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Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test14settembre
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| - | <html><p align="center"><font size="5"><b> | + | <html><p align="center"><font size="5"><b>SEPTEMBER, 14TH</b></font></p></html><hr><br> |
==PLATE== | ==PLATE== | ||
<br> | <br> | ||
| - | + | [[Image:UNIPV_Pavia_piastra14sett.png| 620px | center ]] | |
<br> | <br> | ||
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==EXPERIMENT DESCRIPTION== | ==EXPERIMENT DESCRIPTION== | ||
| - | == | + | |
| + | ===Motivation=== | ||
| + | This experiment was performed in LB to check bacterial growth and RFP rate synthesis in order to evaluate the R.P.U. ([[Team:UNIPV-Pavia/Parts/Characterization#Data analysis for RPU evaluation|see Protocol]]) of the promoters from Anderson's collection in low copy number plasmids. | ||
| + | |||
| + | ===Methods=== | ||
| + | Accordign to [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for constitutive promoters (R.P.U. evaluation) - Protocol #2|Protocol 2]] the experiment was performed in LB on the following parts: | ||
| + | |||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23100</partinfo> (wiki name: BBa_J23100_4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23101</partinfo> (wiki name: BBa_J23101_4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23105</partinfo> (wiki name: BBa_J23105_4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23106</partinfo> (wiki name: BBa_J23106_4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23110</partinfo> (wiki name: BBa_J23110_4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23114</partinfo> (wiki name: BBa_J23114_4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23116</partinfo> (wiki name: BBa_J23116_4C5) | ||
| + | * <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23118</partinfo> (wiki name: BBa_J23118_4C5) | ||
| + | * <partinfo>BBa_B0031</partinfo> (negative control) | ||
| + | |||
| + | ==RESULTS== | ||
| + | Unfortunately it was not possbile to read properly the fluorescence that resulted too weak. | ||
| + | |||
| + | [[Image:UNIPV_Pavia_fluoLC.png| 620px | center ]] | ||
<!-- table previous next test --> | <!-- table previous next test --> | ||
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<table border="0" width="100%" class="menu"> | <table border="0" width="100%" class="menu"> | ||
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| - | <td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/ | + | <td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test10settembre_bis|Previous Test]]</td> |
| - | <td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/ | + | <td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test16settembre| Next Test]]</td> |
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| - | + | {{UNIPV-Pavia/Menu_measurments}} | |
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Latest revision as of 21:26, 27 October 2010
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