"
Team:Queens-Canada/29 June 2010
From 2010.igem.org
(Difference between revisions)
(New page: <h2>June 29, 2010</h2> {{:Team:Queens-Canada/head}} <h3>Liquid Culture</h3> Thai and Hao '''BBa_I13522'''(Amp) Incubated at 37 °C startin...) |
|||
| (One intermediate revision not shown) | |||
| Line 29: | Line 29: | ||
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3> | <h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3> | ||
Thai | Thai | ||
| - | NpHR, ChR2 | + | |
| + | '''NpHR''', '''ChR2''' | ||
Program run using HIFI with an extension time of 30 s and annealing temperature of 60 °C. | Program run using HIFI with an extension time of 30 s and annealing temperature of 60 °C. | ||
| Line 35: | Line 36: | ||
<h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3> | <h3>[[Team:Queens-Canada/protocols#PCR|PCR]]</h3> | ||
Hao | Hao | ||
| + | |||
'''unc-22 C''', '''unc-22 A''' | '''unc-22 C''', '''unc-22 A''' | ||
| Line 42: | Line 44: | ||
Hao | Hao | ||
| - | '''pIC576''', '''pIC518''', NpHR, ChR2, unc-22 C, unc-22 A | + | '''pIC576''', '''pIC518''', '''NpHR''', '''ChR2''', '''unc-22 C''', '''unc-22 A''' |
Used 0.7 g agarose in 100 mL TBE. Gel run for 40 mins at 100 V. | Used 0.7 g agarose in 100 mL TBE. Gel run for 40 mins at 100 V. | ||
{{:Team:Queens-Canada/foot}} | {{:Team:Queens-Canada/foot}} | ||
Latest revision as of 05:40, 27 October 2010
June 29, 2010
Liquid Culture
Thai and Hao
BBa_I13522(Amp)
Incubated at 37 °C starting at 11:00.
Gel Electrophoresis
Thai and Hao
pMec-7, pRab-7, pSip-1
PCR
Basia and Thai
pIC576-1a, pIC57-1b, pIC518-1a, pIC518-b.
Program run using HIFI with an extension time of 30 s and annealing temperature of 61 °C.
PCR
Yuli and Steve
pSip-1:::eGFP extract, pRab-7:::eGFP extract, BBa_I1408
Program run using HIFI with an extension time of 165 s and annealing temperature of 58 °C.
PCR
Thai
NpHR, ChR2
Program run using HIFI with an extension time of 30 s and annealing temperature of 60 °C.
PCR
Hao
unc-22 C, unc-22 A
Program run using HIFI with an extension time of 32 s and annealing temperature of 58 °C.
Gel Electrophoresis
Hao
pIC576, pIC518, NpHR, ChR2, unc-22 C, unc-22 A
Used 0.7 g agarose in 100 mL TBE. Gel run for 40 mins at 100 V.
