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| | __NOTOC__ | | __NOTOC__ |
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| - | == DIY-GEM: a path towards low cost high throughput gene synthesis. ==
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| - | Synthetic biology research requires more cost effective approaches toward reagents and hardware accessibility. We are developing low-cost alternatives to existing hardware and enzymes in an attempt to expand participation in biological research and development. Our project expands the accessibility of Taq Polymerase by engineering it in a form compatible with BioBrick assembly. This allows use of the over-expressed enzyme from a crude bacterial extract in a PCR reaction at a fraction of the cost of highly purified commercial enzyme. In addition, we have developed inexpensive and easily assembled lab equipment such as a gel electrophoresis apparatus and a PCR thermal cycler. Enabling researchers to synthesize their own enzymes and having access to inexpensive tools will allow for increased participation among the DIY-bio community, stretch increasingly scarce educational funds, and allow rapid scale up of large scale gene synthesis projects.<br>
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| | |[[Image:Baltimore_US_team.png|400px]] | | |[[Image:Baltimore_US_team.png|400px]] |
| | |} | | |} |
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| | <!--- The Mission, Experiments ---> | | <!--- The Mission, Experiments ---> |
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| | == '''Who we are''' == | | == '''Who we are''' == |
| | {|border = "0" | | {|border = "0" |
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| | |rowspan="3"| | | |rowspan="3"| |
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| | '''Advisors:''' | | '''Advisors:''' |
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| - | *''' Lab Manager/Advisor''': Thomas Burkett | + | *''' Lab Manager/Advisor''': Thomas Burkett |
| - | *'''Advisor 1''': Lisa Scheifele | + | *'''Advisor ''': Lisa Scheifele |
| - | *'''Advisor 2''': T Elizabeth Goode | + | *'''Advisor ''': T Elizabeth Goode |
| - | | + | <br> |
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| | '''Undergrads:''' | | '''Undergrads:''' |
| | + | *Ryan Ogle, LMT |
| | + | *Patrick O'Neill |
| | + | *Robert Buck |
| | + | *Miles Pekala |
| | + | *Gary Cygiel |
| | + | *Steven Stowell |
| | + | *Duke Kessler |
| | + | *Bernadette Gallagher |
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| - | *'''Student 1''': Ryan Ogle, LMT
| + | |<gallery> |
| - | *'''Student 2''': Patrick O'Neil
| + | image:Ryan.JPG|'''Ryan Ogle, LMT''' |
| - | *'''Student 3''': Robert Buck
| + | Image:Patrickmugshot.jpg|'''Patrick O'Neill''' |
| - | *'''Student 4''': Miles Pekala
| + | Image:DIYRobert.JPG|'''Robert Buck''' |
| - | *'''Student 5''': Gary Cygiel
| + | Image:DIYMiles.JPG|'''Miles Pekala''' |
| - | *'''Student 6''': Steven Stowell
| + | Image:Bernadette_.JPG|<br>'''Bernadette Gallagher''' |
| - | *'''Student 7''': Duke Kessler
| + | <br> |
| - | *'''Student 8''': Bernadette Gallagher
| + | Image:Burkett.jpg| '''Tom Burkett - Lab Manager/Advisor''' |
| - | | + | Image:Scheifele.jpg|'''Lisa Scheifele - Advisor''' |
| - | | + | Image:Goode.jpg| '''Liz Goode - Advisor''' |
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| - | <gallery> | + | |
| - | Image:Ryan.JPG|''Go Team Venture!'' <br><br>'''Ryan Ogle, LMT'''
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| - | Image:Patrickmugshot.jpg|<br><br>'''Patrick O'Neill''' | + | |
| - | Image:Baltimore_US_Team_member_3.png|(Robert's mugshot here) <br><br>'''Robert Buck''' | + | |
| - | Image:Baltimore_US_Team_member_4.png|(Miles' mugshot here) <br><br>'''Miles Pekala''' | + | |
| - | Image:Baltimore_US_Team_member_5.png|(Gary's mugshot here) <br><br>'''Gary Cygiel''' | + | |
| - | Image:Baltimore_US_Team_member_6.png|(Steve's mugshot here) <br><br>'''Steven Stowell'''
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| - | Image:Baltimore_US_Team_member_7.png|(Duke's mugshot here) <br><br>'''Duke Kessler''' | + | |
| - | Image:Bernadette_.JPG|(Bernadette's mugshot here) <br>'''Bernadette Gallagher'''
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| - | Image:Baltimore_US_Team_member_9.png|(Tom's mugshot here) <br><br>'''Tom Burkett - Lab Manager/Advisor & Contact Person'''
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| - | Image:Baltimore_US_Team__member_10.png|(Lisa's mugshot here) <br><br>'''Lisa Scheifele - Advisor''' | + | |
| - | Image:Baltimore_US_Team_member_11.png|(Liz's mugshot here )<br><br> '''Liz Goode - Advisor''' | + | |
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| | </gallery> | | </gallery> |
| | |} | | |} |
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| | == '''What we did''' == | | == '''What we did''' == |
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| - | [[Image:DIYPatrick1.JPG|300px]]
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| - | [[Image:DIYMiles.JPG|300px]]
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| - | [[Image:DIYRobert.JPG|300px]]
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| | == '''Where we're from''' == | | == '''Where we're from''' == |
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