Team:Slovenia/METHODS and PARTS/notebook/protein
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+ | <a href="/Team:Slovenia/METHODS_and_PARTS/notebook"><span id="stopnja3" >split/FRET</span></a> | ||
+ | <a href="/Team:Slovenia/METHODS_and_PARTS/notebook/oscil"><span id="stopnja3">oscillator</span></a> | ||
+ | <a href="/Team:Slovenia/METHODS_and_PARTS/notebook/viola"><span id="stopnja3" margin-left:15px;">violacein</span></a> | ||
+ | <a href="/Team:Slovenia/METHODS_and_PARTS/notebook/carot"><span id="stopnja3" >carotenoids</span></a> | ||
+ | <a href="/Team:Slovenia/METHODS_and_PARTS/notebook/protein"><span id="stopnja3a">proteins</span></a> | ||
+ | <a href="/Team:Slovenia/METHODS_and_PARTS/notebook/znf"><span style="font-size:15px; | ||
+ | width:167px;" id="stopnja3">zinc-finger binding test</span></a> | ||
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Latest revision as of 21:39, 27 October 2010
notebook - proteins
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8/23/2010 – 8/29/2010
Production of protein: cCFP_link_HivC (5 hours, 37°C, 160 RPM). Protein accumulated mainly in inclusion bodies Cell lysates Protein analyzed by SDS-PAGE and western blot using antibodies agains His-Tag. Further protein purification of protein in solubilized inclusion bodies was attempted by Ni-NTA column, and elution by increasing concentration of imidazole (25mM, 50mM, 100mM, 250mM, 500mM). After concentration using Centricon with the cutoff, the protein was re-analyzed by SDS page and Western blot.