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Team:Chiba/Plasmid1
From 2010.igem.org
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【Abstract】 T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed. | 【Abstract】 T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed. | ||
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| + | [[Image:Chiba-1.jpg|frame|center|Fig. 1 cloning process]] | ||
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Revision as of 11:55, 27 October 2010
PT7/cI hybrid promoter BBa_K396000
low unregulated, repressed by cI, activated by T7, repression is stronger than activation.
| T7/CI-OR1 hybrid promoter 【Abstract】 T7/CI-OR1 hybrid promoter was designed to be activated by T7 RNA Polymerase and repressed by lambda CI protein. In sequence design, lambda CI operator site 1 (OR1, Part of BBa_R1051) was directly attached to the downstream of T7 promoter sequence (BBa_I719005). This characterization was implemented to validate the promoter function. In results, the activation and repression was actually observed. |
File:Chiba-1.jpg
Fig. 1 cloning process
