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Team:Michigan/Protocols
From 2010.igem.org
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'''Using Lab Equipment''' | '''Using Lab Equipment''' | ||
| - | [[Media: | + | [[Media:Obtaining Deionized Water in the ERB]] |
| + | [[Media:ERB Spectrophotometer]] | ||
[[Media:7-6-2010_ERB_Autoclave_Liquid_Sterilization_Protocol.pdf|Enigeering Research Building Autoclave]] | [[Media:7-6-2010_ERB_Autoclave_Liquid_Sterilization_Protocol.pdf|Enigeering Research Building Autoclave]] | ||
Revision as of 20:35, 8 July 2010
General Outline for Biobrick Manipulations
Protocols
Using Lab Equipment
Media:Obtaining Deionized Water in the ERB Media:ERB Spectrophotometer Enigeering Research Building Autoclave
Cell Culture
Making cultures from a -80C freezer stock
P. putida KT2440 antibotic resistance tolerance
DNA Manipulation
This is the procedure we can perform in the ERB
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Heat shock
Transformation-electroporation
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Electroporation
- Ginko Bioworks Protocol
- Jeremy Minty Protocol
