Team:EPF Lausanne/Notebook

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<img src="https://static.igem.org/mediawiki/2010/7/79/EPFL_title_picture_Lab_book.jpg" style="position: relative; top: -87px; left:-5px;"/>
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<div style="position: absolute; left: 0px; top: 60px; height: 140px; width: 975px;">
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<img alt="" src="https://static.igem.org/mediawiki/2010/0/0f/EPFL_Title_2_Notebook.png"/></div>
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<div style="position: absolute; left: 435px; top: 11px; height: 85px; width: 125px;">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook">
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<img alt="" height="80" src="https://static.igem.org/mediawiki/2010/b/b9/EPFL_Menu_2_Notebook.png" width="125" class="auto-style1" /></a></div>  
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{{EPFL_sub_menu_notebook}}
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<div CLASS="EPFL_content">
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<head>
 
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<style type="text/css">
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<div style="position: absolute; left: 10px; top: 50px; height: 200px; width: 170px;">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week1">
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<img alt="" src="https://static.igem.org/mediawiki/2010/b/bd/EPFL_Week01.png"/></a></div>  
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.toc {
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<div style="position: absolute; left: 220px; top: 60px; height: 200px; width: 170px;">
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    display:none;
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week2">
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}
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<img alt="" src="https://static.igem.org/mediawiki/2010/3/3d/EPFL_Week02.png"/></a></div>
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.EPFL_content {
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<div style="position: absolute; left: 430px; top: 45px; height: 200px; width: 170px;">
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          margin-left:0px;
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week3">
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}
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<img alt="" src="https://static.igem.org/mediawiki/2010/c/c9/EPFL_Week03.png"/></a></div>
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<div style="position: absolute; left: 640px; top: 55px; height: 200px; width: 170px;">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week4">
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<img alt="" src="https://static.igem.org/mediawiki/2010/f/fc/EPFL_Week04.png"/></a></div>
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.style1 {
 
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</style>
 
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</head>
 
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</html>
 
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<div CLASS="EPFL_content">
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<div style="position: absolute; left: 5px; top: 210px; height: 200px; width: 170px;">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week5">
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<img alt="" src="https://static.igem.org/mediawiki/2010/1/19/EPFL_Week05.png"/></a></div>  
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= How to use the Notebook =
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<div style="position: absolute; left: 230px; top: 200px; height: 200px; width: 170px;">
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This is a NOTEbook and not a LABbook. Here you can describe what you have done during your exiting day. If you want to have more details you can just pic up the date and take a look in the IRL Labbook. Think to ctrl+F to find something ;)
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week6">
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<img alt="" src="https://static.igem.org/mediawiki/2010/6/6b/EPFL_Week06.png"/></a></div>
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== template of a day ==
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<div style="position: absolute; left: 440px; top: 215px; height: 200px; width: 170px;">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week7">
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<img alt="" src="https://static.igem.org/mediawiki/2010/4/48/EPFL_Week07.png"/></a></div>
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For each new day in the lab you must create a new date in the notebook. Please copy-past the following template.
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<div style="position: absolute; left: 640px; top: 210px; height: 200px; width: 170px;">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week8">
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<img alt="" src="https://static.igem.org/mediawiki/2010/2/24/EPFL_Week08.png"/></a></div>
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= 02-08-2010 =
 
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{| cellspacing="15"
 
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| style="width: 33.33%" valign="top" |
 
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== Other ==
 
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It rain a lot today
 
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| style="width: 33.33%" valign="top" |
 
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== Asaia ==
 
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Today I speak with an asaia and she answer to me ^^
 
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| style="width: 33.33%" valign="top" |
 
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== Drosophile ==
 
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|}
 
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Here is some explanation :
 
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# The date format is important!
 
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# Just after the date, put this two lines :
 
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{| cellspacing="15"
 
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| style="width: 33.33%" valign="top" |
 
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# Beetween each subtitle, put this line :
 
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| style="width: 33.33%" valign="top" |
 
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# To end the day just put :
 
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|}
 
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# Of course the text "width:33.33%" must be adapted on how many subtitle you have (for instance 4 subtitles = 25%)
 
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= Calendar =
 
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<html>
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<div style="position: absolute; left: 15px; top: 370px; height: 200px; width: 170px;">
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<table style="width: 100%">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week9">
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<tr class="style4">
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<img alt="" src="https://static.igem.org/mediawiki/2010/3/34/EPFL_Week09.png"/></a></div>  
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<td>Week</td>
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<td>Monday</td>
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<td>Tuesday</td>
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<td>Wednesday</td>
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<td>Thursday</td>
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<td>Friday</td>
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<td>Satursday</td>
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<td>Sunday</td>
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</tr>
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<tr>
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<td class="style3">28</td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#12-07-2010">
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12-07-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#13-07-2010">
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13-07-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#14-07-2010">
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14-07-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#15-07-2010">
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15-07-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#16-07-2010">
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16-07-2010</a></td>
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<td class="style2">
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17-07-2010</td>
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<div style="position: absolute; left: 220px; top: 380px; height: 200px; width: 170px;">
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week10">
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<img alt="" src="https://static.igem.org/mediawiki/2010/c/ca/EPFL_Week10.png"/></a></div>  
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18-07-2010</td>
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<div style="position: absolute; left: 445px; top: 380px; height: 200px; width: 170px;">
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</tr>
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week11">
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<tr>
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<img alt="" src="https://static.igem.org/mediawiki/2010/8/86/EPFL_Week11.png"/></a></div>  
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<td class="style3">29</td>
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<!--
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<td class="style2">
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<div style="position: absolute; left: 640px; top: 375px; height: 200px; width: 170px;">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#19-07-2010">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week12">
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19-07-2010</a></td>
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<img alt="" src="https://static.igem.org/mediawiki/2010/a/a4/EPFL_Week12.png"/></a></div>  
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#20-07-2010">
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20-07-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#21-07-2010">
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21-07-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#22-07-2010">
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22-07-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#23-07-2010">
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23-07-2010</a></td>
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<td class="style2">
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-
24-07-2010</td>
 
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<td class="style2">
 
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25-07-2010</td>
 
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</tr>
 
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<tr>
 
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<td class="style3">30</td>
 
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<td class="style2">
 
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#26-07-2010">
 
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26-07-2010</a></td>
 
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<td class="style2">
 
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#27-07-2010">
 
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27-07-2010</a></td>
 
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<td class="style2">
 
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#28-07-2010">
 
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28-07-2010</a></td>
 
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<td class="style2">
 
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#29-07-2010">
 
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29-07-2010</a></td>
 
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<td class="style2">
 
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#30-07-2010">
 
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30-07-2010</a></td>
 
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<td class="style2">
 
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31-07-2010</td>
 
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<td class="style2">
 
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01-08-2010</td>
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</tr>
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<div style="position: absolute; left: 10px; top: 540px; height: 200px; width: 170px;">
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<tr>
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week13">
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<td class="style3">31</td>
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<img alt="" src="https://static.igem.org/mediawiki/2010/8/83/EPFL_Week13.png"/></a></div>  
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#02-08-2010">
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<div style="position: absolute; left: 220px; top: 545px; height: 200px; width: 170px;">
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02-08-2010</a></td>
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week14">
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<td class="style2">
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<img alt="" src="https://static.igem.org/mediawiki/2010/e/e5/EPFL_Week14.png"/></a></div>  
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#03-08-2010">
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03-08-2010</a></td>
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<div style="position: absolute; left: 450px; top: 540px; height: 200px; width: 170px;">
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week15">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#04-08-2010">
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<img alt="" src="https://static.igem.org/mediawiki/2010/3/3f/EPFL_Week15.png"/></a></div>  
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04-08-2010</a></td>
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<td class="style2">
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<div style="position: absolute; left: 650px; top: 540px; height: 200px; width: 170px;">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#05-08-2010">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week16">
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05-08-2010</a></td>
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<img alt="" src="https://static.igem.org/mediawiki/2010/4/41/EPFL_Week16.png"/></a></div>  
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#06-08-2010">
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06-08-2010</a></td>
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<td class="style2">
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<div style="position: absolute; left: 370px; top: 680px; height: 200px; width: 170px;">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#07-08-2010">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook/week1">
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07-08-2010</a></td>
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<img alt="" src="https://static.igem.org/mediawiki/2010/9/90/EPFL_Week17.png"/></a></div>  
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<td class="style2">
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-->
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#08-08-2010">
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08-08-2010</a></td>
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-
</tr>
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<tr>
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<td class="style3">32</td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#09-08-2010">
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09-08-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#10-08-2010">
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10-08-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#11-08-2010">
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11-08-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#12-08-2010">
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12-08-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#13-08-2010">
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13-08-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#14-08-2010">
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14-08-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#15-08-2010">
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15-08-2010</a></td>
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</tr>
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-
<tr>
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<td class="style3">33</td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#16-08-2010">
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16-08-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#17-08-2010">
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17-08-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#18-08-2010">
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18-08-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#19-08-2010">
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19-08-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#20-08-2010">
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20-08-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#21-08-2010">
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21-08-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#22-08-2010">
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22-08-2010</a></td>
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-
</tr>
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-
<tr>
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<td class="style3">34</td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#23-08-2010">
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23-08-2010</a></td>
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-
<td class="style2">
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-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#24-08-2010">
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24-08-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#25-08-2010">
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25-08-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#26-08-2010">
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26-08-2010</a></td>
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-
<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#27-08-2010">
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27-08-2010</a></td>
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<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#28-08-2010">
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28-08-2010</a></td>
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<td class="style2">
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-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#29-08-2010">
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29-08-2010</a></td>
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-
</tr>
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-
<tr>
+
-
<td class="style3">35</td>
+
-
<td class="style2">
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-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#30-08-2010">
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-
30-08-2010</a></td>
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-
<td class="style2">
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-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#31-08-2010">
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31-08-2010</a></td>
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-
<td class="style2">
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-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#01-09-2010">
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01-09-2010</a></td>
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-
<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#02-09-2010">
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02-09-2010</a></td>
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-
<td class="style2">
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-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#03-09-2010">
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03-09-2010</a></td>
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-
<td class="style2">
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-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#04-09-2010">
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04-09-2010</a></td>
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-
<td class="style2">
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-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#05-09-2010">
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-
05-09-2010</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="style3">36</td>
+
-
<td class="style2">
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-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#06-09-2010">
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-
06-09-2010</a></td>
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-
<td class="style2">
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-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#07-09-2010">
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07-09-2010</a></td>
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-
<td class="style2">
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<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#08-09-2010">
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08-09-2010</a></td>
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-
<td class="style2">
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-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#09-09-2010">
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-
09-09-2010</a></td>
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-
<td class="style2">
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-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#10-09-2010">
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-
10-09-2010</a></td>
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-
<td class="style2">
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-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#11-09-2010">
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11-09-2010</a></td>
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-
<td class="style2">
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-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#12-09-2010">
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12-09-2010</a></td>
+
-
</tr>
+
-
<tr>
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-
<td class="style3">37</td>
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-
<td class="style2">
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-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#13-09-2010">
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-
13-09-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#14-09-2010">
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-
14-09-2010</a></td>
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-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#15-09-2010">
+
-
15-09-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#16-09-2010">
+
-
16-09-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#17-09-2010">
+
-
17-09-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#18-09-2010">
+
-
18-09-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#19-09-2010">
+
-
19-09-2010</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="style3">38</td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#20-09-2010">
+
-
20-09-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#21-09-2010">
+
-
21-09-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#22-09-2010">
+
-
22-09-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#23-09-2010">
+
-
23-09-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#24-09-2010">
+
-
24-09-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#25-09-2010">
+
-
25-09-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#26-09-2010">
+
-
26-09-2010</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="style3">39</td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#27-09-2010">
+
-
27-09-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#28-09-2010">
+
-
28-09-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#29-09-2010">
+
-
29-09-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#30-09-2010">
+
-
30-09-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#01-10-2010">
+
-
01-10-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#02-10-2010">
+
-
02-10-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#03-10-2010">
+
-
03-10-2010</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="style3">40</td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#04-10-2010">
+
-
04-10-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#05-10-2010">
+
-
05-10-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#06-10-2010">
+
-
06-10-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#07-10-2010">
+
-
07-10-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#08-10-2010">
+
-
08-10-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#09-10-2010">
+
-
09-10-2010</a></td>
+
-
<td class="style2">
+
-
<a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#10-10-2010">
+
-
10-10-2010</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="style3">41</td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#11-10-2010">11-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#12-10-2010">12-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#13-10-2010">13-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#14-10-2010">14-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#15-10-2010">15-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#16-10-2010">16-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#17-10-2010">17-10-2010</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="style3">42</td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#18-10-2010">18-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#19-10-2010">19-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#20-10-2010">20-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#21-10-2010">21-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#22-10-2010">22-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#23-10-2010">23-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#24-10-2010">24-10-2010</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="style3">43</td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#25-10-2010">25-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#26-10-2010">26-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#27-10-2010">27-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#28-10-2010">28-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#29-10-2010">29-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#30-10-2010">30-10-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#31-10-2010">31-10-2010</a></td>
+
-
</tr>
+
-
<tr>
+
-
<td class="style3">44</td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#01-11-2010">01-11-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#02-11-2010">02-11-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#03-11-2010">03-11-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#04-11-2010">04-11-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#05-11-2010">05-11-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#06-11-2010">06-11-2010</a></td>
+
-
<td class="style2"> <a href="https://2010.igem.org/Team:EPF_Lausanne/Notebook#07-11-2010">07-11-2010</a></td>
+
-
</tr>
+
-
</table>
+
</html>
</html>
-
=12-07-2010=
 
-
* OD measurement of Asaia’s culture
 
-
* Chemical competence for E.Coli DB3.1 (step : Day 3)
 
-
* PCR (Asaia ORI + primers)=12-07-2010=
 
-
* OD measurement of Asaia’s culture
 
-
* Chemical competence for E.Coli DB3.1 (step : Day 3)
 
-
* PCR (Asaia ORI + primers)
 
-
* Made GLY agar plates without antibiotics -> failed (medium was too old)
 
-
* Asaia O/N culture without antibiotics in order to recover some WT
 
-
* Text for the sponsor
 
-
 
-
 
-
=13-07-2010=
 
-
* Chemical competence for E.Coli DB3.1 (step : Day 4) -> stored at -80°C
 
-
* Preparation of GLY Medium / LB Medium / SOC Medium / TAE 1X / TAE 0.5X
 
-
* Run a gel for the PCR -> failed (mix of two different kits)
 
-
* Preparation of plates (GLY Agar / LB+Kan Agar / LB+Amp Agar)
 
-
* Learned to autoclave
 
-
* Restarted PCR (Asaia ORI + primers)
 
-
* Plated Asaia (O/N culture)
 
-
* Choice of Antibiotics Biobricks resistance (Kan / Amp / Tet / Chl) from the iGem Kit and preparation of them (streak out)
 
-
* Transformation of E.coli DB3.1 with pUC19 to check its competence
 
-
* Transformation of E.coli DH5 with p1003 (Kan) / p1002 (Amp) / p1004 (Chl) / p1005 (Tet)
 
-
* E.coli DB3.1 and DH5 were plated on LB+Amp Agar plates (Kan was plated on LB+Kan Agar plate)
 
-
* Ordered material
 
-
* Wiki brainstorming
 
-
* Protocols printing and organization
 
-
=14-07-2010=
 
-
* Run a gel for the PCR of the previous day -> worked
 
-
* Purification of PCR’s product -> ok
 
-
* Preparation of the BBa_151020 (streak out)
 
-
* Competence Calculation of E.Coli DB3.1 (+pUC10, Amp) -> ok
 
-
* Look at Asaia with microscope -> we have cells (pictures)
 
-
* Growth Curve for Asaia (OD measurement each hour) -> both manually (ok) + with the plate reader
 
-
* Plated Asaia to recover some WT (streak out)
 
-
* Preparation of Asaia Culture (for Lemaître experiment) with Kan
 
-
* Protocol LateX template
 
-
* O/N culture of the E.coli DH5 transformed with the BB Antibiotics resistance (4x) -> miniprep
 
-
* Learning HTML for the wiki
 
-
* Text for the project presentation due to iGem
 
-
* Writing Protocols of basic procedures for the wiki page
 
-
* Culture of Asaia + Kan
 
-
=15-07-2010=
 
-
* MaxiPrep of Lemaître’s culture
 
-
* Purification of the BB resistances from the E.Coli cultures
 
-
* Protocol for cloning the Asaia Vector
 
-
* Enzyme digestion of the BB resistances and Asaia_ORI
 
-
* Cloning (Asaia_ORI + Vector/ Asaia_ORI + BB + Vector)
 
-
* Wiki text
 
-
* Preparation of LB agar + Cm
 
-
* Electrophoresis of resistance fragments (Works!!!) and extraction
 
-
* Purification of extracted DNA from agarose gel
 
-
=16-07-2010=
 
-
* ligation Asaia origin BB + vector
 
-
* ligation Asaia origin BB + Kan + vector
 
-
* ligation Asaia origin BB + Amp + vector
 
-
* ligation Asaia origin BB + Cm + vector
 
-
* ligation Asaia origin BB + Tet + vector
 
-
* Extraction of the OD curve data (values)
 
-
* Replating Asaia on plates with and without kanamicin in order to recover the WT Asaia (only WT do not grow on kanamicin)
 
-
* Feeding and infection of Drosophila (WT and immunodeficient redish mutants) with bacteria Ecc15 and Asaia in Prof. Lemaitre Lab
 
-
* Fluorescence microscopy -> presence of fluorescence in the body/gut as expected
 
-
* Transformation of E-Coli with the ligation products and plating the transformed bacteria
 
-
=19-07-2010=
 
-
* Results of the transformation checked: successful!!
 
-
* Amplification of colonies (goal to do a miniprep of the plasmids --ori-- and --ori-resistance--)
 
-
* Results of plating of asaia culture (where we hope to recover the WT) checked: Asaia cultures grew on Kan plates also! Checked colonies for fluorescance under the microscope: no fluorescance --> Very unexpected! Don't know reason yet.
 
-
* Re-grow the colonies. Multiple cycles: hope that we get WT.
 
-
* Preparation of Asaia cultures for Lemaitre experiments/WT/Competence...
 
-
* Preparation of SOC medium and GLY medium
 
-
* Autoclaving of media and flasks
 
-
=20-07-2010=
 
-
* Plating Asaia to get WT (once again)
 
-
* Making HEPES Buffer for the Competence of Asaia
 
-
* Diluted the culture to get a 25ml culture(1:20) according to Asaia competence protocol
 
-
* OD curve plotted, Doubling time calculated
 
-
* No single colonies from the re-growing trial --> replating properly on separate plates
 
-
* Preparation of LB medium mixed with Kan/Amp/Cm and GLY medium mixed with <kan
 
-
* Miniprep of Origin + Resistance
 
-
* Miniprep of Origin alone.
 
-
* Miniprep of base vector BBa_151020
 
-
* Concentration measurments of miniprep products
 
-
* Glycerol stock of BB plasmid and Base Vector made
 
-
=21-07-2010=
 
-
* Measure OD of overnight culture of Asaia for competence --> value was too high
 
-
* Diluted culture to 0.3 OD, left in 30 degree incubator for 2 hours, OD of 0.6 measured --> start with competence
 
-
* Asaia competence
 
-
* Digestion of BB's produced and of the Base vector
 
-
* Agar plates with Kan/Tet/Cm/Amp made
 
-
* Prepared Gel with the products of the digestion to check if the fragments are as expected
 
-
* Prepare Tet stock (from powder)
 
-
=22-07-2010=
 
-
* Gel with products of digestion run --> only one of the base vector samples and a fragment with the origin and a Kan resistance worked. The others probably did not wok since the concentration of DNA was too low.
 
-
* Colonies picked and suspension cultures started. For making a miniprep tomorrow morning.
 
-
* Gel cut, Gel purification made
 
-
* New idea: PCR run on the product of the miniprep.
 
-
* Ligation of Base Vector with Origin+Kan
 
-
* Asaia preculture for OD growth (different pH) measurements
 
-
* Gly medium with pH 2,3,4,5,6,7.
 
-
* Research
 
-
* Replating of Asaia (Replating III) to get the WT (one day!!)
 
-
=23-07-2010=
 
-
* OD measurement growth curve in function of pH
 
-
* new DNA miniprep to extract Ori+AB_Res (the last one failed...)
 
-
* Lyse-n-Go protocol to do the ligase (and to check the insertion)
 
-
* transformation of the only digestion from yesterday that didn't failed (Kan)
 
-
* meeting with Onya for mosquito experiments
 
-
* preparation of 4L of Gly for LeMaitre experiments
 
-
=26-07-2010=
 
-
* Lemaître experiment: 400 ml cultures of GFP Asaia, Ecc15 and P.Entomophila
 
-
* Lemaître experiment: separating male and female drosophila
 
-
* Transformation of the Base Vector with -Kan-ori- insert into E.Coli DB3.1
 
-
* PCR of LovTab as a positive control to check if the PCR works (with own and Henrike's dNTP's) --> Run gel
 
-
* Test-digestion of some of the Mini-preps (Ori alone, Ori-Amp) --> Run gel
 
-
* Recovered the wildtype Asaia worked!!
 
-
* WT Asaia strains arrived. Culture started
 
-
=27-07-2010=
 
-
*  Ligation C3/A3 + Ori + resistance (Kan, Amp, Tet, Cm)
 
-
* transformation of the ligation products into E.Coli DH5a
 
-
* Plating of transformed cells with selection of the vector and the insert
 
-
* Colony PCR of E.Coli DB3.1 containing BaseVector + Ori + Kan --> running gel (twice, the first time failed)
 
-
* Preculture of (maybe!!) WT Asaia for competence protocol
 
-
* Sorting of male/female flies
 
-
* Culture of 400 ml of different species of bacteria.
 
-
=28-07-2010=
 
-
* Analysis of plating of transformed cells
 
-
* Gel for check the Colony PCR
 
-
* 250 ml Gly medium
 
-
* OD measurement of preculture for competent Asaia --> too high. Start a new one!!!
 
-
* Competent Asaia and stocking @-80°C
 
-
* Plating of transformed E.Coli for double check
 
-
* preparation of the pellets for infection with 3 different species of bacteria + OD measurements
 
-
* preparation of tubes with 15 female flies
 
-
* begin of the survival experiment: infection of flies --> check of the flies after 2h
 
-
=29-07-2010=
 
-
* Results from plating of transformed E.Coli for double check and miniprep from some of them
 
-
* Digestion with different restriction enzymes to check if we get the right length
 
-
* Counting of dead flies one day after the infection
 
-
* Observation of fluorescence of infected flies under the microscope
 
-
=30-07-2010=
 
-
* Running a gel of the digested products of miniprep (29.07) --> bad results
 
-
* Miniprep for the last 17 samples of replating
 
-
* Digestion of 5 samples with 3 restriction enzymes to check if we get the fragments we expect
 
-
* Running a gel for the digestion products
 
-
* Counting of dead flies two days after the infection
 
-
* Observation of fluorescence of infected flies under the microscope
 
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
= How to use the Notebook =
 +
 +
This is a NOTEbook and not a LABbook. Here you can describe what you have done during your exciting day. If you want to have more details you can look in the IRL Labbook. Use ctrl+F to find something ;)
 +
 +
== template of the day ==
 +
<html> <table cellspacing="15" > <tr> <td> </html>
 +
For each new day in the lab you must create a new date in the notebook. To do so, copy-paste the following template:
 +
 +
<pre>
= 02-08-2010 =
= 02-08-2010 =
{| cellspacing="15"
{| cellspacing="15"
| style="width: 33.33%" valign="top" |
| style="width: 33.33%" valign="top" |
== Other ==
== Other ==
-
We make some plate with GLY medium and Agar.
+
It rain a lot today
| style="width: 33.33%" valign="top" |
| style="width: 33.33%" valign="top" |
== Asaia ==
== Asaia ==
-
Today we transform competent Asaia with a GFP + kanamycin (resistance) following this [https://static.igem.org/mediawiki/2010/a/ac/EPFL_prot_Electroporation_asaia.pdf protocols]. We plate it and we'll waiting 2 days to see if it works.
+
Today I speak with an asaia and she answer to me ^^
-
we do digestion to create our first biobrick. We will make the ligation and the transformation in E-Coli tomorrow.
+
| style="width: 33.33%" valign="top" |
| style="width: 33.33%" valign="top" |
== Drosophile ==
== Drosophile ==
-
we count the drosophile and take photos with the fluorecense microscope. In some tubes the filter has detached so the expermiment for those tube are finished.
+
|}
|}
 +
</pre>
 +
 +
<html> </td><td> </html>
 +
 +
<u>Here are some useful explanations : </u>
 +
 +
1. The date format is important!
 +
 +
2. Just after the date, put the following two lines :
 +
<pre>
 +
{| cellspacing="15"
 +
| style="width: 33.33%" valign="top" |
 +
</pre>
 +
3. Beetween each subtitle, put this line :
 +
<pre>
 +
| style="width: 33.33%" valign="top" |
 +
</pre>
 +
4. To end the day just put :
 +
<pre>
 +
|}
 +
</pre>
 +
5. Of course the text "width:33.33%" must be adapted on how many subtitle you have (for instance 4 subtitles = 25%)
 +
 +
<html></td></tr></table></html>
 +
 +
 +
 +
 +
{{EPFL_2010_bottom_wrap}}
</div>
</div>

Latest revision as of 20:02, 27 October 2010
































How to use the Notebook

This is a NOTEbook and not a LABbook. Here you can describe what you have done during your exciting day. If you want to have more details you can look in the IRL Labbook. Use ctrl+F to find something ;)

template of the day

For each new day in the lab you must create a new date in the notebook. To do so, copy-paste the following template: 

= 02-08-2010 =
{| cellspacing="15"
| style="width: 33.33%" valign="top" |
== Other ==
It rain a lot today

| style="width: 33.33%" valign="top" |
== Asaia ==
Today I speak with an asaia and she answer to me ^^

| style="width: 33.33%" valign="top" |
== Drosophile ==
 
|}

Here are some useful explanations :

1. The date format is important!

2. Just after the date, put the following two lines : 

 {| cellspacing="15"
 | style="width: 33.33%" valign="top" |

3. Beetween each subtitle, put this line : 

 | style="width: 33.33%" valign="top" |

4. To end the day just put : 

 |}

5. Of course the text "width:33.33%" must be adapted on how many subtitle you have (for instance 4 subtitles = 25%)



wrap bottom

Retrieved from "http://2010.igem.org/Team:EPF_Lausanne/Notebook"