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Team:USTC/Notebook
From 2010.igem.org
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== Preliminary work == | == Preliminary work == | ||
| - | *From Feb.4th to Feb.13th, a collective training is held targeting to the practice of basic experiment. Setting GFP protein expression system as an example, we learn from the program how to construct standard components and then an expression system. It`s the time we get to know each other and become a team as a whole. | + | *From Feb.4th to Feb.13th, a [[Team:USTC/Gallery]] collective training is held targeting to the practice of basic experiment. Setting GFP protein expression system as an example, we learn from the program how to construct standard components and then an expression system. It`s the time we get to know each other and become a team as a whole. |
**The contents of the training are listed as followed: | **The contents of the training are listed as followed: | ||
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***3. plasmid extraction | ***3. plasmid extraction | ||
***4. PCR | ***4. PCR | ||
| - | ***5. | + | ***5. DNA Electrophoresis |
| - | ***6. | + | ***6. Double Digest |
***7. reclaim and appraise of the product of enzyme cutting | ***7. reclaim and appraise of the product of enzyme cutting | ||
| - | ***8. | + | ***8. Ligation |
***9. preparation of competent cell | ***9. preparation of competent cell | ||
***10. genomic screening of masculine clone | ***10. genomic screening of masculine clone | ||
***11. DNA sequencing | ***11. DNA sequencing | ||
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Latest revision as of 21:49, 27 October 2010
Preliminary work
- From Feb.4th to Feb.13th, a Team:USTC/Gallery collective training is held targeting to the practice of basic experiment. Setting GFP protein expression system as an example, we learn from the program how to construct standard components and then an expression system. It`s the time we get to know each other and become a team as a whole.
- The contents of the training are listed as followed:
- 1. outlines
- 2. E.coli culture (Medium preparation, Inoculation, Aseptic operation)
- 3. plasmid extraction
- 4. PCR
- 5. DNA Electrophoresis
- 6. Double Digest
- 7. reclaim and appraise of the product of enzyme cutting
- 8. Ligation
- 9. preparation of competent cell
- 10. genomic screening of masculine clone
- 11. DNA sequencing
- The contents of the training are listed as followed:
Lab Notebook
Meetings are set every Saturday since Feb.15th, then three more discussions (Mon, Wes, Fri) are added when we start to determine our project since Mar 21th.
- 2010.4.10 – 2010.5.14 reports of the former iGEM works
- 2010.5.21 – 2010.6.11 broach of candidate projects
- cell density control
- Reporter and imaging
- DNA writing and reading in the cell
- Biodegradation of plastics
- 2010.6.12 – 2010.6.30 further research by small groups on the candidate projects
- sketch map of our final project host-cell free reaction chamber was set up
All the reports information, slides and audio records of our meetings are arranged here.
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