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Team:Michigan/Protocols
From 2010.igem.org
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'''DNA Manipulation''' | '''DNA Manipulation''' | ||
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| + | [[Media:General_Transformation_Protocol.pdf|Transformation-heat shock]] | ||
| + | *Getting parts from the 360 well registry plates | ||
| + | *Competent cell preparation | ||
| + | *Heat shock | ||
[[Media:General_Transformation_Protocol.pdf|Transformation-electroporation]] | [[Media:General_Transformation_Protocol.pdf|Transformation-electroporation]] | ||
Revision as of 18:15, 8 July 2010
General Outline for Biobrick Manipulations
Protocols
Using Lab Equipment
Enigeering Research Building Autoclave
Cell Culture
Making cultures from a -80C freezer stock
P. putida KT2440 antibotic resistance tolerance
DNA Manipulation
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Heat shock
Transformation-electroporation
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Electroporation
- Ginko Bioworks Protocol
- Jeremy Minty Protocol
