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Team:Mexico-UNAM-CINVESTAV/Notebook/Week Six
From 2010.igem.org
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'''Yesterday's overnight inoculum we did miniprep and double digestion with ''EcoR''I and ''Pst''I''' | '''Yesterday's overnight inoculum we did miniprep and double digestion with ''EcoR''I and ''Pst''I''' | ||
| - | '''To confirm the ligation of PCR 4''' | + | '''To confirm the ligation of PCR 4.''' |
[[Image:PCR4.gif]] | [[Image:PCR4.gif]] | ||
Revision as of 06:24, 27 October 2010
Week #6
11th October - 15 October 2010
Monday
We kept inoculated cells on LB liquid medium at 37ºC overnight, each containing 30ml.
Prepared miniprep for I20260 and E0240, we are going to work on the characterize the promoter
and the cold expresion casstte by RPU's.
Tuesday
Yesterday's overnight inoculum we did miniprep and double digestion with EcoRI and PstI
To confirm the ligation of PCR 4.
