Team:Calgary/Project/Achievements

From 2010.igem.org

(Difference between revisions)
Line 110: Line 110:
<li>We designed and submitted DNA for three new biobrick parts</li>
<li>We designed and submitted DNA for three new biobrick parts</li>
 +
<ul>
<li><p>Cytoplasmic maltose binding protein (malESS)</li>
<li><p>Cytoplasmic maltose binding protein (malESS)</li>
-
IbpAb/ fxsA fusion promoter
+
<li>IbpAb/ fxsA fusion promoter</li>
-
- cpxP Promoter
+
<li> CpxP Promoter </li></ul>
 +
<ul>
We also built the following new constructs:
We also built the following new constructs:
-
-ibpAB reporter
+
<li>ibpAB reporter</li>
-
CpxR reporter
+
<li>CpxR reporter</li>
-
DegP reporter
+
<li>DegP reporter</li>
-
MalE generator
+
<li>MalE generator</li>
-
MalE31 generator
+
<li>MalE31 generator</li>
-
K13500-I13507-I0500-B0034-malE31
+
<li>K13500-I13507-I0500-B0034-malE31</li></ul>
-
All of these parts were verified via resriction digests and PCR, and then finally sequenced.
+
All of these parts were verified via restriction digests and PCR, and then finally sequenced.
-
Silver medal Requirments
+
<h2 style="color:#0066CC">Silver medal Requirements</h2>
-
Demonstrate that one of your parts works as expected
+
<ul><h3>Demonstrate that one of your parts works as expected</h3>
-
Through our characeroization data, we showed that several of our parts worked as expected.
+
<li>Through our characterization data, we showed that several of our parts worked as expected. Click here for more information.
-
The cpxR reporter was found  to report on  misfolding protein.  We characterized this promoter with varying concentrations of folding and midfolding proteins produced.  We also tested this promoter with NLPE, an outer membrane lipoprotein that literature has found activates the cpx pathway.  Finally, we tested this promoter in varying heat shock conditions.
+
<p>The CpxR reporter was found  to report on  misfolding protein.  We characterized this promoter with varying concentrations of folding and misfolding proteins produced which we assumed is correlated to arabinose concentrations due to the use of AraC promoter.  We also tested this promoter with NlpE, an outer membrane lipoprotein that literature has found activates the cpx pathway.  Finally, we tested this promoter in varying temperature to produce a heat shock response.</p></li>
-
Both MalE and malE31 also worked as expected, malE folding in the periplams and malE31 showing a misfolding reponse.  This was indictaed through tetsing with a reporter from another lab (Raivio) (for more info click here) as well as tetsing with our cpxR reporter click here.
+
<li>Both MalE and MalE31 also worked as expected, malE folding in the periplasm and malE31 showing a misfolding response.  This was indicated through testing with a reporter from another lab (Raivio) (for more info click here) as well as tetsing with our cpxR reporter click here.</li>
-
We characetrized the cpxR reporter, malE31 ibpAB and .  See characterization data here.
+
<li>We characterized the cpxR reporter, malE31, MalE and IbpAB.  See characterization data here.</li></ul>
-
Characterize or improve an existing BioBrick Part or Device and enter this information back on the Registry.
+
<h2 style="color:#0066CC">Gold Medal requirements</h2>
-
-we entered new DNA as well as new sequences for malE and malE31.
+
<ul><h3>Characterize or improve an existing BioBrick Part or Device and enter this information back on the Registry.</h3>
-
- we entered characterization data for the cpxR promoter
+
-
Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.  
+
<li>we entered new DNA as well as new sequences for malE and malE31.</li>
 +
<li>we entered characterization data for the CpxR promoter.</li>
-
-We tetsed out a part for Lethbridge.  We characterized it for possible misfodling in the cytoplasm.  Resuylts can be found here.
+
<h3>Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system. </h3>
-
- We also attended a regional workship as well as a mock Igem event with the Univeristy of Salberta as well as the Univeristy of Lethbridge.  Here we helped each their out with our projects by practicing our presentaitons for each other, giving suggestions, future directions as well as touble shooting
+
<li>We tested out a part for Lethbridge.  We characterized it for possible misfolding in the cytoplasm.  Results can be found here.</li>
-
-Finally our team filled out surveys for the following teams:
+
<li>We also attended a regional workshop as well as a mock iGEM event with the University of Alberta as well as the University of Lethbridge.  Here we helped each their out with our projects by practicing our presentations for each other, giving suggestions, future directions as well as troubleshooting</li>
 +
<li>Finally our team filled out surveys for the following teams:</li>
-
- This year we chose to approach ethjcs by making a pods acts covering a few different ethical issues pertaining to synthetic biology.  We felt tat this would be a novel way to increase awareness about the field of syntyehtic biology while having a discussion of important issues.  We felt that this would be a much more accessible way for the public to gain a better understanding of what the real fears in sythetic biology is as well as to dispell some of the misleading iompressions given by media sources.
+
 
 +
<li>This year we chose to approach ethics by making a podscasts covering a few different ethical issues pertaining to synthetic biology.  We felt tat this would be a novel way to increase awareness about the field of synthetic biology while having a discussion of important issues.  We felt that this would be a much more accessible way for the public to gain a better understanding of what the real fears in synthetic biology is as well as to dispel some of the misleading impressions given by media sources.</li>
 +
</ul>
</p>
</p>
</div>
</div>

Revision as of 16:38, 27 October 2010

Project Descriptions

Achievements

Bronze medal requirements

  • Register the team
  • Successfully complete and submit a project summary form
  • Create a Wiki which includes all the details of the project
  • Present a presentation and a poster at the iGEM Jamboree
  • Enter information detailing at least one new standard BioBrick Part or Device in the Registry of Parts
  • Data was entered for 11 new biobrick parts
  • Submit DNA for at least one new BioBrick Part or Device to the Registry of Parts
  • We designed and submitted DNA for three new biobrick parts

    • Cytoplasmic maltose binding protein (malESS)

    • IbpAb/ fxsA fusion promoter
    • CpxP Promoter
      We also built the following new constructs:
    • ibpAB reporter
    • CpxR reporter
    • DegP reporter
    • MalE generator
    • MalE31 generator
    • K13500-I13507-I0500-B0034-malE31
    All of these parts were verified via restriction digests and PCR, and then finally sequenced.

    Silver medal Requirements

      Demonstrate that one of your parts works as expected

    • Through our characterization data, we showed that several of our parts worked as expected. Click here for more information.

      The CpxR reporter was found to report on misfolding protein. We characterized this promoter with varying concentrations of folding and misfolding proteins produced which we assumed is correlated to arabinose concentrations due to the use of AraC promoter. We also tested this promoter with NlpE, an outer membrane lipoprotein that literature has found activates the cpx pathway. Finally, we tested this promoter in varying temperature to produce a heat shock response.

    • Both MalE and MalE31 also worked as expected, malE folding in the periplasm and malE31 showing a misfolding response. This was indicated through testing with a reporter from another lab (Raivio) (for more info click here) as well as tetsing with our cpxR reporter click here.
    • We characterized the cpxR reporter, malE31, MalE and IbpAB. See characterization data here.

    Gold Medal requirements

      Characterize or improve an existing BioBrick Part or Device and enter this information back on the Registry.

    • we entered new DNA as well as new sequences for malE and malE31.
    • we entered characterization data for the CpxR promoter.

      • Help another iGEM team by, for example, characterizing a part, debugging a construct, or modeling or simulating their system.

    • We tested out a part for Lethbridge. We characterized it for possible misfolding in the cytoplasm. Results can be found here.
    • We also attended a regional workshop as well as a mock iGEM event with the University of Alberta as well as the University of Lethbridge. Here we helped each their out with our projects by practicing our presentations for each other, giving suggestions, future directions as well as troubleshooting
    • Finally our team filled out surveys for the following teams:
    • This year we chose to approach ethics by making a podscasts covering a few different ethical issues pertaining to synthetic biology. We felt tat this would be a novel way to increase awareness about the field of synthetic biology while having a discussion of important issues. We felt that this would be a much more accessible way for the public to gain a better understanding of what the real fears in synthetic biology is as well as to dispel some of the misleading impressions given by media sources.

    Retrieved from "http://2010.igem.org/Team:Calgary/Project/Achievements"