Team:ESBS-Strasbourg/Results/Characterization
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consisting of E. coli expressing the GFP. On the right is the culture of <br> | consisting of E. coli expressing the GFP. On the right is the culture of <br> | ||
E. coli expressing PIF6-linker-GFP. | E. coli expressing PIF6-linker-GFP. | ||
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The table below shows the tests made on the previews tubes. Cells were resuspended and sonicated and the lysate was analyzed with a fluorimetre (absorption wavelenght= 490nm, emission wavelenght= 510nm). The test was done 3 times to give an average measurement. We then calculated the specific fluorescence according to the OD600, the results are also shown in the table.<br> | The table below shows the tests made on the previews tubes. Cells were resuspended and sonicated and the lysate was analyzed with a fluorimetre (absorption wavelenght= 490nm, emission wavelenght= 510nm). The test was done 3 times to give an average measurement. We then calculated the specific fluorescence according to the OD600, the results are also shown in the table.<br> | ||
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Revision as of 22:28, 27 October 2010
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