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Team:Kyoto/Notebook3
From 2010.igem.org
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| - | After overnight pre-culture, dilute with supplemented M9 media as OD550 was 0.22, and measure OD550 every 30min. At the point of 30min, we add IPTG and continued to measure OD550. We used test tubes to cultivate E.coli. | + | After overnight pre-culture, dilute with supplemented M9 media as OD550 was 0.22, and measure OD550 every 30min. At the point of 30min, we add IPTG and continued to measure OD550. We used test tubes to cultivate E.coli. We cultivated E.coli in test tubes at 37 degreees. |
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We can observe the cell lysis and this result suggest that the timing of the start of cell lysis is depending on the concentration of IPTG. | We can observe the cell lysis and this result suggest that the timing of the start of cell lysis is depending on the concentration of IPTG. | ||
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| + | Discussion | ||
| + | When the concentration of IPTG is over 0.03mM, cell lysis occurs. | ||
| + | We continued to incubate these cultures overnight. | ||
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| + | ====9/27 Wataru Ken ==== | ||
| + | All of the cultures was over 2.0 of OD550. We caouln't find out the cause of it. | ||
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| + | ====10/4 Ken==== | ||
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Revision as of 12:59, 27 October 2010
LastModified: 2010-10-27
Contents |
Notebook3
Measurement of Lysis Cassette
9/26 Wataru,Ken
After overnight pre-culture, dilute with supplemented M9 media as OD550 was 0.22, and measure OD550 every 30min. At the point of 30min, we add IPTG and continued to measure OD550. We used test tubes to cultivate E.coli. We cultivated E.coli in test tubes at 37 degreees.
1
Discussion We can observe the cell lysis and this result suggest that the timing of the start of cell lysis is depending on the concentration of IPTG.
2
Discussion When the concentration of IPTG is over 0.03mM, cell lysis occurs. We continued to incubate these cultures overnight.
9/27 Wataru Ken
All of the cultures was over 2.0 of OD550. We caouln't find out the cause of it.
