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Team:TU Delft/Project/alkane-degradation/results
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===[https://2010.igem.org/Team:TU_Delft/Project/alkane-degradation/results/alkane_hydroxylase Characterization of the alkane hydroxylase system]=== | ===[https://2010.igem.org/Team:TU_Delft/Project/alkane-degradation/results/alkane_hydroxylase Characterization of the alkane hydroxylase system]=== | ||
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| + | We've succeeded in characterizing the activity of the alkane hydroxylase system using gas chromatography. By comparing peak ratios we were able to estimate the specific enzymatic activity of the system, which was found to be 0.045 U/mg. For more information about our findings [https://2010.igem.org/Team:TU_Delft/Project/alkane-degradation/results/alkane_hydroxylase CLICK HERE]. | ||
===[https://2010.igem.org/Team:TU_Delft/Project/alkane-degradation/results/LadA Characterization of the long-chain alkane monooxygenase; LadA]=== | ===[https://2010.igem.org/Team:TU_Delft/Project/alkane-degradation/results/LadA Characterization of the long-chain alkane monooxygenase; LadA]=== | ||
Revision as of 19:07, 27 October 2010
Alkane Degradation Results & Conclusions
Characterization of the alkane hydroxylase system
We've succeeded in characterizing the activity of the alkane hydroxylase system using gas chromatography. By comparing peak ratios we were able to estimate the specific enzymatic activity of the system, which was found to be 0.045 U/mg. For more information about our findings CLICK HERE.
Characterization of the long-chain alkane monooxygenase; LadA
Characterization of the Alcohol DeHydrogenase (ADH) system
Characterization of the ALdehyde DeHydrogenase system
USEFUL LITERATURE AND REFERENCES
- Kato T. et al. "Gene cloning and characterization of an aldehyde dehydrogenase from long-chain alkane-degrading Geobacillus thermoleovorans B23" Extremophiles (2010) 14:33-39.
- http://mbel.kaist.ac.kr/lab/research/protein_en1.html
- Hoffmann F. and Rinas U. "Stress Induced by Recombinant Protein Production in Escherichia coli" Advances in Biochemical Engineering/Biotechnology, 2004, Vol. 89/2004, pp. 73-92.
