Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test2settembre

From 2010.igem.org

(Difference between revisions)
m (RESULTS)
 
(12 intermediate revisions not shown)
Line 11: Line 11:
<td valign="top">
<td valign="top">
<table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px">
<table border="0" align="center" width="100%"><tr><td align="justify" valign="top" style="padding:20px">
-
<html><p align="center"><font size="5"><b>17° TEST</b></font><br><br><font size="4">[SEPTEMBER, 2ND]</font></p></html><hr><br>
+
<html><p align="center"><font size="5"><b>SEPTEMBER, 2ND</b></font></p></html><hr><br>
==PLATE==
==PLATE==
<br>
<br>
Line 18: Line 18:
==EXPERIMENT DESCRIPTION==
==EXPERIMENT DESCRIPTION==
-
==RESULTS (?)==
+
 
 +
===Motivation===
 +
This experiment was performed in M9 to check bacterial growth and RFP rate synthesis in order to evaluate the R.P.U. ([[Team:UNIPV-Pavia/Parts/Characterization#Data analysis for RPU evaluation|see Protocol]]) of the promoters from Anderson's collection in high copy and low copy number plasmids.
 +
 
 +
===Methods===
 +
Accordign to [[Team:UNIPV-Pavia/Parts/Characterization#Microplate reader experiments for constitutive promoters (R.P.U. evaluation) - Protocol #2|Protocol 2]] the experiment was performed in M9 on the following parts:
 +
 
 +
* <partinfo>BBa_J23100</partinfo>
 +
* <partinfo>BBa_J23101</partinfo>
 +
* <partinfo>BBa_J23105</partinfo>
 +
* <partinfo>BBa_J23106</partinfo>
 +
* <partinfo>BBa_J23110</partinfo>
 +
* <partinfo>BBa_J23114</partinfo>
 +
* <partinfo>BBa_J23116</partinfo>
 +
* <partinfo>BBa_J23118</partinfo>
 +
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23100</partinfo> (wiki name: BBa_J23100_4C5)
 +
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23101</partinfo> (wiki name: BBa_J23101_4C5)
 +
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23105</partinfo> (wiki name: BBa_J23105_4C5)
 +
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23106</partinfo> (wiki name: BBa_J23106_4C5)
 +
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23110</partinfo> (wiki name: BBa_J23110_4C5)
 +
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23114</partinfo> (wiki name: BBa_J23114_4C5)
 +
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23116</partinfo> (wiki name: BBa_J23116_4C5)
 +
* <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23118</partinfo> (wiki name: BBa_J23118_4C5)
 +
* <partinfo>BBa_B0031</partinfo> (negative control)
 +
 
 +
==RESULTS==
 +
 
 +
[[Image:UNIPV_Pavia_HCpromotersM9.png| 620px | center ]]
 +
 
 +
In high copy number plasmids it is possible to identify 3 groups: strong promoters (<partinfo>Ba_J23100</partinfo> and <partinfo>BBa_J23101</partinfo>), medium strength promoters (in order <partinfo>BBa_J23110</partinfo> and <partinfo>BBa_J23118</partinfo>) and a group of weak promoters.
 +
 
 +
{| border='1' align='center'
 +
|| Promoter || R.P.U. (average) || std
 +
|-
 +
|| <partinfo>BBa_J23100</partinfo> || 0.946 || 0.182
 +
|-
 +
|| <partinfo>BBa_J23101</partinfo> || 1 || -
 +
|-
 +
|| <partinfo>BBa_J23105</partinfo> || 0.196 || 0.038
 +
|-
 +
|| <partinfo>BBa_J23106</partinfo> || 0.326 || 0.015
 +
|-
 +
|| <partinfo>BBa_J23110</partinfo> || 0.678 || 0.096
 +
|-
 +
|| <partinfo>BBa_J23114</partinfo> || 0.091 || 0.022
 +
|-
 +
|| <partinfo>BBa_J23116</partinfo> || 0.101 || 0.004
 +
|-
 +
|| <partinfo>BBa_J23118</partinfo> || 0.655 || 0.035
 +
|}
 +
 
 +
[[Image:UNIPV_Pavia_LCpromotersM9.png| 620px | center ]]
 +
 
 +
In low copy number plasmids there are the same 3 groups but <partinfo>BBa_J23118</partinfo> resulted stronger than <partinfo>BBa_J23110</partinfo>.
 +
 
 +
{| border='1' align='center'
 +
|| Promoter || R.P.U. (average) || std
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23100</partinfo> || 0.838 || 0.101
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23101</partinfo> || 1 || -
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23105</partinfo> || 0.149 || 0.045
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23106</partinfo> || 0.286 || 0.033
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23110</partinfo> || 0.466 || 0.101
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23114</partinfo> || 0.053 || 0.048
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23116</partinfo> || 0.110 || 0.007
 +
|-
 +
|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23118</partinfo> || 0.714 || 0.154
 +
|}
<!-- table previous next test -->
<!-- table previous next test -->
Line 25: Line 98:
<tr>
<tr>
<td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test1settembre|Previous Test]]</td>
<td align="left" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test1settembre|Previous Test]]</td>
-
<td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test14settembre| Next Test]]</td>
+
<td align="right" width="50%">[[Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test8settembre| Next Test]]</td>
</tr>
</tr>
</table>
</table>
Line 35: Line 108:
<td align="left" valign="top" width="15%">
<td align="left" valign="top" width="15%">
-
<table border="0" width="100%" height="100%">
+
{{UNIPV-Pavia/Menu_measurments}}
-
<tr align="right"><td height="15">
+
-
[[Team:UNIPV-Pavia/Material_Methods/Measurements|MEASUREMENTS]]
+
-
</td></tr>
+
-
<tr align="right"><td height="15">
+
-
[[Team:UNIPV-Pavia/Material_Methods/Measurements/Tecan|Tecan Test]]
+
-
</td></tr>
+
-
</table>
+
-
 
+
</td>
</td>
</table>
</table>

Latest revision as of 14:10, 27 October 2010

SEPTEMBER, 2ND



PLATE


UNIPV Pavia piastra2settembre.png


EXPERIMENT DESCRIPTION

Motivation

This experiment was performed in M9 to check bacterial growth and RFP rate synthesis in order to evaluate the R.P.U. (see Protocol) of the promoters from Anderson's collection in high copy and low copy number plasmids.

Methods

Accordign to Protocol 2 the experiment was performed in M9 on the following parts:

  • <partinfo>BBa_J23100</partinfo>
  • <partinfo>BBa_J23101</partinfo>
  • <partinfo>BBa_J23105</partinfo>
  • <partinfo>BBa_J23106</partinfo>
  • <partinfo>BBa_J23110</partinfo>
  • <partinfo>BBa_J23114</partinfo>
  • <partinfo>BBa_J23116</partinfo>
  • <partinfo>BBa_J23118</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23100</partinfo> (wiki name: BBa_J23100_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23101</partinfo> (wiki name: BBa_J23101_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23105</partinfo> (wiki name: BBa_J23105_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23106</partinfo> (wiki name: BBa_J23106_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23110</partinfo> (wiki name: BBa_J23110_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23114</partinfo> (wiki name: BBa_J23114_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23116</partinfo> (wiki name: BBa_J23116_4C5)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23118</partinfo> (wiki name: BBa_J23118_4C5)
  • <partinfo>BBa_B0031</partinfo> (negative control)

RESULTS

UNIPV Pavia HCpromotersM9.png

In high copy number plasmids it is possible to identify 3 groups: strong promoters (<partinfo>Ba_J23100</partinfo> and <partinfo>BBa_J23101</partinfo>), medium strength promoters (in order <partinfo>BBa_J23110</partinfo> and <partinfo>BBa_J23118</partinfo>) and a group of weak promoters.

Promoter R.P.U. (average) std
<partinfo>BBa_J23100</partinfo> 0.946 0.182
<partinfo>BBa_J23101</partinfo> 1 -
<partinfo>BBa_J23105</partinfo> 0.196 0.038
<partinfo>BBa_J23106</partinfo> 0.326 0.015
<partinfo>BBa_J23110</partinfo> 0.678 0.096
<partinfo>BBa_J23114</partinfo> 0.091 0.022
<partinfo>BBa_J23116</partinfo> 0.101 0.004
<partinfo>BBa_J23118</partinfo> 0.655 0.035
UNIPV Pavia LCpromotersM9.png

In low copy number plasmids there are the same 3 groups but <partinfo>BBa_J23118</partinfo> resulted stronger than <partinfo>BBa_J23110</partinfo>.

Promoter R.P.U. (average) std
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23100</partinfo> 0.838 0.101
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23101</partinfo> 1 -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23105</partinfo> 0.149 0.045
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23106</partinfo> 0.286 0.033
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23110</partinfo> 0.466 0.101
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23114</partinfo> 0.053 0.048
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23116</partinfo> 0.110 0.007
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_J23118</partinfo> 0.714 0.154



Tecan Test