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Team:Osaka/Tests
From 2010.igem.org
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<h3>Cellulase assay: CMC clearing</h3> | <h3>Cellulase assay: CMC clearing</h3> | ||
| + | what are CenA, Cex, PelB, negative controls? | ||
| + | |||
<table> | <table> | ||
<tr> | <tr> | ||
<td><img src="https://static.igem.org/mediawiki/2010/6/61/Cmc1.png" width="200"></td> | <td><img src="https://static.igem.org/mediawiki/2010/6/61/Cmc1.png" width="200"></td> | ||
<td><img src="https://static.igem.org/mediawiki/2010/1/14/Cmc2.png" width="200"></td> | <td><img src="https://static.igem.org/mediawiki/2010/1/14/Cmc2.png" width="200"></td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td>CenA. Clockwise from left: CenA only; CenA with PelB; negative control.</td> | ||
| + | <td>Cex. Clockwise from top: Cex with PelB; Cex only; negative control.</td> | ||
</tr> | </tr> | ||
</table> | </table> | ||
Revision as of 18:47, 27 October 2010
Tests
Cellulase assay: CMC clearing
what are CenA, Cex, PelB, negative controls? |
 |
| CenA. Clockwise from left: CenA only; CenA with PelB; negative control. | Cex. Clockwise from top: Cex with PelB; Cex only; negative control. |
Microscope observation of cell surface display
Cellulase quantitative activity assay: DNS method
Future work
We created many parts (yeast ENO2 promoter, ADH1 terminator, SUC2 secretion signal etc as well as various cellulases such as xylanase and mannase) but did not have time to evaluate them. Also, devices containing 2 or more cellulases should have been constructed and assayed.
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