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Revision as of 21:04, 23 October 2010 (view source) Anisha (Talk | contribs) (New page: ==August 10th & 11th== *Grew up Lux Promoters (R0061, R0063, K091146, K145150, K091107, R1062) *Made SOB media *Minipreps: - K091107, K091146, R0063, R1062, K145150 - R0061 did not gro...) ← Older edit		Latest revision as of 18:09, 27 October 2010 (view source) Anisha (Talk | contribs)
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	-K3		-K3
	-LP		-LP
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	*Ligations:		*Ligations:
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	- K3 + LP		- K3 + LP
	- K3 + RFP+RBS		- K3 + RFP+RBS
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		+	[https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook]

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Latest revision as of 18:09, 27 October 2010

August 10th & 11th

• Grew up Lux Promoters (R0061, R0063, K091146, K145150, K091107, R1062)
• Made SOB media

• Minipreps:

- K091107, K091146, R0063, R1062, K145150
- R0061 did not grow 
- LP(RFP+RBS) + 2 minipreps of RFP+RBS

• Digests:

- Lux promoters with S+P
- RFP+RBS with E+P and X+P(Phosphatased)
- Digested K3+LP with E+P, K3 was phosphatased

• Gel order:

-Ladder
-K091107
-R0063
-R1062
-K091146
-K145150
-RFP+RBS (EP)
-RFP+RBS (XP)
-K3
-LP

• Ligations:

- RFP+RBS + Lux promoters
- K3 + LP
- K3 + RFP+RBS

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