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Team:Berkeley/Project/Other organisms
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| - | Our project focused on the delivery of payloads to the cytoplasms of choanoflagellates, but | + | Our project focused on the delivery of payloads to the cytoplasms of choanoflagellates, but our system's simple, nonspecific design makes it applicable to a host of other eukaryotes. Our design is only limited in three main aspects as to its potential targets: |
* The target organism must be able to naturally phagocytose <i>Escheria Coli</i>. | * The target organism must be able to naturally phagocytose <i>Escheria Coli</i>. | ||
* The target organism's phagocytic vesicles must contain cholesterols susceptible to our [https://2010.igem.org/Team:Berkeley/Project/Vesicle_Buster Vesicle-Buster] device. | * The target organism's phagocytic vesicles must contain cholesterols susceptible to our [https://2010.igem.org/Team:Berkeley/Project/Vesicle_Buster Vesicle-Buster] device. | ||
* The target organism must be able to survive in media that is compatible with our [https://2010.igem.org/Team:Berkeley/Project/Self_Lysis Self-Lysis] device. | * The target organism must be able to survive in media that is compatible with our [https://2010.igem.org/Team:Berkeley/Project/Self_Lysis Self-Lysis] device. | ||
| - | We have started looking at other organisms to deliver payloads to | + | We have started looking at other organisms to deliver payloads to with our payload delivery device. Having screened about a dozen single-celled, freshwater eukaryotes for phagocytosis of <i>E. coli</i> and ability to survive in various concentrations of LB, we are now focusing our efforts on [http://en.wikipedia.org/wiki/Tetrahymena Tetrahymena], [http://en.wikipedia.org/wiki/Blepharisma Blepharisma] and [http://en.wikipedia.org/wiki/Paramecium Paramecia]. We have already determined that these three types of organisms will readily eat <i>E. coli</i> and are capable of surviving in mixtures of their culturing media and LB. The images below show bacteria expressing GFP being ingested by these three organisms. The next step is to include the payload delivery device and start assaying them. |
{| cellpadding="10" cellspacing="10" | {| cellpadding="10" cellspacing="10" | ||
| - | |Tetrahymena | + | |<b>Tetrahymena</b> |
| - | ||[[Image:Terta-Phase.jpg|thumb| | + | ||[[Image:Terta-Phase.jpg|thumb|200px|Tetrahymena: Phase contrast]] |
| - | ||[[Image:Tetra-Phase+GFP.jpg|thumb| | + | ||[[Image:Tetra-Phase+GFP.jpg|thumb|200px|Tetrahymena: Phase contrast and GFP]] |
| - | ||[[Image:Tetra-GFP.jpg|thumb| | + | ||[[Image:Tetra-GFP.jpg|thumb|200px|Tetrahymena: GFP only]] |
| - | | | + | |- |
| - | ||[[Image: | + | |<b>Blepharisma</b> |
| - | ||[[Image: | + | ||[[Image:Blepharisma-Phase.jpg|thumb|200px|Blepharisma: Phase contrast]] |
| - | ||[[Image: | + | ||[[Image:Blepharisma-Phase+GFP.jpg|thumb|200px|Blepharisma: Phase contrast and GFP]] |
| - | |Paramecia | + | ||[[Image:Blepharisma-GFP.jpg|thumb|200px|Blepharisma: GFP only]] |
| - | ||[[Image:Paramecia-Phase.jpg|thumb| | + | |- |
| - | ||[[Image:Paramecia-Phase+GFP.jpg|thumb| | + | |<b>Paramecia</b> |
| - | ||[[Image:Paramecia-GFP.jpg|thumb| | + | ||[[Image:Paramecia-Phase.jpg|thumb|200px|Paramecia: Phase contrast]] |
| + | ||[[Image:Paramecia-Phase+GFP.jpg|thumb|200px|Paramecia: Phase contrast and GFP]] | ||
| + | ||[[Image:Paramecia-GFP.jpg|thumb|200px|Paramecia: GFP only]] | ||
Latest revision as of 06:04, 27 October 2010
- Home
- Project
- Overview
- Motivation
- Other Organisms
- Parts
- Self-Lysis
- Vesicle-Buster
- Payload
- [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2010&group=Berkeley Parts Submitted]
- Results
- Judging
- Clotho
- Human Practices
- Team Resources
- Who We Are
- Notebooks:
- [http://www.openwetware.org/wiki/Berk2010-Daniela Daniela's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Christoph Christoph's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Amy Amy's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Tahoura Tahoura's Notebook]
- [http://www.openwetware.org/wiki/Berk2010-Conor Conor's Notebook]
Our project focused on the delivery of payloads to the cytoplasms of choanoflagellates, but our system's simple, nonspecific design makes it applicable to a host of other eukaryotes. Our design is only limited in three main aspects as to its potential targets:
- The target organism must be able to naturally phagocytose Escheria Coli.
- The target organism's phagocytic vesicles must contain cholesterols susceptible to our Vesicle-Buster device.
- The target organism must be able to survive in media that is compatible with our Self-Lysis device.
We have started looking at other organisms to deliver payloads to with our payload delivery device. Having screened about a dozen single-celled, freshwater eukaryotes for phagocytosis of E. coli and ability to survive in various concentrations of LB, we are now focusing our efforts on [http://en.wikipedia.org/wiki/Tetrahymena Tetrahymena], [http://en.wikipedia.org/wiki/Blepharisma Blepharisma] and [http://en.wikipedia.org/wiki/Paramecium Paramecia]. We have already determined that these three types of organisms will readily eat E. coli and are capable of surviving in mixtures of their culturing media and LB. The images below show bacteria expressing GFP being ingested by these three organisms. The next step is to include the payload delivery device and start assaying them.
| Tetrahymena |  |  |  |
| Blepharisma |  |  |  |
| Paramecia |  |  |  |
