Team:Georgia State/Notebook

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(Prototype team page)
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This is a template page. READ THESE INSTRUCTIONS.
 
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
 
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
 
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{|align="justify"
 
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
 
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|[[Image:Georgia_State_logo.png|200px|right|frame]]
 
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
 
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==Notebook==
==Notebook==
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===July 8, 2010===
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''Angie, Kendra, Melissa, Nishedh, Alykhan''
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*Diluted pichia cells in a volume of 50mL to a OD<sub>600</sub> = .186+.201
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*Transform pars 12E and 12M into E. coli
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===July 7, 2010===
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''Joe, Kendra, Angie''
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*Replated colonies from 9A RFP plate (3 plates).
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*Colonies on broth 100 µL for 9A but no fluorescence observed
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*9A survivor colonies replated onto 10 µg/mL ampicillin plates and incubated at 30&deg;C
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*Mother plate in fridge
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*Made 1000mL YPD
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*Growing P. anomala in YPD broth for competent cells protocol
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===July 6, 2010===
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''Dan''
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*Replated E. coli culture
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''Alykhan''
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*Transformation 9A, 8I (digested) & GFP.
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===July 2, 2010===
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''Alykhan and Virginia''
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*DNA purification and ligation
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*Replated E. coli culture
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You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
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===July 1, 2010===
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''Joe''
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*Plated transformed cells containing either GFP (control) or iGEM part onto LB plates with 10µg/mL ampicillin (2 plates each, 10mL or 100mL)
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*Also plated ≈100mL (remaining amount) of cells onto LB plates not containing ampicillin.
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*Spread plates with hockey stick and placed in 37&deg;C at 7:35.

Revision as of 15:14, 12 July 2010


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety


Contents

Notebook

July 8, 2010

Angie, Kendra, Melissa, Nishedh, Alykhan

  • Diluted pichia cells in a volume of 50mL to a OD600 = .186+.201
  • Transform pars 12E and 12M into E. coli

July 7, 2010

Joe, Kendra, Angie

  • Replated colonies from 9A RFP plate (3 plates).
  • Colonies on broth 100 µL for 9A but no fluorescence observed
  • 9A survivor colonies replated onto 10 µg/mL ampicillin plates and incubated at 30°C
  • Mother plate in fridge
  • Made 1000mL YPD
  • Growing P. anomala in YPD broth for competent cells protocol

July 6, 2010

Dan

  • Replated E. coli culture

Alykhan

  • Transformation 9A, 8I (digested) & GFP.

July 2, 2010

Alykhan and Virginia

  • DNA purification and ligation
  • Replated E. coli culture

July 1, 2010

Joe

  • Plated transformed cells containing either GFP (control) or iGEM part onto LB plates with 10µg/mL ampicillin (2 plates each, 10mL or 100mL)
  • Also plated ≈100mL (remaining amount) of cells onto LB plates not containing ampicillin.
  • Spread plates with hockey stick and placed in 37°C at 7:35.
Retrieved from "http://2010.igem.org/Team:Georgia_State/Notebook"