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From 2010.igem.org

Revision as of 18:15, 8 July 2010 (view source) Annlesn (Talk | contribs) ← Older edit		Revision as of 18:17, 8 July 2010 (view source) Annlesn (Talk | contribs) Newer edit →
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	'''DNA Manipulation'''		'''DNA Manipulation'''
-	[[Media:General_Transformation_Protocol.pdf|Transformation-heat shock]]	+	[[Media:Protocol_Heat_Shock_Transformation_2.pdf|Transformation-heat shock]]
	*Getting parts from the 360 well registry plates		*Getting parts from the 360 well registry plates
	*Competent cell preparation		*Competent cell preparation

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Revision as of 18:17, 8 July 2010

General Outline for Biobrick Manipulations

Protocols

Using Lab Equipment

Enigeering Research Building Autoclave

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

DNA Manipulation

Transformation-heat shock

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Heat shock

Transformation-electroporation

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Electroporation

Miniprep

DNA Digest

Running a Gel

DNA Purification

Ligation

• Ginko Bioworks Protocol
• Jeremy Minty Protocol