Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test14settembre

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<html><p align="center"><font size="5"><b>18° TEST</b></font><br><br><font size="4">[SEPTEMBER, 14TH]</font></p></html><hr><br>
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<html><p align="center"><font size="5"><b>SEPTEMBER, 14TH</b></font></p></html><hr><br>
==PLATE==
==PLATE==
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<!--[[Image:UNIPV_Pavia_piastra27agosto.png| 620px | center ]]-->
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[[Image:UNIPV_Pavia_piastra14sett.png| 620px | center ]]
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==EXPERIMENT DESCRIPTION==
==EXPERIMENT DESCRIPTION==
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==RESULTS (?)==
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===Motivation===
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This experiment was performed in LB to check bacterial growth and RFP rate synthesis in order to evaluate the R.P.U. ([[Team:UNIPV-Pavia/Parts/Characterization#Data analysis for RPU evaluation|see Protocol]]) of the promoters from Anderson's collection in low copy number plasmids.
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===Methods===
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Accordign to [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for constitutive promoters (R.P.U. evaluation) - Protocol #2|Protocol 2]] the experiment was performed in LB on the following parts:
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23100</partinfo> (wiki name: BBa_J23100_4C5)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23101</partinfo> (wiki name: BBa_J23101_4C5)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23105</partinfo> (wiki name: BBa_J23105_4C5)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23106</partinfo> (wiki name: BBa_J23106_4C5)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23110</partinfo> (wiki name: BBa_J23110_4C5)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23114</partinfo> (wiki name: BBa_J23114_4C5)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23116</partinfo> (wiki name: BBa_J23116_4C5)
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* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23118</partinfo> (wiki name: BBa_J23118_4C5)
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* <partinfo>BBa_B0031</partinfo> (negative control)
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==RESULTS==
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Unfortunately it was not possbile to read properly the fluorescence that resulted too weak.
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[[Image:UNIPV_Pavia_fluoLC.png| 620px | center ]]
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<td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test17|Previous Test]]</td>
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<td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test10settembre_bis|Previous Test]]</td>
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<td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test19| Next Test]]</td>
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<td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test16settembre| Next Test]]</td>
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{{UNIPV-Pavia/Menu_measurments}}
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[[Team:UNIPV-Pavia/Material_Methods/Measurements|MEASUREMENTS]]
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[[Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan|Tecan Test]]
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Latest revision as of 21:26, 27 October 2010
Home
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SEPTEMBER, 14TH


PLATE


UNIPV Pavia piastra14sett.png


EXPERIMENT DESCRIPTION

Motivation

This experiment was performed in LB to check bacterial growth and RFP rate synthesis in order to evaluate the R.P.U. (see Protocol) of the promoters from Anderson's collection in low copy number plasmids.

Methods

Accordign to Protocol 2 the experiment was performed in LB on the following parts:

  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23100</partinfo> (wiki name: BBa_J23100_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23101</partinfo> (wiki name: BBa_J23101_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23105</partinfo> (wiki name: BBa_J23105_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23106</partinfo> (wiki name: BBa_J23106_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23110</partinfo> (wiki name: BBa_J23110_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23114</partinfo> (wiki name: BBa_J23114_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23116</partinfo> (wiki name: BBa_J23116_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23118</partinfo> (wiki name: BBa_J23118_4C5)
  • <partinfo>BBa_B0031</partinfo> (negative control)

RESULTS

Unfortunately it was not possbile to read properly the fluorescence that resulted too weak.

UNIPV Pavia fluoLC.png



Previous Test Next Test

Tecan Test

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