Team:Osaka/Tests

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<h3>Cellulase assay: CMC clearing</h3>
<h3>Cellulase assay: CMC clearing</h3>
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what are CenA, Cex, PelB, negative controls?
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<table>
<table>
<tr>
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<td><img src="https://static.igem.org/mediawiki/2010/6/61/Cmc1.png" width="200"></td>
<td><img src="https://static.igem.org/mediawiki/2010/6/61/Cmc1.png" width="200"></td>
<td><img src="https://static.igem.org/mediawiki/2010/1/14/Cmc2.png" width="200"></td>
<td><img src="https://static.igem.org/mediawiki/2010/1/14/Cmc2.png" width="200"></td>
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</tr>
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<tr>
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<td>CenA. Clockwise from left: CenA only; CenA with PelB; negative control.</td>
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<td>Cex. Clockwise from top: Cex with PelB; Cex only; negative control.</td>
</tr>
</tr>
</table>
</table>

Revision as of 18:47, 27 October 2010


Tests

Cellulase assay: CMC clearing

what are CenA, Cex, PelB, negative controls?
CenA. Clockwise from left: CenA only; CenA with PelB; negative control. Cex. Clockwise from top: Cex with PelB; Cex only; negative control.

Microscope observation of cell surface display

Cellulase quantitative activity assay: DNS method

Future work

We created many parts (yeast ENO2 promoter, ADH1 terminator, SUC2 secretion signal etc as well as various cellulases such as xylanase and mannase) but did not have time to evaluate them. Also, devices containing 2 or more cellulases should have been constructed and assayed.


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