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Team:TU Delft/6 July 2010 content
From 2010.igem.org
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===Lab work=== | ===Lab work=== | ||
| + | The DNA from Mr Gene has finally arrived, now we're ready to build some biobricks! | ||
| - | + | We were so excited; we immediately solved the DNA in water and performed a [[Team:TU_Delft/protocols/transformation|transformation]] with 100 ng of the DNA and plated on the LB agar containing the appropriate antibiotic. | |
| + | |||
| + | 1. AlkB2 on 100 μg/mL Ampicillin | ||
| + | 2. rubA3 on 100 μg/mL Ampicillin | ||
| + | 3. rubA4 on 100 μg/mL Ampicillin | ||
| + | 4. rubB on 50 μg/mL Kanamycin | ||
| + | 5. ladA on 100 μg/mL Ampicillin | ||
| + | 6. ADH on 100 μg/mL Ampicillin | ||
| + | 7. ALDH on 50 μg/mL Kanamycin | ||
| + | 8. bbc1 on 100 μg/mL Ampicillin | ||
| + | 9. AlnA on 100 μg/mL Ampicillin | ||
| + | 10. OprG on 100 μg/mL Ampicillin | ||
| + | 11. PalkS1-2 on 100 μg/mL Ampicillin | ||
| + | 12. PalkB on 100 μg/mL Ampicillin | ||
| + | 13. P(CaiF) on 100 μg/mL Ampenicillin | ||
| + | 14. AlkS on 50 μg/mL Kanamycin | ||
| + | 15. PhPFD-α on 100 μg/mL Ampicillin | ||
| + | 16. PhPFD-β on 100 μg/mL Ampicillin | ||
| + | |||
| + | |||
| + | Pieter is testing different condition for the emulsifying Assay. To determine the emulsifying activity we set up a calibration curve with SDS. He prepared the following samples: | ||
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Revision as of 19:08, 12 July 2010
Lab work
The DNA from Mr Gene has finally arrived, now we're ready to build some biobricks!
We were so excited; we immediately solved the DNA in water and performed a transformation with 100 ng of the DNA and plated on the LB agar containing the appropriate antibiotic.
1. AlkB2 on 100 μg/mL Ampicillin 2. rubA3 on 100 μg/mL Ampicillin 3. rubA4 on 100 μg/mL Ampicillin 4. rubB on 50 μg/mL Kanamycin 5. ladA on 100 μg/mL Ampicillin 6. ADH on 100 μg/mL Ampicillin 7. ALDH on 50 μg/mL Kanamycin 8. bbc1 on 100 μg/mL Ampicillin 9. AlnA on 100 μg/mL Ampicillin 10. OprG on 100 μg/mL Ampicillin 11. PalkS1-2 on 100 μg/mL Ampicillin 12. PalkB on 100 μg/mL Ampicillin 13. P(CaiF) on 100 μg/mL Ampenicillin 14. AlkS on 50 μg/mL Kanamycin 15. PhPFD-α on 100 μg/mL Ampicillin 16. PhPFD-β on 100 μg/mL Ampicillin
Pieter is testing different condition for the emulsifying Assay. To determine the emulsifying activity we set up a calibration curve with SDS. He prepared the following samples:
| Hexane (mL) | Tris Buffer pH 8 (mL) | 10% SDS (mL) | |
| B | 1 | 1.1 | 0 |
| 100 | 1 | 1 | 0.1 |
| 50 | 1 | 1.05 | 0.05 |
| 25 | 1 | 1.075 | 0.025 |
| 10 | 1 | 1.09 | 0.01 |
| 5 | 1 | 1.095 | 0.005 |
Kampioenen!!!!
Today the Dutch team will play the semi-final of the World Cup! Hear us cheer! Stop sound
