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Team:NYMU-Taipei/Experiments
From 2010.igem.org
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= <font color=blue>Experiments</font> = | = <font color=blue>Experiments</font> = | ||
| - | Lab notebooks: | + | Lab notebooks (click the following links to see details): |
* [[Team:NYMU-Taipei/Experiments/Riboswitch|Speedy switch lab notebook]] | * [[Team:NYMU-Taipei/Experiments/Riboswitch|Speedy switch lab notebook]] | ||
* [[mRNA Binding Experiments|Speedy reporter lab notebook]] | * [[mRNA Binding Experiments|Speedy reporter lab notebook]] | ||
Revision as of 15:26, 27 October 2010
| Home | Project Overview | Speedy reporter | Speedy switch | Speedy protein degrader | Experiments and Parts | Applications | F.A.Q | About Us |
Contents |
Experiments
Lab notebooks (click the following links to see details):
Experimental results:
- Speedy switch results: Several reporter gene assay results for testing the Theophylline riboswitch.
- Speedy degrader results: Several reporter gene assay results for testing the half life of various ssrA tagged and untagged fluorescent proteins.
Protocols, preparation and other stuff
- Basic cloning protocols. Optimised protocols developed and kept current over the past few years.
- Gene reporter assay protocols
- pSB1C3 preparation
- dddddH2O preparation (fun :P)
Parts
The range given to each group for this years' parts was divided as follows:
- Speedy Switch: K411000-K411099
- Speedy Reporter: K411100-K411199
- Speedy Protein degrader: K411200-K411299
The designed parts: <groupparts>iGEM010 NYMU-Taipei</groupparts>
Primers
A list of all the primers we designed.
Speedy Reporter
| name | sequence | length | desc |
|---|---|---|---|
| eIF4A_split_A_Fp | cggcagcagcggcagcggcagcATGGAGCCGGAAGGCGTCATCGA | 45 | |
| eIF4A_split_B_Rp | ctgcagcggccgctactagtaTCAAATGAGGTCAGCAACGTTGAG | 45 | |
| GFP_split_A_FP | gaattcgcggccgcttctagagatgcgtaaaggagaagaacttttc | 46 | |
| GFP_split_B_RP | ctgccgctgctgccgctgctgccttattatttgtatagttcatccatgcca | 51 | |
| GFP_split_A_RP | gctgccgctgctgccgctgctgccttgtttgtctgccatgatgtatac | 48 | |
| GFP_split_B_FP | gctgccgctgctgccgctgctgcctttgtatagttcatccatgccatg | 48 | |
| aptamer_FP | gcttctagagacactcggaggacagcttagatgcaaagccggagtgagtgtacacc | 56 | |
| aptamer_RP | agcctgcagcggccgctactagtattcccctggcgcggggtgtacactcactccggct | 58 | |
| eIFA_FP | gcttctagATGGAGCCGGAAGGCGTCATCGA | 31 | |
| eIF4A_mut1_FP | ccatatcaattctccagcagattga | 25 | |
| eIF4A_mut1_RP | caatctgctggagaattgatatggc | 25 | |
| eIF4A_mut2_FP | gcagaagctccagatggaa | 19 | |
| eIF4A_mut2_RP | tccatctggagcttctgca | 19 | |
| eIF4A_split_A_Rp | ctgcagcggccgctactagtatcaagggtctctcataaatttcttgg | 47 | |
| eIF4A_split_B_Fp | cggcagcagcggcagcggcagcattcggattcttgtcaagaagg | 44 | |
| RFP_split_A_FP | gaattcgcggccgcttctagatggcttcctccgaagac | 38 | |
| RFP_split_A_RP | gctgccgctgctgccgctgctgccgtcttccgggtacatacgtt | 44 | |
| RFP_split_B_FP | gaattcgcggccgcttctagatgggtgctctgaaaggtgaaatc | 44 | |
| RFP_split_B_RP | gctgccgctgctgccgctgctgccagcaccggtggagtga | 40 | |
| Total | 773 |
Speedy Switch
| name | sequence | length |
|---|---|---|
| Theophylline Riboswitch FP | gaattcgcggccgcttctagagggtgataccagcatcgtcttgatgcccttggcag | 56 |
| Theophylline Riboswitch RP | ctgcagcggccgctactagtacttgttgtcttgcagcggggtgctgccaagggcatcaagac | 62 |
Speedy Protein Degrader
| name | sequence | length | desc |
|---|---|---|---|
| GFPLVA_out_FP | gcttctagagaaagaggagaaatactagatgcgtaaaggagaagaacttttc | 52 | |
| RFPLVA_out_FP | gcttctagagaaagaggagaaatactagatggct | 34 | |
| RFPLVA_in_RP | CTACTAAAGCGTAGTTTTCGTCGTTTGCAGCagcaccggtggagtga | 47 | |
| SspB_FP | gcttctagatgGATTTGTCACAGCTAACAC | 30 | |
| SspB_RP | ctgcagcggccgctactagtattaCTTCACAACGCGTAATGC | 41 | |
| RFP_FP | gcttctagatggcttcctccgaagac | 26 | |
| GFP_FP | gcttctagatgcgtaaaggagaagaacttttc | 32 | |
| xFP_LVA_Remover_FP | gagctgtacaagaggccttaataatactagagccaggcatca | 42 | |
| xFP_LVA_Remover_RP | tgatgcctggctctagtattattaaggcctcttgtacagctc | 42 | |
| Total | 346 |
