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Team:DTU-Denmark/Parts
From 2010.igem.org
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<p align="justify">Gifsy1 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy1 promoters and the Gifsy1 repressor. The promoters are divergent; consisting of pR[Gifsy1] and pRM[Gifsy1]. Downstream of the pRM[Gifsy1] promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy1] and a repressor of the pR[Gifsy1]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in <i>E. Coli</i>.</p> | <p align="justify">Gifsy1 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy1 promoters and the Gifsy1 repressor. The promoters are divergent; consisting of pR[Gifsy1] and pRM[Gifsy1]. Downstream of the pRM[Gifsy1] promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy1] and a repressor of the pR[Gifsy1]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in <i>E. Coli</i>.</p> | ||
Reference | Reference | ||
| - | <p align="justify">BBa_K374009 | + | <p align="justify">BBa_K374009</p> |
<font size="3"><b>Divergent promoters from Gifsy2 phage and Gifsy2 repressor GtgR</b></font><br> | <font size="3"><b>Divergent promoters from Gifsy2 phage and Gifsy2 repressor GtgR</b></font><br> | ||
| + | <p align="justify"><i>This regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GtgR repressor. BBa_K374009 can be combined with XXX hence allowing expression of your inserted BioBrick.</i></p> | ||
| + | </i></p> | ||
<p align="justify">Gifsy2 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy2 promoters and the Gifsy2 repressor. The promoters are divergent; consisting of pR[Gifsy2] and pRM[Gifsy2]. Downstream of the pRM[Gifsy2] promoter is the repressor gtgR. gtgR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy2] and a repressor of the pR[Gifsy2]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in <i>E. Coli</i>.</p> | <p align="justify">Gifsy2 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy2 promoters and the Gifsy2 repressor. The promoters are divergent; consisting of pR[Gifsy2] and pRM[Gifsy2]. Downstream of the pRM[Gifsy2] promoter is the repressor gtgR. gtgR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy2] and a repressor of the pR[Gifsy2]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in <i>E. Coli</i>.</p> | ||
</p> | </p> | ||
Revision as of 09:54, 27 October 2010
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BioBricks submittedBBa_K374008 Divergent promoters from Gifsy1 phage and Gifsy1 repressor GogRThis regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GogR repressor. BBa_K374008 can be combined with BBa_K3740010 hence allowing expression of your inserted BioBrick. Gifsy1 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy1 promoters and the Gifsy1 repressor. The promoters are divergent; consisting of pR[Gifsy1] and pRM[Gifsy1]. Downstream of the pRM[Gifsy1] promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy1] and a repressor of the pR[Gifsy1]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in E. Coli. ReferenceBBa_K374009 Divergent promoters from Gifsy2 phage and Gifsy2 repressor GtgRThis regulatory biobrick can be used in a biological circuit. Other BioBricks can be inserted downstream of the pR promoter, however, no expression will be allowed because of the GtgR repressor. BBa_K374009 can be combined with XXX hence allowing expression of your inserted BioBrick. Gifsy2 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy2 promoters and the Gifsy2 repressor. The promoters are divergent; consisting of pR[Gifsy2] and pRM[Gifsy2]. Downstream of the pRM[Gifsy2] promoter is the repressor gtgR. gtgR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy2] and a repressor of the pR[Gifsy2]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in E. Coli. |
Parts
New for iGEM 2010 is the groupparts tag. This tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
<groupparts>iGEM010 DTU-Denmark</groupparts>
