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Revision as of 20:35, 8 July 2010 (view source) Infekt (Talk | contribs) (→Protocols) ← Older edit		Revision as of 20:39, 8 July 2010 (view source) Infekt (Talk | contribs) (→Protocols) Newer edit →
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	'''Using Lab Equipment'''		'''Using Lab Equipment'''
-	[[Media:Obtaining Deionized Water in the ERB]]	+	[[Media:Obtaining Deionized Water in the ERB|Protocol_for_Obataining_Deionized_Water_in_the_ERB.pdf]]
	[[Media:ERB Spectrophotometer]]		[[Media:ERB Spectrophotometer]]

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Revision as of 20:39, 8 July 2010

General Outline for Biobrick Manipulations

Protocols

Using Lab Equipment

Protocol_for_Obataining_Deionized_Water_in_the_ERB.pdf

Media:ERB Spectrophotometer

Enigeering Research Building Autoclave

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

DNA Manipulation

Transformation-heat shock

This is the procedure we can perform in the ERB

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Heat shock

Transformation-electroporation

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Electroporation

Miniprep

DNA Digest

Running a Gel

DNA Purification

Ligation

• Ginko Bioworks Protocol
• Jeremy Minty Protocol