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Team:Harvard/vectors
From 2010.igem.org
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<img src="https://static.igem.org/mediawiki/2010/d/d1/Harvard2010vectorheading.jpg" width="300px"><br /><br /> | <img src="https://static.igem.org/mediawiki/2010/d/d1/Harvard2010vectorheading.jpg" width="300px"><br /><br /> | ||
<a class="sidebarlinks" href="#" style="color:white">abstract</a> | <a class="sidebarlinks" href="#" style="color:white">abstract</a> | ||
| - | <a class="sidebarlinks" href="Team:Harvard/vectors/notebook" style="color:white">lab notebook</a> | + | <a class="sidebarlinks" href="http:/2010.igem.org/Team:Harvard/vectors/notebook" style="color:white">lab notebook</a> |
| - | <a class="sidebarlinks" href="Team:Harvard/vectors/agro" style="color:white">intro to agrobacteria</a> | + | <a class="sidebarlinks" href="http:/2010.igem.org/Team:Harvard/vectors/agro" style="color:white">intro to agrobacteria</a> |
| - | <a class="sidebarlinks" href="Team:Harvard/vectors/arabidopsis" style="color:white">intro to arabidopsis</a> | + | <a class="sidebarlinks" href="http:/2010.igem.org/Team:Harvard/vectors/arabidopsis" style="color:white">intro to arabidopsis</a> |
| - | <a class="sidebarlinks" href="Team:Harvard/vectors/vectors" style="color:white">meet the vectors</a> | + | <a class="sidebarlinks" href="http:/2010.igem.org/Team:Harvard/vectors/vectors" style="color:white">meet the vectors</a> |
| - | <a class="sidebarlinks" href="Team:Harvard/vectors/parts" style="color:white">parts & primers</a> | + | <a class="sidebarlinks" href="http:/2010.igem.org/Team:Harvard/vectors/parts" style="color:white">parts & primers</a> |
| - | <a class="sidebarlinks" href="Team:Harvard/vectors/results" style="color:white">results</a> | + | <a class="sidebarlinks" href="http:/2010.igem.org/Team:Harvard/vectors/results" style="color:white">results</a> |
| - | <a class="sidebarlinks" href="Team:Harvard/vectors/references" style="color:white">references</a> | + | <a class="sidebarlinks" href="http:/2010.igem.org/Team:Harvard/vectors/references" style="color:white">references</a> |
</div> | </div> | ||
Revision as of 18:43, 12 July 2010
abstract lab notebook intro to agrobacteria intro to arabidopsis meet the vectors parts & primers results references
abstract
Our goal is the creation of an "iGarden" with the biobrick standard and in order for such a concept to work, we must get our constructs into plants. We used agrobacterium-mediated transformation, first in arabidopsis, then in strawberries.
To do so, we created a set of vectors compatible with the biobrick standard. To express our constucts - such as flavor elements, allergen knockdown, or fence mechanisms, we cloned the constucts into the agrobacterium vectors with E. coli as the chassis, then transformed into agrobacteria via electroporation, then innoculated our plants with the contruct containing agrobacteria.
