger Signaling |
| Lianna | antiCD19CAR-OX40-DAP10 | Stronger Signaling |
| Lianna | antiCD19CAR-OX40-CD3z | Stronger Signaling |
| Carmen | antiCD19CAR-SLAMF1-CD3z | Stronger Signaling |
| Carmen | antiCD19CAR-SLAMF1-DAP10 | Stronger Signaling |
| Carmen | antiCD19CAR-SLAMF5-DAP10 | Stronger Signaling |
| John | antiMESOCAR-SLAMF1-CD3z | Stronger Signaling |
| John | antiMESOCAR-SLAMF5-DAP10 | Stronger Signaling |
| Lianna | antiCD19CAR-FcRg-STOP | Stronger Signaling |
| Lianna | antiCD19CAR-ICOS-STOP | Stronger Signaling |
| Lianna | antiCD19CAR-CD28-STOP | Stronger Signaling |
| Lianna | antiMESOCAR-FcRg-STOP | Stronger Signaling |
| Lianna | antiMESOCAR-ICOS-STOP | Stronger Signaling |
| Lianna | antiMESOCAR-CD28-STOP | Stronger Signaling |
| Lianna | antiCD19CAR-LFA1-STOP | Stronger Signaling |
| Lianna | antiMESOCAR-LFA1-STOP | Stronger Signaling |
| Crystal | antiCD19CAR-LFA1-CD3z | Stronger Signaling |
| Crystal | antiMESOCAR-OX40-STOP | Stronger Signaling |
| Crystal | antiMESOCAR-SLAMF3-CD3z | Stronger Signaling |
| Crystal | antiMESOCAR- CD3g-CD3z | Stronger Signaling |
| Crystal | antiMESOCAR-Ly49-STOP | Stronger Signaling |
| Crystal | antiMESOCAR-NKG2A-STOP | Negative Feedback |
| Crystal | antiCD19CAR-OX40-STOP | Stronger Signaling |
| Crystal | antiCD19CAR-SLAMF3-CD3z | Stronger Signaling |
| Crystal | antiCD19CAR-CD3g-CD3z | Stronger Signaling |
| Crystal | antiCD19CAR-FcRg-CD3z | Stronger Signaling |
| Min | GZMB-eGFP-CDMPR | Localization to Cytoxic Granules |
| Min | GZMB-eGFP-GILT | Localization to Cytoxic Granules |
| Min | GZMB-eGFP-LAMP | Localization to Cytoxic Granules |
| Min | GZMB-eGFP-LIMP | Localization to Cytoxic Granules |
| Min | GZMB-eGFP-Ymotif | Localization to Cytoxic Granules |
| Min | GZMB-eGFP-STP | Localization to Cytoxic Granules |
| Min | GZMM-eGFP-CDMPR | Localization to Cytoxic Granules |
| Min | GZMM-eGFP-GILT | Localization to Cytoxic Granules |
| Min | GZMM-eGFP-LAMP | Localization to Cytoxic Granules |
| Min | GZMM-eGFP-LIMP | Localization to Cytoxic Granules |
| Min | GZMM-eGFP-Ymotif | Localization to Cytoxic Granules |
| Min | GZMM-eGFP-STP | Localization to Cytoxic Granules |
