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Team:Slovenia/PROJECT/proof/popfret/split
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| + | <h2>Introduction</h2> | ||
| + | We used split fluorescent proteins fused with zinc fingers to demonstrate the need of a DNA program for fluorescent protein reassembly. To our knowledge, reconstituition of split GFPs using DNA program has never been demonstrated <em>in vivo</em>, but only <em>in vitro </em>(Segal et al., 2005). In addition we wanted to demonstrate that the distance between zinc finger binding sites plays an important role in split GFP reconstitution. Moreover, we tested split GFP (mCerulean and mCitrine) reassembly<em> in vitro</em> using cell lysates. We selected mCitrine, a yellow variant of GFP for the initial experiments of reconstitution of split GFPs. mCitrine is brighter than enhanced yellow GFP and less sensitive to lower pH than EYFP or mVenus. | ||
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| + | [[Image:slo_split_prava.jpg|thumbs|center|650px]] | ||
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Revision as of 16:06, 27 October 2010
Contents |
Introduction
We used split fluorescent proteins fused with zinc fingers to demonstrate the need of a DNA program for fluorescent protein reassembly. To our knowledge, reconstituition of split GFPs using DNA program has never been demonstrated in vivo, but only in vitro (Segal et al., 2005). In addition we wanted to demonstrate that the distance between zinc finger binding sites plays an important role in split GFP reconstitution. Moreover, we tested split GFP (mCerulean and mCitrine) reassembly in vitro using cell lysates. We selected mCitrine, a yellow variant of GFP for the initial experiments of reconstitution of split GFPs. mCitrine is brighter than enhanced yellow GFP and less sensitive to lower pH than EYFP or mVenus.
