Team:Michigan/Virus Surface Display
From 2010.igem.org
(Difference between revisions)
(→In the Lab) |
|||
(5 intermediate revisions not shown) | |||
Line 5: | Line 5: | ||
|width="700px" | | |width="700px" | | ||
- | 7/10/2010 | + | '''7/10/2010''' |
13:00 | 13:00 | ||
Line 11: | Line 11: | ||
- | 7/13/2010 | + | '''7/13/2010''' |
9:00 | 9:00 | ||
Line 19: | Line 19: | ||
- | 7/15/2010 | + | '''7/15/2010''' |
4:30 | 4:30 | ||
Line 25: | Line 25: | ||
- | 7/18/2010 | + | '''7/18/2010''' |
5:00 | 5:00 | ||
Line 31: | Line 31: | ||
- | 7/21/2010 | + | '''7/21/2010''' |
6:00 | 6:00 | ||
Line 37: | Line 37: | ||
- | 7/23/2010 | + | '''7/23/2010''' |
9:00 | 9:00 | ||
Line 64: | Line 64: | ||
- | 7/25/2010 | + | '''7/25/2010''' |
5:00 | 5:00 | ||
• aliquot INP samples and primers for sequencing | • aliquot INP samples and primers for sequencing | ||
- | 7/26/2010 | + | |
+ | '''7/26/2010''' | ||
• Electroporation transformation of the following Biobrick parts: | • Electroporation transformation of the following Biobrick parts: | ||
- | pBad/araC (I0500) | + | 1. pBad/araC (I0500) |
- | Linker (K157013) | + | 2. Linker (K157013) |
- | GFP (E0040) | + | 3. GFP (E0040) |
- | 7/27/2010 | + | '''7/27/2010''' |
9:00 | 9:00 | ||
Line 83: | Line 84: | ||
- | 7/28/2010 | + | '''7/28/2010''' |
4:00 | 4:00 | ||
Line 92: | Line 93: | ||
[[Image:7-28biobrickgel.jpg|300px]] | [[Image:7-28biobrickgel.jpg|300px]] | ||
- | Lane 1 (far right): | + | Lane 1 (far right): Invitrogen 1 kb Plus ladder |
Lane 2: GFP cut with XbaI and PstI | Lane 2: GFP cut with XbaI and PstI | ||
Line 119: | Line 120: | ||
- | 7/30/2010 | + | '''7/30/2010''' |
10:00 | 10:00 | ||
Line 128: | Line 129: | ||
• Heat shock transformation of ligation | • Heat shock transformation of ligation | ||
- | 8/2/2010 | + | |
+ | '''8/2/2010''' | ||
• Miniprep of INP-linker | • Miniprep of INP-linker | ||
• Nanodrop results: | • Nanodrop results: | ||
Line 137: | Line 139: | ||
2.2 69.8 2.02 2.11 | 2.2 69.8 2.02 2.11 | ||
- | 8/3/2010 | + | '''8/3/2010''' |
• EcoRI+SpeI and SpeI+PstI digests of INP + linker minipreps | • EcoRI+SpeI and SpeI+PstI digests of INP + linker minipreps | ||
Line 167: | Line 169: | ||
Lane 11 - INP 2 (SpeI+PstI) | Lane 11 - INP 2 (SpeI+PstI) | ||
- | |||
- | + | '''8/5/2010''' | |
- | 8/6/2010 | + | • Inoculated 6 ml DH5α for ligation and transformation. |
+ | |||
+ | |||
+ | '''8/6/2010''' | ||
• OmpA and GFP Ligations done: | • OmpA and GFP Ligations done: | ||
Line 179: | Line 183: | ||
• Heat shock transformation | • Heat shock transformation | ||
- | 8/9/2010 | + | |
+ | '''8/9/2010''' | ||
• Miniprep of 8/6 transformation, in addition to pBAD promoter and linker. | • Miniprep of 8/6 transformation, in addition to pBAD promoter and linker. | ||
Line 204: | Line 209: | ||
- | 8/10/10 | + | '''8/10/10''' |
+ | |||
• Gel of 8/9 digest results | • Gel of 8/9 digest results | ||
- | [[Image:081010gel.jpg| | + | [[Image:081010gel.jpg|350px]] |
+ | |||
+ | |||
+ | '''8/12/2010''' | ||
+ | |||
+ | • Due to poor digest results, we repeated the miniprep from 8/9, with additional colonies of OmpA+GFP picked. Also miniprepped are 2 expression plasmids (Backbone and Promoter) transformed earlier by another group. | ||
+ | |||
+ | Sample | ||
+ | |||
Line 223: | Line 237: | ||
[[Image:Virus05.jpg|middle|250px]] | [[Image:Virus05.jpg|middle|250px]] | ||
+ | |||
+ | [[Image:Virus06.jpg|middle|250px]] | ||
+ | |||
+ | [[Image:Virus07.jpg|middle|250px]] | ||
+ | |||
+ | [[Image:Virus08.jpg|middle|250px]] | ||
+ | |||
+ | [[Image:Virus09.jpg|middle|250px]] | ||
+ | |||
+ | [[Image:Virus10.jpg|middle|250px]] | ||
+ | |||
+ | [[Image:Virus11.jpg|middle|250px]] | ||
+ | |||
+ | [[Image:Virus12.jpg|middle|250px]] | ||
+ | |||
+ | [[Image:Virus13.jpg|middle|250px]] | ||
+ | |||
+ | [[Image:Virus14.jpg|middle|250px]] | ||
+ | |||
|} | |} |
Latest revision as of 04:44, 27 October 2010