Team:UNIPV-Pavia/Calendar/July/settimana2

(Difference between revisions)

Revision as of 16:43, 11 July 2010

JULY: WEEK 2

Transformation of 1ul (~4ng) of miniprepped <partinfo>BBa_J23118</partinfo> into 100ul of home-made competent cells

to verify the transformation efficiency.

Transformed bacteria were plated on LB+Amp and left overnight in oven, 37°C.

We checked the presence of colonies in plates. All plates showed red colonies!

MG1655 plate (with satellite colonies)	BW25141 plate
BW25142 plate	BW53474 plate
DH5-alpha plate

We calculated (thanks Nicolò) efficiency as #colonies/ug DNA plated

Strain	#colonies	Efficiency
MG1655	379	~10^5
BW25141	1431	~10^6
BW25142	2374	~10^6
BW53474	9984	~10^7 (very difficult to count correctly)
DH5alpha	7475 (only 100ul of cells plated)	~ 10^7 (very difficult to count correctly, previous tests showed an efficiency of ~10^8)

week 2

week 3

@@ Line 39: / Line 39: @@
 {|align="center"
-| [[Image:MG_unipv10_efficiency.jpg|300px|thumb|center|MG1655 plate]] || [[Image:BW25141_unipv10_efficiency.jpg|300px|thumb|center|BW25141 plate]]
+| [[Image:MG_unipv10_efficiency.jpg|300px|thumb|center|MG1655 plate (with satellite colonies)]] || [[Image:BW25141_unipv10_efficiency.jpg|300px|thumb|center|BW25141 plate]]
 |-
 | [[Image:BW25142_unipv10_efficiency.jpg|300px|thumb|center|BW25142 plate]] || [[Image:BW53474_unipv10_efficiency.jpg|300px|thumb|center|BW53474 plate]]