"

From 2010.igem.org

Revision as of 18:20, 6 July 2010 (view source) Bzhang89 (Talk | contribs) (→DNA) ← Older edit		Revision as of 15:44, 8 July 2010 (view source) Krr533 (Talk | contribs) Newer edit →
Line 39:		Line 39:
	===[[Kit to Stock Plasmid]]===		===[[Kit to Stock Plasmid]]===
		+	===[[Ethanol Precipitation]]===
	===[[New Part Design(PCR)]]===		===[[New Part Design(PCR)]]===

---

Revision as of 15:44, 8 July 2010

Home	Brainstorm	Team	Acknowledgements	Project	Side Project	Parts	Notebook	Calendar	Protocol	Safety	Links	References	Media	Contact

Contents 1 DNA 1.1 Prepping DNA from the kit plates 1.2 Quikchange (from primers to colonies!) 1.3 Kit to Stock Plasmid 1.4 Ethanol Precipitation 1.5 New Part Design(PCR) 2 Bacterial Work 2.1 Transformation 2.2 O/N Culture 2.3 Preparation of Competent Cells 2.4 LB Media 2.5 Preparing Plates 2.6 Glycerol Stocks 3 Assembly 3.1 Restriction Enzyme Digests 3.2 3A Assembly 3.3 Ligations 3.4 Mini Prep 4 Microscopy 4.1 Confocal Microscopy 4.2 Fluorescence Microscopy 5 Reagents 5.1 Reagents 6 Cell Staining 6.1 Rhodamine-Phalloidin/Calcofluor Staining 6.2 LIVE/DEAD® BacLight - Bacterial Viability Kit

DNA

Prepping DNA from the kit plates

Quikchange (from primers to colonies!)

Kit to Stock Plasmid

Ethanol Precipitation

New Part Design(PCR)

Bacterial Work

Transformation

O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

Glycerol Stocks

Assembly

Restriction Enzyme Digests

3A Assembly

Ligations

Mini Prep

Microscopy

Confocal Microscopy

Fluorescence Microscopy

Reagents

Reagents

Cell Staining

Rhodamine-Phalloidin/Calcofluor Staining

LIVE/DEAD® BacLight - Bacterial Viability Kit