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| - | {{EPFL_2010}} | + | {{EPFL_2010_2}} |
| | <html> | | <html> |
| - | <img src="https://static.igem.org/mediawiki/2010/2/2a/EPFL_title_note.png" style="position: relative; top: -87px; left:-5px;"/>
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| - | </html>
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| - | <html> | + | <div style="position: absolute; left: 0px; top: 60px; height: 140px; width: 975px;"> |
| - | <head> | + | <img alt="" src="https://static.igem.org/mediawiki/2010/0/0f/EPFL_Title_2_Notebook.png"/></div> |
| | | | |
| - | <style type="text/css"> | + | <div style="position: absolute; left: 435px; top: 11px; height: 85px; width: 125px;"> |
| | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook"> |
| | + | <img alt="" height="80" src="https://static.igem.org/mediawiki/2010/b/b9/EPFL_Menu_2_Notebook.png" width="125" class="auto-style1" /></a></div> |
| | | | |
| - | .toc {
| + | </html> |
| - | display:none;
| + | |
| - | }
| + | |
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| - | .EPFL_content {
| + | {{EPFL_sub_menu_notebook}} |
| - | margin-left:0px;
| + | |
| - | }
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| - | .style1 {
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| - | .style2 {
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| - | background-color: #FFA8B7;
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| - | .style4 {
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| - | text-align: center;
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| - | font-family: "Lucida Sans Unicode";
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| - | background-color: #FF5371;
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| - | </style>
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| - | </head>
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| - | </html>
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| | | | |
| | | | |
| | <div CLASS="EPFL_content"> | | <div CLASS="EPFL_content"> |
| - |
| |
| - | = How to use the Notebook =
| |
| - | This is a NOTEbook and not a LABbook. Here you can describe what you have done during your exciting day. If you want to have more details you can look in the IRL Labbook. Use ctrl+F to find something ;)
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| - |
| |
| - | == template of the day ==
| |
| - | <html> <table cellspacing="15" > <tr> <td> </html>
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| - | For each new day in the lab you must create a new date in the notebook. To do so, copy-paste the following template:
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| - |
| |
| - | <pre>
| |
| - | = 02-08-2010 =
| |
| - | {| cellspacing="15"
| |
| - | | style="width: 33.33%" valign="top" |
| |
| - | == Other ==
| |
| - | It rain a lot today
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| - |
| |
| - | | style="width: 33.33%" valign="top" |
| |
| - | == Asaia ==
| |
| - | Today I speak with an asaia and she answer to me ^^
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| - |
| |
| - | | style="width: 33.33%" valign="top" |
| |
| - | == Drosophile ==
| |
| - |
| |
| - | |}
| |
| - | </pre>
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| - |
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| - | <html> </td><td> </html>
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| - |
| |
| - | <u>Here are some useful explanations : </u>
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| - |
| |
| - | 1. The date format is important!
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| - |
| |
| - | 2. Just after the date, put the following two lines :
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| - | <pre>
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| - | {| cellspacing="15"
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| - | | style="width: 33.33%" valign="top" |
| |
| - | </pre>
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| - | 3. Beetween each subtitle, put this line :
| |
| - | <pre>
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| - | | style="width: 33.33%" valign="top" |
| |
| - | </pre>
| |
| - | 4. To end the day just put :
| |
| - | <pre>
| |
| - | |}
| |
| - | </pre>
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| - | 5. Of course the text "width:33.33%" must be adapted on how many subtitle you have (for instance 4 subtitles = 25%)
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| - |
| |
| - | <html></td></tr></table></html>
| |
| - |
| |
| - | = Calendar =
| |
| | | | |
| | <html> | | <html> |
| - | <table style="width: 100%">
| |
| - | <tr class="style4">
| |
| - | <td>Week</td>
| |
| - | <td>Monday</td>
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| - | <td>Tuesday</td>
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| - | <td>Wednesday</td>
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| - | <td>Thursday</td>
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| - | <td>Friday</td>
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| - | <td>Saturday</td>
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| - | <td>Sunday</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">28</td>
| |
| - | <td class="style2">
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| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#12-07-2010">
| |
| - | 12-07-2010</a></td>
| |
| - | <td class="style2">
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| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#13-07-2010">
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| - | 13-07-2010</a></td>
| |
| - | <td class="style2">
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| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#14-07-2010">
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| - | 14-07-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#15-07-2010">
| |
| - | 15-07-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#16-07-2010">
| |
| - | 16-07-2010</a></td>
| |
| - | <td class="style2">
| |
| | | | |
| - | 17-07-2010</td>
| + | <div style="position: absolute; left: 10px; top: 50px; height: 200px; width: 170px;"> |
| - | <td class="style2">
| + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week1"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/b/bd/EPFL_Week01.png"/></a></div> |
| | | | |
| - | 18-07-2010</td>
| + | <div style="position: absolute; left: 220px; top: 60px; height: 200px; width: 170px;"> |
| - | </tr>
| + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook /week2"> |
| - | <tr>
| + | <img alt="" src="https://static.igem.org/mediawiki/2010/3/3d/EPFL_Week02.png"/></a></div> |
| - | <td class="style3">29</td>
| + | |
| - | <td class="style2">
| + | |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#19-07-2010">
| + | |
| - | 19-07-2010</a></td>
| + | |
| - | <td class="style2">
| + | |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#20-07-2010">
| + | |
| - | 20-07-2010</a></td>
| + | |
| - | <td class="style2">
| + | |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook #21-07-2010"> | + | |
| - | 21-07-2010</a></td>
| + | |
| - | <td class="style2">
| + | |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#22-07-2010">
| + | |
| - | 22-07-2010</a></td>
| + | |
| - | <td class="style2">
| + | |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#23-07-2010">
| + | |
| - | 23-07-2010</a></td>
| + | |
| - | <td class="style2">
| + | |
| | | | |
| - | 24-07-2010</td>
| + | <div style="position: absolute; left: 430px; top: 45px; height: 200px; width: 170px;"> |
| - | <td class="style2">
| + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week3"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/c/c9/EPFL_Week03.png"/></a></div> |
| | | | |
| - | 25-07-2010</td>
| + | <div style="position: absolute; left: 640px; top: 55px; height: 200px; width: 170px;"> |
| - | </tr>
| + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook /week4"> |
| - | <tr>
| + | <img alt="" src="https://static.igem.org/mediawiki/2010/f/fc/EPFL_Week04.png"/></a></div> |
| - | <td class="style3">30</td>
| + | |
| - | <td class="style2">
| + | |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#26-07-2010">
| + | |
| - | 26-07-2010</a></td>
| + | |
| - | <td class="style2">
| + | |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#27-07-2010">
| + | |
| - | 27-07-2010</a></td>
| + | |
| - | <td class="style2">
| + | |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook #28-07-2010"> | + | |
| - | 28-07-2010</a></td>
| + | |
| - | <td class="style2">
| + | |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#29-07-2010">
| + | |
| - | 29-07-2010</a></td>
| + | |
| - | <td class="style2">
| + | |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#30-07-2010">
| + | |
| - | 30-07-2010</a></td>
| + | |
| - | <td class="style2">
| + | |
| | | | |
| - | 31-07-2010</td>
| |
| - | <td class="style2">
| |
| | | | |
| - | 01-08-2010</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">31</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#02-08-2010">
| |
| - | 02-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#03-08-2010">
| |
| - | 03-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#04-08-2010">
| |
| - | 04-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#05-08-2010">
| |
| - | 05-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#06-08-2010">
| |
| - | 06-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | 07-08-2010</td>
| |
| - | <td class="style2">
| |
| - | 08-08-2010</td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">32</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#09-08-2010">
| |
| - | 09-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#10-08-2010">
| |
| - | 10-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#11-08-2010">
| |
| - | 11-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#12-08-2010">
| |
| - | 12-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#13-08-2010">
| |
| - | 13-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#14-08-2010">
| |
| - | 14-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#15-08-2010">
| |
| - | 15-08-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">33</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#16-08-2010">
| |
| - | 16-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#17-08-2010">
| |
| - | 17-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#18-08-2010">
| |
| - | 18-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#19-08-2010">
| |
| - | 19-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#20-08-2010">
| |
| - | 20-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#21-08-2010">
| |
| - | 21-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#22-08-2010">
| |
| - | 22-08-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">34</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#23-08-2010">
| |
| - | 23-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#24-08-2010">
| |
| - | 24-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#25-08-2010">
| |
| - | 25-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#26-08-2010">
| |
| - | 26-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#27-08-2010">
| |
| - | 27-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#28-08-2010">
| |
| - | 28-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#29-08-2010">
| |
| - | 29-08-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">35</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#30-08-2010">
| |
| - | 30-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#31-08-2010">
| |
| - | 31-08-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#01-09-2010">
| |
| - | 01-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#02-09-2010">
| |
| - | 02-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#03-09-2010">
| |
| - | 03-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#04-09-2010">
| |
| - | 04-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#05-09-2010">
| |
| - | 05-09-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">36</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#06-09-2010">
| |
| - | 06-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#07-09-2010">
| |
| - | 07-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#08-09-2010">
| |
| - | 08-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#09-09-2010">
| |
| - | 09-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#10-09-2010">
| |
| - | 10-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#11-09-2010">
| |
| - | 11-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#12-09-2010">
| |
| - | 12-09-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">37</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#13-09-2010">
| |
| - | 13-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#14-09-2010">
| |
| - | 14-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#15-09-2010">
| |
| - | 15-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#16-09-2010">
| |
| - | 16-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#17-09-2010">
| |
| - | 17-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#18-09-2010">
| |
| - | 18-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#19-09-2010">
| |
| - | 19-09-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">38</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#20-09-2010">
| |
| - | 20-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#21-09-2010">
| |
| - | 21-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#22-09-2010">
| |
| - | 22-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#23-09-2010">
| |
| - | 23-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#24-09-2010">
| |
| - | 24-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#25-09-2010">
| |
| - | 25-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#26-09-2010">
| |
| - | 26-09-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">39</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#27-09-2010">
| |
| - | 27-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#28-09-2010">
| |
| - | 28-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#29-09-2010">
| |
| - | 29-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#30-09-2010">
| |
| - | 30-09-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#01-10-2010">
| |
| - | 01-10-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#02-10-2010">
| |
| - | 02-10-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#03-10-2010">
| |
| - | 03-10-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">40</td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#04-10-2010">
| |
| - | 04-10-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#05-10-2010">
| |
| - | 05-10-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#06-10-2010">
| |
| - | 06-10-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#07-10-2010">
| |
| - | 07-10-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#08-10-2010">
| |
| - | 08-10-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#09-10-2010">
| |
| - | 09-10-2010</a></td>
| |
| - | <td class="style2">
| |
| - | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#10-10-2010">
| |
| - | 10-10-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">41</td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#11-10-2010">11-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#12-10-2010">12-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#13-10-2010">13-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#14-10-2010">14-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#15-10-2010">15-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#16-10-2010">16-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#17-10-2010">17-10-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">42</td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#18-10-2010">18-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#19-10-2010">19-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#20-10-2010">20-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#21-10-2010">21-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#22-10-2010">22-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#23-10-2010">23-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#24-10-2010">24-10-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">43</td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#25-10-2010">25-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#26-10-2010">26-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#27-10-2010">27-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#28-10-2010">28-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#29-10-2010">29-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#30-10-2010">30-10-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#31-10-2010">31-10-2010</a></td>
| |
| - | </tr>
| |
| - | <tr>
| |
| - | <td class="style3">44</td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#01-11-2010">01-11-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#02-11-2010">02-11-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#03-11-2010">03-11-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#04-11-2010">04-11-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#05-11-2010">05-11-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#06-11-2010">06-11-2010</a></td>
| |
| - | <td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#07-11-2010">07-11-2010</a></td>
| |
| - | </tr>
| |
| - | </table>
| |
| - | </html>
| |
| | | | |
| - | =12-07-2010=
| |
| - | * OD measurement of Asaia’s culture
| |
| - | * Chemical competence for E.Coli DB3.1 (step : Day 3)
| |
| - | * PCR (Asaia ORI + primers)=12-07-2010=
| |
| - | * OD measurement of Asaia’s culture
| |
| - | * Chemical competence for E.Coli DB3.1 (step : Day 3)
| |
| - | * PCR (Asaia ORI + primers)
| |
| - | * Made GLY agar plates without antibiotics -> failed (medium was too old)
| |
| - | * Asaia O/N culture without antibiotics in order to recover some WT
| |
| - | * Text for the sponsor
| |
| - |
| |
| - |
| |
| - | =13-07-2010=
| |
| - | * Chemical competence for E.Coli DB3.1 (step : Day 4) -> stored at -80°C
| |
| - | * Preparation of GLY Medium / LB Medium / SOC Medium / TAE 1X / TAE 0.5X
| |
| - | * Run a gel for the PCR -> failed (mix of two different kits)
| |
| - | * Preparation of plates (GLY Agar / LB+Kan Agar / LB+Amp Agar)
| |
| - | * Learned to autoclave
| |
| - | * Restarted PCR (Asaia ORI + primers)
| |
| - | * Plated Asaia (O/N culture)
| |
| - | * Choice of Antibiotics Biobricks resistance (Kan / Amp / Tet / Chl) from the iGem Kit and preparation of them (streak out)
| |
| - | * Transformation of E.coli DB3.1 with pUC19 to check its competence
| |
| - | * Transformation of E.coli DH5 with p1003 (Kan) / p1002 (Amp) / p1004 (Chl) / p1005 (Tet)
| |
| - | * E.coli DB3.1 and DH5 were plated on LB+Amp Agar plates (Kan was plated on LB+Kan Agar plate)
| |
| - | * Ordered material
| |
| - | * Wiki brainstorming
| |
| - | * Protocols printing and organization
| |
| | | | |
| | | | |
| - | =14-07-2010=
| |
| - | * Run a gel for the PCR of the previous day -> worked
| |
| - | * Purification of PCR’s product -> ok
| |
| - | * Preparation of the BBa_151020 (streak out)
| |
| - | * Competence Calculation of E.Coli DB3.1 (+pUC10, Amp) -> ok
| |
| - | * Look at Asaia with microscope -> we have cells (pictures)
| |
| - | * Growth Curve for Asaia (OD measurement each hour) -> both manually (ok) + with the plate reader
| |
| - | * Plated Asaia to recover some WT (streak out)
| |
| - | * Preparation of Asaia Culture (for Lemaître experiment) with Kan
| |
| - | * Protocol LateX template
| |
| - | * O/N culture of the E.coli DH5 transformed with the BB Antibiotics resistance (4x) -> miniprep
| |
| - | * Learning HTML for the wiki
| |
| - | * Text for the project presentation due to iGem
| |
| - | * Writing Protocols of basic procedures for the wiki page
| |
| - | * Culture of Asaia + Kan
| |
| | | | |
| | + | <div style="position: absolute; left: 5px; top: 210px; height: 200px; width: 170px;"> |
| | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week5"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/1/19/EPFL_Week05.png"/></a></div> |
| | | | |
| - | =15-07-2010= | + | <div style="position: absolute; left: 230px; top: 200px; height: 200px; width: 170px;"> |
| - | * MaxiPrep of Lemaître’s culture
| + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week6"> |
| - | * Purification of the BB resistances from the E.Coli cultures
| + | <img alt="" src="https://static.igem.org/mediawiki/2010/6/6b/EPFL_Week06.png"/></a></div> |
| - | * Protocol for cloning the Asaia Vector
| + | |
| - | * Enzyme digestion of the BB resistances and Asaia_ORI
| + | |
| - | * Cloning (Asaia_ORI + Vector/ Asaia_ORI + BB + Vector)
| + | |
| - | * Wiki text
| + | |
| - | * Preparation of LB agar + Cm
| + | |
| - | * Electrophoresis of resistance fragments (Works!!!) and extraction
| + | |
| - | * Purification of extracted DNA from agarose gel
| + | |
| | | | |
| | + | <div style="position: absolute; left: 440px; top: 215px; height: 200px; width: 170px;"> |
| | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week7"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/4/48/EPFL_Week07.png"/></a></div> |
| | | | |
| - | =16-07-2010= | + | <div style="position: absolute; left: 640px; top: 210px; height: 200px; width: 170px;"> |
| - | * ligation Asaia origin BB + vector
| + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week8"> |
| - | * ligation Asaia origin BB + Kan + vector
| + | <img alt="" src="https://static.igem.org/mediawiki/2010/2/24/EPFL_Week08.png"/></a></div> |
| - | * ligation Asaia origin BB + Amp + vector
| + | |
| - | * ligation Asaia origin BB + Cm + vector
| + | |
| - | * ligation Asaia origin BB + Tet + vector
| + | |
| - | * Extraction of the OD curve data (values)
| + | |
| - | * Replating Asaia on plates with and without kanamicin in order to recover the WT Asaia (only WT do not grow on kanamicin)
| + | |
| - | * Feeding and infection of Drosophila (WT and immunodeficient redish mutants) with bacteria Ecc15 and Asaia in Prof. Lemaitre Lab
| + | |
| - | * Fluorescence microscopy -> presence of fluorescence in the body/gut as expected
| + | |
| - | * Transformation of E-Coli with the ligation products and plating the transformed bacteria
| + | |
| | | | |
| | | | |
| - | =19-07-2010=
| |
| - | * Results of the transformation checked: successful!!
| |
| - | * Amplification of colonies (goal to do a miniprep of the plasmids --ori-- and --ori-resistance--)
| |
| - | * Results of plating of asaia culture (where we hope to recover the WT) checked: Asaia cultures grew on Kan plates also! Checked colonies for fluorescance under the microscope: no fluorescance --> Very unexpected! Don't know reason yet.
| |
| - | * Re-grow the colonies. Multiple cycles: hope that we get WT.
| |
| - | * Preparation of Asaia cultures for Lemaitre experiments/WT/Competence...
| |
| - | * Preparation of SOC medium and GLY medium
| |
| - | * Autoclaving of media and flasks
| |
| | | | |
| | | | |
| - | =20-07-2010=
| |
| - | * Plating Asaia to get WT (once again)
| |
| - | * Making HEPES Buffer for the Competence of Asaia
| |
| - | * Diluted the culture to get a 25ml culture(1:20) according to Asaia competence protocol
| |
| - | * OD curve plotted, Doubling time calculated
| |
| - | * No single colonies from the re-growing trial --> replating properly on separate plates
| |
| - | * Preparation of LB medium mixed with Kan/Amp/Cm and GLY medium mixed with <kan
| |
| - | * Miniprep of Origin + Resistance
| |
| - | * Miniprep of Origin alone.
| |
| - | * Miniprep of base vector BBa_151020
| |
| - | * Concentration measurments of miniprep products
| |
| - | * Glycerol stock of BB plasmid and Base Vector made
| |
| | | | |
| | | | |
| - | =21-07-2010= | + | <div style="position: absolute; left: 15px; top: 370px; height: 200px; width: 170px;"> |
| - | * Measure OD of overnight culture of Asaia for competence --> value was too high
| + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week9"> |
| - | * Diluted culture to 0.3 OD, left in 30 degree incubator for 2 hours, OD of 0.6 measured --> start with competence
| + | <img alt="" src="https://static.igem.org/mediawiki/2010/3/34/EPFL_Week09.png"/></a></div> |
| - | * Asaia competence
| + | |
| - | * Digestion of BB's produced and of the Base vector
| + | |
| - | * Agar plates with Kan/Tet/Cm/Amp made
| + | |
| - | * Prepared Gel with the products of the digestion to check if the fragments are as expected
| + | |
| - | * Prepare Tet stock (from powder)
| + | |
| | | | |
| | + | <div style="position: absolute; left: 220px; top: 380px; height: 200px; width: 170px;"> |
| | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week10"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/c/ca/EPFL_Week10.png"/></a></div> |
| | | | |
| - | =22-07-2010= | + | <div style="position: absolute; left: 445px; top: 380px; height: 200px; width: 170px;"> |
| - | * Gel with products of digestion run --> only one of the base vector samples and a fragment with the origin and a Kan resistance worked. The others probably did not wok since the concentration of DNA was too low.
| + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week11"> |
| - | * Colonies picked and suspension cultures started. For making a miniprep tomorrow morning.
| + | <img alt="" src="https://static.igem.org/mediawiki/2010/8/86/EPFL_Week11.png"/></a></div> |
| - | * Gel cut, Gel purification made
| + | <!-- |
| - | * New idea: PCR run on the product of the miniprep.
| + | <div style="position: absolute; left: 640px; top: 375px; height: 200px; width: 170px;"> |
| - | * Ligation of Base Vector with Origin+Kan
| + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week12"> |
| - | * Asaia preculture for OD growth (different pH) measurements
| + | <img alt="" src="https://static.igem.org/mediawiki/2010/a/a4/EPFL_Week12.png"/></a></div> |
| - | * Gly medium with pH 2,3,4,5,6,7.
| + | |
| - | * Research
| + | |
| - | * Replating of Asaia (Replating III) to get the WT (one day!!)
| + | |
| | | | |
| | | | |
| - | =23-07-2010=
| |
| - | * OD measurement growth curve in function of pH
| |
| - | * new DNA miniprep to extract Ori+AB_Res (the last one failed...)
| |
| - | * Lyse-n-Go protocol to do the ligase (and to check the insertion)
| |
| - | * transformation of the only digestion from yesterday that didn't failed (Kan)
| |
| - | * meeting with Onya for mosquito experiments
| |
| - | * preparation of 4L of Gly for LeMaitre experiments
| |
| | | | |
| | | | |
| - | =26-07-2010=
| |
| - | * Lemaître experiment: 400 ml cultures of GFP Asaia, Ecc15 and P.Entomophila
| |
| - | * Lemaître experiment: separating male and female drosophila
| |
| - | * Transformation of the Base Vector with -Kan-ori- insert into E.Coli DB3.1
| |
| - | * PCR of LovTab as a positive control to check if the PCR works (with own and Henrike's dNTP's) --> Run gel
| |
| - | * Test-digestion of some of the Mini-preps (Ori alone, Ori-Amp) --> Run gel
| |
| - | * Recovered the wildtype Asaia worked!!
| |
| - | * WT Asaia strains arrived. Culture started
| |
| | | | |
| | + | <div style="position: absolute; left: 10px; top: 540px; height: 200px; width: 170px;"> |
| | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week13"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/8/83/EPFL_Week13.png"/></a></div> |
| | | | |
| - | =27-07-2010= | + | <div style="position: absolute; left: 220px; top: 545px; height: 200px; width: 170px;"> |
| - | * Ligation C3/A3 + Ori + resistance (Kan, Amp, Tet, Cm)
| + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week14"> |
| - | * transformation of the ligation products into E.Coli DH5a
| + | <img alt="" src="https://static.igem.org/mediawiki/2010/e/e5/EPFL_Week14.png"/></a></div> |
| - | * Plating of transformed cells with selection of the vector and the insert
| + | |
| - | * Colony PCR of E.Coli DB3.1 containing BaseVector + Ori + Kan --> running gel (twice, the first time failed)
| + | |
| - | * Preculture of (maybe!!) WT Asaia for competence protocol
| + | |
| - | * Sorting of male/female flies
| + | |
| - | * Culture of 400 ml of different species of bacteria.
| + | |
| | | | |
| | + | <div style="position: absolute; left: 450px; top: 540px; height: 200px; width: 170px;"> |
| | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week15"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/3/3f/EPFL_Week15.png"/></a></div> |
| | | | |
| - | =28-07-2010= | + | <div style="position: absolute; left: 650px; top: 540px; height: 200px; width: 170px;"> |
| - | * Analysis of plating of transformed cells
| + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week16"> |
| - | * Gel for check the Colony PCR
| + | <img alt="" src="https://static.igem.org/mediawiki/2010/4/41/EPFL_Week16.png"/></a></div> |
| - | * 250 ml Gly medium
| + | |
| - | * OD measurement of preculture for competent Asaia --> too high. Start a new one!!!
| + | |
| - | * Competent Asaia and stocking @-80°C
| + | |
| - | * Plating of transformed E.Coli for double check
| + | |
| - | * preparation of the pellets for infection with 3 different species of bacteria + OD measurements
| + | |
| - | * preparation of tubes with 15 female flies
| + | |
| - | * begin of the survival experiment: infection of flies --> check of the flies after 2h
| + | |
| | | | |
| | | | |
| - | =29-07-2010=
| |
| - | * Results from plating of transformed E.Coli for double check and miniprep from some of them
| |
| - | * Digestion with different restriction enzymes to check if we get the right length
| |
| - | * Counting of dead flies one day after the infection
| |
| - | * Observation of fluorescence of infected flies under the microscope
| |
| | | | |
| | + | <div style="position: absolute; left: 370px; top: 680px; height: 200px; width: 170px;"> |
| | + | <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week1"> |
| | + | <img alt="" src="https://static.igem.org/mediawiki/2010/9/90/EPFL_Week17.png"/></a></div> |
| | + | --> |
| | | | |
| - | =30-07-2010=
| + | </html> |
| - | * Running a gel of the digested products of miniprep (29.07) --> bad results
| + | |
| - | * Miniprep for the last 17 samples of replating
| + | |
| - | * Digestion of 5 samples with 3 restriction enzymes to check if we get the fragments we expect
| + | |
| - | * Running a gel for the digestion products
| + | |
| - | * Counting of dead flies two days after the infection
| + | |
| - | * Observation of fluorescence of infected flies under the microscope
| + | |
| | | | |
| | | | |
| - | = 02-08-2010 =
| |
| - | {| cellspacing="15"
| |
| - | | style="width: 33.33%" valign="top" |
| |
| - | == Other ==
| |
| - | * Made some plates with GLY medium and Agar.
| |
| | | | |
| - | | style="width: 33.33%" valign="top" |
| |
| | | | |
| - | == Asaia ==
| |
| - | Today we transform competent Asaia with a GFP + kanamycin (resistance) following this [https://static.igem.org/mediawiki/2010/a/ac/EPFL_prot_Electroporation_asaia.pdf protocols]. We plate it and we'll waiting 2 days to see if it works.
| |
| - | we do digestion to create our first biobrick. We will make the ligation and the transformation in E-Coli tomorrow.
| |
| | | | |
| - | | style="width: 33.33%" valign="top" |
| |
| - | == Drosophile ==
| |
| - | we count the drosophile and take photos with the fluorecense microscope. In some tubes the filter has detached so the expermiment for those tube are finished.
| |
| - | |}
| |
| | | | |
| | | | |
| | | | |
| - | = 02-08-2010 =
| |
| - | {| cellspacing="15"
| |
| - | | style="width: 50%" valign="top" |
| |
| - | == Asaia ==
| |
| - | Ligation/Transformation C3+Promotor(weak/strong)+RBS
| |
| - | Ligation/Transformation C3+Promotor(w/s)+RBS+Immuno
| |
| - | | style="width: 50%" valign="top" |
| |
| | | | |
| - | == BioBricks ==
| |
| - | Digestion/Ligation of ORI + Resistance (Amp/Kan) into Base Vectors
| |
| - | |}
| |
| | | | |
| - | = 03-08-2010 =
| |
| - | {| cellspacing="15"
| |
| - | | style="width: 25%" valign="top" |
| |
| - | == Immunotoxin ==
| |
| - | We have designed primers that overlap, generating a promoter and ribosome binding site. We want to put this in a vector which would be our basis to add other genes, for example our immunotoxin.
| |
| - | We designed a strong promoter+RBS and a weak one, just in case the strong one generates too much immunotoxin and kills the bacteria...
| |
| | | | |
| | | | |
| - | | style="width: 25%" valign="top" |
| |
| | | | |
| - | == Wiki ==
| |
| - | We did a lot of work on the wiki but we haven't downloaded it yet.
| |
| | | | |
| | | | |
| - | | style="width: 25%" valign="top" |
| |
| | | | |
| - | ==Asaia==
| |
| - | We keep counting the drosophila and taking pictures with the GFP filter.
| |
| | | | |
| | | | |
| - | | style="width: 25%" valign="top" |
| |
| | | | |
| - | ==Biobrick==
| |
| | | | |
| - | *Digestion of the plasmid containing the origin + one resitance (either Amp, Kan or Ch) and digestion of the vector i51020
| |
| - | *Ligation ofthe the different parts (one insert in one back bone vector) => origin+resistance+backbone_vector_for_biobrick
| |
| - | *Plating over night => we obtain something on the plate for the ori + amp nothing on the other
| |
| - | |}
| |
| | | | |
| - | = 04-08-2010 =
| |
| - | {| cellspacing="15"
| |
| | | | |
| | | | |
| - | | style="width: 25%" valign="top" |
| |
| - | ==Immunotoxin==
| |
| - | Henrike verified the primer sequences for us and ordered them --> they should arrive on Friday or Monday.
| |
| | | | |
| - | | style="width: 25%" valign="top" |
| |
| - | == Asaia ==
| |
| - | * The asaia transformation we let in the incubator since 02-08 are now showing colonies! As expected and verified under the fluorescent microscope, they express GFP.
| |
| | | | |
| - | * Transformed Asaia with the pB129421248??? plasmid from E. coli to see whether it works in Asaia.
| |
| | | | |
| - | | style="width: 25%" valign="top" |
| |
| | | | |
| - | == Other ==
| |
| - | * Continued counting the dead Drosophilae
| |
| - | * Made 2l of liquid gly medium
| |
| - | * Put 2 5ml cultures of recovered wt-Asaia in the incubator to make competent cells
| |
| | | | |
| | | | |
| - | | style="width: 25%" valign="top" |
| |
| | | | |
| - | == Biobrick ==
| |
| - | * Make 5 ml miniprep to verify the ori + ch + i5010
| |
| - | * Make negativ control for the ori + ch + i5010 on Lb+Amp plate (as the ori + amp were in the A3 backbonevector)
| |
| - | |}
| |
| | | | |
| - | = 05-08-2010 =
| |
| - | {| cellspacing="15"
| |
| | | | |
| - | | style="width: 33.33%" valign="top" |
| |
| - | == Other ==
| |
| - | * Continued counting the dead Drosophilae and taking pictures.
| |
| - | * Measured the OD of the two 5ml wt-Asaia cultures (4.1 and 4.7),diluted the cultures to obtain a solution with OD 0.6 tomorrow morning and finally make competent cells
| |
| | | | |
| - | | style="width: 33.33%" valign="top" |
| |
| | | | |
| - | == Biobrick ==
| |
| - | *The previous ligation didn't work at all, so we made a new ligation with ori + res + i51020
| |
| - | *We replated the collonies that had well grown on the plate the 28.7 to keep them in glycerol
| |
| | | | |
| - | | style="width: 33.33%" valign="top" |
| |
| | | | |
| - | == Asaia ==
| |
| - | * We made a culture to make them competent toworrow (06.08)
| |
| - | |}
| |
| | | | |
| - | = 06-08-2010 =
| |
| - | {| cellspacing="15"
| |
| | | | |
| - | | style="width: 50%" valign="top" |
| |
| | | | |
| - | == Biobrick ==
| |
| - | * We obtained colonies on the plate from yesterday (Asaia origin + Resistance). We made a miniprep from that and stored some on Glycerol in the -80°C freezer. With the same miniprep we run a gel. Result :
| |
| | | | |
| - | | style="width: 50%" valign="top" |
| |
| | | | |
| - | == Asaia ==
| |
| - | * Measured the OD of our Asaia cultures. They were 1.24 and 1.95. We diluted them again and then started the procedure to make competent cells. Finally we got 22 aliquots of 150ul.
| |
| - | * Had fun with the liquid nitrogen left over...
| |
| - | * Plated one aliquot on Gly without AB to make sure the now hopefully competent cells survived the procedures.
| |
| - | * We made some stock of "asaia origine + Resistance (Kan, Cm, Amp) + BackBones Vector" in the -80°C fridge.
| |
| - | * We've transformed Asaia with plasmid C3 + E-coli Origin + Resistance and the colonie didn't grow so we can say it out and loud :
| |
| | | | |
| - | :: '''''E-coli Origin doesn't work in Asaia '''''
| |
| | | | |
| - | |}
| |
| | | | |
| - | = 06-08-2010 =
| |
| - | {| cellspacing="15"
| |
| | | | |
| - | | style="width: 50%" valign="top" |
| |
| | | | |
| - | == Biobrick ==
| |
| - | * We obtained colonies on the plate from yesterday (Asaia origin + Resistance). We made a miniprep from that and stored some on Glycerol in the -80°C freezer. With the same miniprep we run a gel. Result :
| |
| | | | |
| - | | style="width: 50%" valign="top" |
| |
| | | | |
| - | == Asaia ==
| |
| - | * Measured the OD of our Asaia cultures. They were 1.24 and 1.95. We diluted them again and then started the procedure to make competent cells. Finally we got 22 aliquots of 150ul.
| |
| - | * Had fun with the liquid nitrogen left over...
| |
| - | * Plated one aliquot on Gly without AB to make sure the now hopefully competent cells survived the procedures.
| |
| - | * We made some stock of "asaia origine + Resistance (Kan, Cm, Amp) + BackBones Vector" in the -80°C fridge.
| |
| - | * We've transformed Asaia with plasmid C3 + E-coli Origin + Resistance and the colonie didn't grow so we can say it out and loud :
| |
| | | | |
| - | :: '''''E-coli Origin doesn't work in Asaia '''''
| |
| | | | |
| - | |}
| |
| | | | |
| - | = 09-08-2010 =
| |
| - | {| cellspacing="15"
| |
| | | | |
| - | | style="width: 50%" valign="top" |
| |
| | | | |
| - | == Biobrick ==
| |
| | | | |
| - | | style="width: 50%" valign="top" |
| |
| | | | |
| - | == Asaia ==
| |
| - | * We made pre-culture and plates (on both Kan and Gly alone) of the recovered WT Asaia
| |
| - | * Made PCR of the promotor + RBS and ran gel -> it worked
| |
| | | | |
| - | |}
| |
| | | | |
| - | = 10-08-2010 =
| |
| - | {| cellspacing="15"
| |
| - | | style="width: 50%" valign="top" |
| |
| - | == Immunotoxine ==
| |
| - | We'll made some GLY stock of E-coli containing the immunotoxine plasmid (shiva). So today we transform E-coli with the plasmid and we plated on Amp overnight.
| |
| | | | |
| - | | style="width: 50%" valign="top" |
| |
| - | == Biobrick ==
| |
| - | We try to begin to construc two new biobrick today but we made mistake. We'll start again tomorrow.
| |
| - |
| |
| - | |}
| |
| | | | |
| | + | = How to use the Notebook = |
| | | | |
| | + | This is a NOTEbook and not a LABbook. Here you can describe what you have done during your exciting day. If you want to have more details you can look in the IRL Labbook. Use ctrl+F to find something ;) |
| | | | |
| | + | == template of the day == |
| | + | <html> <table cellspacing="15" > <tr> <td> </html> |
| | + | For each new day in the lab you must create a new date in the notebook. To do so, copy-paste the following template: |
| | | | |
| - | = 11-08-2010 = | + | <pre> |
| | + | = 02-08-2010 = |
| | {| cellspacing="15" | | {| cellspacing="15" |
| - | | style="width: 25%" valign="top" | | + | | style="width: 33.33%" valign="top" | |
| - | == Immunotoxine == | + | == Other == |
| - | We've pick up some colony of transformed cells from yesterday and make liquid culutre of them. Tomorrow we'll make some GLY stock and a gel to control. With this stock we can begin to work safely with the immunotoxine.
| + | It rain a lot today |
| | | | |
| - | | style="width: 25%" valign="top" | | + | | style="width: 33.33%" valign="top" | |
| | == Asaia == | | == Asaia == |
| - | To test our asaia origin sequence, we take a BV with (1) asaia origin (2) Amp resistance (3) death casette and we will throw away the death casette and just transform asaia with this simpel plasmid. Today we just make a liquid culture from a GLY stock with E-coli containing this plasmid.
| + | Today I speak with an asaia and she answer to me ^^ |
| | | | |
| - | | style="width: 25%" valign="top" | | + | | style="width: 33.33%" valign="top" | |
| - | == Biobricks ==
| + | |
| - | We begin to construc a new Biobrick containing (1) Base Vector (2) Strong or Weak promotor (3) RBS. Today we made digestion, ligation and E-coli transformation. Plated overnight on Amp.
| + | |
| - | | + | |
| - | | style="width: 25%" valign="top" |
| + | |
| | == Drosophile == | | == Drosophile == |
| - | '''Persistence of bacteria in drosophilia's gut.''' We infected (fed) flies with Asaia. We also infected flies with persistant or pathogenic bacteria (Pseudomonas in control experiment). Then we crushed, diluted and plated infected flies after 3h hours from infection. We will plate flies after 24 and 48 hours as well and see how many colonies appear on the plates. We hope to get an idea of the persistency of Asaia in the gut of Drosophila.
| |
| - | |}
| |
| - |
| |
| - |
| |
| - |
| |
| - | = 12-08-2010 =
| |
| - | {| cellspacing="15"
| |
| - |
| |
| - | | style="width: 50%" valign="top" |
| |
| - | == Drosophila ==
| |
| - | * Same thing as yesterday afternoon, Making plates to get the CFU-count
| |
| - | * Plates of yesterday with Pseudomonas look promising, the cells grew and the dilution was there.
| |
| - |
| |
| - | | style="width: 50%" valign="top" |
| |
| - | == Biobrick ==
| |
| - | Digestion of Immunotoxine
| |
| - | Other jobs failed and we discovered we have to restart many digestion/ligation
| |
| - | Digestion C3+Promotor(weak/strong)+RBS
| |
| - | Digestion C3+Promotor(w/s)+RBS+Immuno
| |
| - |
| |
| - | |}
| |
| - |
| |
| - | = 13-08-2010 =
| |
| - | {| cellspacing="15"
| |
| - |
| |
| - | | style="width: 33%" valign="top" |
| |
| - | == Drosophilae ==
| |
| - | * Continued yesterday's experiment with plating, this time the 48h persistence.
| |
| - | * Unfortunately the plates from yesterday don't look good. We might have killed all the bacteria by washing the flies in ethanol for too long.
| |
| - |
| |
| - | | style="width: 33%" valign="top" |
| |
| - | == Asaia ==
| |
| - | * Prepared a wild type recovery pre-culture to obtain competent cells. We're repeating the procedure from two weeks ago, because what we obtained then didn't seem to be the wild type (it grew on plates with Kanamycin).
| |
| - |
| |
| - | | style="width: 33%" valign="top" |
| |
| - | == Biobrick ==
| |
| - | Digestion of Immunotoxine
| |
| - | Other jobs failed and we discovered we have to restart many digestion/ligation
| |
| - | Digestion C3+Promotor(weak/strong)+RBS
| |
| - | Digestion C3+Promotor(w/s)+RBS+Immuno
| |
| - |
| |
| - | |}
| |
| - |
| |
| - |
| |
| - |
| |
| - |
| |
| - | = 14-08-2010 =
| |
| - | {| cellspacing="15"
| |
| - | | style="width: 50%" valign="top" |
| |
| - | == BioBricks ==
| |
| - | Today we came to check our culture of E-coli transformed with the plasmid C3 + Promotor + RBS (+ Immunotoxine) but no plate have grown!!
| |
| - | | style="width: 50%" valign="top" |
| |
| - | == Asaia ==
| |
| - | We also transformed E-coli 3.1 with the Base Vector (BBa_I51020) + Asaia Origin + Resistance.
| |
| | | | |
| | |} | | |} |
| | + | </pre> |
| | | | |
| - | = 15-08-2010 =
| + | <html> </td><td> </html> |
| - | {| cellspacing="15"
| + | |
| - | | style="width: 100%" valign="top" |
| + | |
| - | == Asaia ==
| + | |
| - | Today we just come and check our transformed cells of yesterday. We have colonies on two plates. Work continue tomorrow.
| + | |
| - |
| + | |
| - | |}
| + | |
| | | | |
| - | = 16-08-2010 =
| + | <u>Here are some useful explanations : </u> |
| - | {| cellspacing="15"
| + | |
| - | | style="width: 100%" valign="top" |
| + | |
| - | == Asaia ==
| + | |
| - | * transferred pre-culture for competent wild-type into flasks, diluted 1:50
| + | |
| - |
| + | |
| - | == BioBrick ==
| + | |
| | | | |
| - | * Digestion / Ligation of C3 vector with either a strong or a weak promoter, with and without Immunotoxin
| + | 1. The date format is important! |
| - | |}
| + | |
| | | | |
| - | = 17-08-2010 =
| + | 2. Just after the date, put the following two lines : |
| - | {| cellspacing="15" | + | <pre> |
| - | | style="width: 100%" valign="top" | | + | {| cellspacing="15" |
| - | == Asaia == | + | | style="width: 33.33%" valign="top" | |
| - | * Made about 30 aliquots (150ul) of competent wt-cells and stored them at -80°C. Using liquid nitrogen is fun!
| + | </pre> |
| | + | 3. Beetween each subtitle, put this line : |
| | + | <pre> |
| | + | | style="width: 33.33%" valign="top" | |
| | + | </pre> |
| | + | 4. To end the day just put : |
| | + | <pre> |
| | + | |} |
| | + | </pre> |
| | + | 5. Of course the text "width:33.33%" must be adapted on how many subtitle you have (for instance 4 subtitles = 25%) |
| | | | |
| - | == BioBrick ==
| + | <html></td></tr></table></html> |
| - | * Transformation of E.Coli DH5alpha with the previous day's ligations
| + | |
| - | * Running a gel to check if the Base Vector with ORI (asaia) and a resistance were fine, no concluant results
| + | |
| - | * Liquid cultures of the colonies (E.Coli transformed with Base Vector + ORI (Asaia) + Res. )
| + | |
| - |
| + | |
| - | |}
| + | |
| | | | |
| - | = 18-08-2010 =
| |
| - | {| cellspacing="15"
| |
| - | | style="width: 100%" valign="top" |
| |
| - | == Biobricks ==
| |
| - | * Plasmid miniprep from the previous day's liquid culture
| |
| - | * Digestion and gel to check if the Base Vector + ORI + Res were rights -> didn't work
| |
| - | * Successful transformation -> Liquid cultures of the E.Coli DH5alpha colonies (C3 vector)
| |
| - | * Colony PCR (didn't work)
| |
| - | * Restarting the digestion/ligation of the Base Vector with the ORI (Asaia) and Amp or Kan resistance
| |
| - | * PCR of the p25 and p28 proteins
| |
| - | * Gel to check the PCR (failed)
| |
| | | | |
| - | == Drosophilae ==
| |
| | | | |
| - | * We're redoing the same experiment as last week: starving the flies for 2-3hours, then transferring them to tubes with food with bacteria. This time we were more careful when drowning the flies in Ethanol (never more than 5 seconds, to make sure the bacteria in the gut survive). We also added a fourth strain, Ecc-15, to the experiment.
| |
| - | * We also did the 3h survivance test by plating the crushed drosophilae on gly and LB medium.
| |
| - |
| |
| - | |}
| |
| - | = 25-08-2010 =
| |
| - | {| cellspacing="15"
| |
| - | | style="width: 33.33%" valign="top" |
| |
| - | == Biobricks ==
| |
| - | The plasmids we sent to sequencing came back positive. We have now 4 biobricks.
| |
| - | # C3 + Asaia_origin + Kan
| |
| - | # C3 + Asaia_origin + Amp
| |
| - | # C3 + weak promoter + RBS
| |
| - | # C3 + strong promoter + RBS
| |
| | | | |
| - | We also ran a gel with C3 + asaia origin. It looks good.
| + | {{EPFL_2010_bottom_wrap}} |
| | | | |
| - | | style="width: 33.33%" valign="top" |
| + | </div> |
| - | == Asaia ==
| + | |
| - | | + | |
| - | | style="width: 33.33%" valign="top" |
| + | |
| - | == Drosophilae ==
| + | |
| - | We restarted the experiment with the drosophila. This time, we crushed 15 flies and we washed them in ethanol for only 2 seconds (maximum).
| + | |
| - | We also did the plating of the flies 3h after infection
| + | |
| - | |}
| + | |
| - | | + | |
| - | = 26-08-2010 =
| + | |
| - | {| cellspacing="15"
| + | |
| - | | style="width: 33.33%" valign="top" |
| + | |
| - | == Biobricks ==
| + | |
| - | We did a digestion and ligation of our 2 vectors C3 + S and C3 + W with the immunotoxin, P25 and P26. In the end, we should have 6 new biobricks.
| + | |
| - | | + | |
| - | | style="width: 33.33%" valign="top" |
| + | |
| - | == Asaia ==
| + | |
| - | We are still not sure if Asaia "WT" is resistant to kanamycin or not. Maybe we will have to use another resistance in our biobricks, TetR for instance.
| + | |
| - | | + | |
| - | | style="width: 33.33%" valign="top" |
| + | |
| - | == Drosophilae ==
| + | |
| - | We are doing the crushing and plating of the drosophilae that were infected 24h ago.
| + | |
| - |
| + | |
| - | |}
| + | |
"
