Team:UNIPV-Pavia/Calendar/July/settimana2

(Difference between revisions)

Revision as of 12:57, 9 July 2010

JULY: WEEK 2

Transformation of 1ul (~4ng) of miniprepped <partinfo>BBa_J23118</partinfo> into 100ul of home-made competent cells

to verify the transformation efficiency.

Transformed bacteria were plated on LB+Amp and left overnight in oven, 37°C.

We checked the presence of colonies in plates. All plates showed colonies!

We calculated (thanks Nicolò) efficiency as #colonies/ug DNA plated

Strain	#colonies	Efficiency
MG1655	379	~10^5
BW25141	1431	~10^6
BW25142	2374	~10^6
BW53474	9984	~10^7 (very difficult to count correctly)
DH5alpha	7475 (only 100ul of cells plated)	~ 10^7 (very difficult to count correctly, previous tests showed an efficiency of ~10^8)

week 2

week 3

@@ Line 40: / Line 40: @@
 All plates showed colonies!
-{|
+{|align="center"
-| [[Image:MG_unipv_efficiency.jpg|300px|thumb|center|MG1655 plate]] || [[Image:MG_unipv_efficiency.jpg|300px|thumb|center|BW25141 plate]]
+| [[Image:MG_unipv10_efficiency.jpg|300px|thumb|center|MG1655 plate]] || [[Image:BW25141_unipv10_efficiency.jpg|300px|thumb|center|BW25141 plate]]
 |-
-| [[Image:MG_unipv_efficiency.jpg|300px|thumb|center|BW25142 plate]] || [[Image:MG_unipv_efficiency.jpg|300px|thumb|center|BW53474 plate]]
+| [[Image:BW25142_unipv10_efficiency.jpg|300px|thumb|center|BW25142 plate]] || [[Image:BW53474_unipv10_efficiency.jpg|300px|thumb|center|BW53474 plate]]
 |-
-|[[Image:MG_unipv_efficiency.jpg|300px|thumb|center|DH5-alpha plate]]
+|colspan="2"|[[Image:DH5-alpha_unipv10_efficiency.jpg|300px|thumb|center|DH5-alpha plate]]
+|}
+We calculated (thanks Nicolò) efficiency as #colonies/ug DNA plated
+{|border="1" align="center"
+!Strain || #colonies || Efficiency
+|-
+| MG1655 || 379 || ~10^5
+|-
+| BW25141 || 1431 || ~10^6
+|-
+| BW25142 || 2374 || ~10^6
+|-
+| BW53474 || 9984 || ~10^7<br/>(very difficult to count correctly)
+|-
+| DH5alpha ||  7475<br/>(only 100ul of cells plated)  || ~ 10^7<br/>(very difficult to count correctly,<br/>previous tests showed an efficiency of ~10^8)
 |}