"
Team:HokkaidoU Japan/Notebook/September7
From 2010.igem.org
(Difference between revisions)
(→コンピテントセルのPCR) |
|||
| (3 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
{{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/September6|September 6]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/September8|September 8]]</div></div> | {{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/September6|September 6]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/September8|September 8]]</div></div> | ||
| - | + | = Checked concentration of DNA used yesterday for ligation = | |
* 4 ng/uL RFP digested using either H buffer and M buffer | * 4 ng/uL RFP digested using either H buffer and M buffer | ||
* pSB1C3 band was very week | * pSB1C3 band was very week | ||
| - | + | = Ethanol precipitation = | |
| - | In | + | In accordance to Mighty Mix default protocol that for ligation DNA must melted in TE and for concentrating it more Ethanol precipitation was done. |
| - | + | = Colony PCR of competent cells = | |
Previous colony PCR produced strange bands so checked if there were from DNA of competent cell | Previous colony PCR produced strange bands so checked if there were from DNA of competent cell | ||
| - | * Colony PCR didn | + | * Colony PCR didn't produce any bands so those bands had to be an artifact from plasmid used for transformation |
Latest revision as of 11:49, 27 October 2010
since 13 Jul 2010
since 1 Aug 2010
Checked concentration of DNA used yesterday for ligation
- 4 ng/uL RFP digested using either H buffer and M buffer
- pSB1C3 band was very week
Ethanol precipitation
In accordance to Mighty Mix default protocol that for ligation DNA must melted in TE and for concentrating it more Ethanol precipitation was done.
Colony PCR of competent cells
Previous colony PCR produced strange bands so checked if there were from DNA of competent cell
- Colony PCR didn't produce any bands so those bands had to be an artifact from plasmid used for transformation
