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Team:Kyoto/Notebook3
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===Measurement of Lysis Cassette=== | ===Measurement of Lysis Cassette=== | ||
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| + | After overnight pre-culture, dilute with supplemented M9 media as OD550 was 0.22, and measure OD550 every 30min. At the point of 30min, we add IPTG and continued to measure OD550. We used test tubes to cultivate E.coli. | ||
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| + | 1 | ||
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| + | Discussion | ||
| + | We can observe the cell lysis and this result suggest that the timing of the start of cell lysis is depending on the concentration of IPTG. | ||
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| + | ====9/ | ||
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Revision as of 12:09, 27 October 2010
LastModified: 2010-10-27
Contents |
Notebook3
Measurement of Lysis Cassette
9/26 Wataru,Ken
After overnight pre-culture, dilute with supplemented M9 media as OD550 was 0.22, and measure OD550 every 30min. At the point of 30min, we add IPTG and continued to measure OD550. We used test tubes to cultivate E.coli.
1
Discussion We can observe the cell lysis and this result suggest that the timing of the start of cell lysis is depending on the concentration of IPTG.
====9/
