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Revision as of 15:44, 8 July 2010 (view source) Krr533 (Talk | contribs) ← Older edit		Revision as of 19:20, 9 July 2010 (view source) Gcxmatt (Talk | contribs) (→Assembly) Newer edit →
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	===[[Restriction Enzyme Digests]]===		===[[Restriction Enzyme Digests]]===
		+	===[[Plasmid Construction]]===
	===[[3A Assembly]]===		===[[3A Assembly]]===
	===[[Ligations]]===		===[[Ligations]]===

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Revision as of 19:20, 9 July 2010

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Contents 1 DNA 1.1 Prepping DNA from the kit plates 1.2 Quikchange (from primers to colonies!) 1.3 Kit to Stock Plasmid 1.4 Ethanol Precipitation 1.5 New Part Design(PCR) 2 Bacterial Work 2.1 Transformation 2.2 O/N Culture 2.3 Preparation of Competent Cells 2.4 LB Media 2.5 Preparing Plates 2.6 Glycerol Stocks 3 Assembly 3.1 Restriction Enzyme Digests 3.2 Plasmid Construction 3.3 3A Assembly 3.4 Ligations 3.5 Mini Prep 4 Microscopy 4.1 Confocal Microscopy 4.2 Fluorescence Microscopy 5 Reagents 5.1 Reagents 6 Cell Staining 6.1 Rhodamine-Phalloidin/Calcofluor Staining 6.2 LIVE/DEAD® BacLight - Bacterial Viability Kit

DNA

Prepping DNA from the kit plates

Quikchange (from primers to colonies!)

Kit to Stock Plasmid

Ethanol Precipitation

New Part Design(PCR)

Bacterial Work

Transformation

O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

Glycerol Stocks

Assembly

Restriction Enzyme Digests

Plasmid Construction

3A Assembly

Ligations

Mini Prep

Microscopy

Confocal Microscopy

Fluorescence Microscopy

Reagents

Reagents

Cell Staining

Rhodamine-Phalloidin/Calcofluor Staining

LIVE/DEAD® BacLight - Bacterial Viability Kit