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| | ==='''July 20'''=== | | ==='''July 20'''=== |
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| - | Activities: | + | |
| - | BioBrick extraction from the distribution plates | + | == Activities:BioBrick extraction from the distribution plates == |
| | + | |
| | | | |
| | '''Methodology Steps:''' | | '''Methodology Steps:''' |
| | | | |
| - | '''1.Locate the BioBrick parts that we are going to assemble, in the distribution plates.''' | + | '''1.Locate the BioBrick parts that we are going to assemble in the distribution plates.''' |
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| | RBS: 2M, GFP: 14K, Terminator: 13D & Promoter: 15N | | RBS: 2M, GFP: 14K, Terminator: 13D & Promoter: 15N |
| | | | |
| - | For that we take the DNA from each of the wells of the plate. We then have to take 2ul of each one and put it in the bacteria. The other 8ul we are to store at -20°C.
| |
| | | | |
| - | '''2.Clone the chosen BioBrick parts in competent ''E. coli''.''' | + | '''2. Resuspend the plasmid part with 10ul water (Nuclease free, distilled)''' |
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| - | For the extraction we follow the comercial protocol. We transformed 4 different bacteria, for the different BioBrick plasmids & prepared LB-ampi. [[Image:Cuadro_medio1.JPG|200px|thumb|left|alt text]] [[Image:Plates2.JPG|400px|thumb|left|alt text]] | + | For that we take the DNA from each of the wells of the plate. We used 2ul of each part to performed the E. coli transformation. The remaining 8ul were store at -20°C. |
| | | | |
| | | | |
| | + | '''3.Transformed the ''E. coli'' with the chosen BioBrick parts.''' |
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| - | Incubate at 37°C overnight
| + | For the extraction we follow the comercial protocol. We transformed 4 different bacteria, for the different BioBrick plasmids. |
| - | LB agar plates were prepared with Ampicillin, pH 7.5
| + | |
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| | + | [[Image:Cuadro_medio1.JPG|200px|thumb|left|alt text]] [[Image:Plates2.JPG|400px|thumb|left|alt text]] |
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| | + | Incubate at 37°C overnight |
| | + | LB agar plates were prepared with Ampicillin, pH 7.5 |
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| - | | + | For the ampicilin we made a frozen stock solution with a concentration of 50ug/ml in water. In the plate we used a concentration of 100mg/ml. |
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| - | For the ampicilin we have to make a frozen stock solution with a concentration of 50ug/ml in water. The plate concentration is of 100mg/ml. | + | |
| - | We want to make a stock solution of 2ml so, we need 100mg of ampicilin to make 50mg/ml. We have to filter this through a 0.2um filter.
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| | Image:Panama-21-7-10-01.JPG| | | Image:Panama-21-7-10-01.JPG| |
| | Image:Panama-21-7-10-02.JPG| | | Image:Panama-21-7-10-02.JPG| |
| - | Image:Panama-21-7-10-03.JPG|
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| - | Image:Panama-21-7-10-05.JPG|
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| - | Image:Panama-21-7-10-06.JPG|
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| - | Image:Panama-21-7-10-07.JPG|
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| - | Image:Panama-21-7-10-08.JPG|
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| - | Image:DSC04818.JPG|
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| - |
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| | </gallery> | | </gallery> |
| | </center> | | </center> |
| | | | |
| | {{:Team:Panama/calendar2}} | | {{:Team:Panama/calendar2}} |
July 20
Methodology Steps:
1.Locate the BioBrick parts that we are going to assemble in the distribution plates.
RBS: 2M, GFP: 14K, Terminator: 13D & Promoter: 15N
2. Resuspend the plasmid part with 10ul water (Nuclease free, distilled)
For that we take the DNA from each of the wells of the plate. We used 2ul of each part to performed the E. coli transformation. The remaining 8ul were store at -20°C.
3.Transformed the E. coli with the chosen BioBrick parts.
For the extraction we follow the comercial protocol. We transformed 4 different bacteria, for the different BioBrick plasmids.
Incubate at 37°C overnight
LB agar plates were prepared with Ampicillin, pH 7.5
For the ampicilin we made a frozen stock solution with a concentration of 50ug/ml in water. In the plate we used a concentration of 100mg/ml.
| May
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/1_May_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/2_May_2010&action=edit 2]
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/3_May_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/4_May_2010&action=edit 4]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/5_May_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/6_May_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/7_May_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/8_May_2010&action=edit 8]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/9_May_2010&action=edit 9]
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/10_May_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/11_May_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/12_May_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/13_May_2010&action=edit 13]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/14_May_2010&action=edit 14]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/15_May_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/16_May_2010&action=edit 16]
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/19_May_2010&action=edit 19]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/20_May_2010&action=edit 20]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/21_May_2010&action=edit 21]
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| [http://2010.igem.org/Team:Panama/26_May_2010 26]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/27_May_2010&action=edit 27]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/28_May_2010&action=edit 28]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/29_May_2010&action=edit 29]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/30_May_2010&action=edit 30]
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/31_May_2010&action=edit 31]
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| June
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/1_June_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/2_June_2010&action=edit 2]
| [http://2010.igem.org/Team:Panama/3_June_2010 3]
| [http://2010.igem.org/Team:Panama/4_June_2010 4]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/5_June_2010&action=edit 5]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/6_June_2010&action=edit 6]
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| [http://2010.igem.org/Team:Panama/7_June_2010 7]
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| [http://2010.igem.org/Team:Panama/9_June_2010 9]
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| [http://2010.igem.org/Team:Panama/11_June_2010 11]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/12_June_2010&action=edit 12]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/13_June_2010&action=edit 13]
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/15_June_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/16_June_2010&action=edit 16]
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/18_June_2010&action=edit 18]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/19_June_2010&action=edit 19]
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/29_June_2010&action=edit 29]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/30_June_2010&action=edit 30]
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| July
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/1_July_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/2_July_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/3_July_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/4_July_2010&action=edit 4]
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/6_July_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/7_July_2010&action=edit 7]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/8_July_2010&action=edit 8]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/9_July_2010&action=edit 9]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/10_July_2010&action=edit 10]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/11_July_2010&action=edit 11]
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/13_July_2010&action=edit 13]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/14_July_2010&action=edit 14]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/15_July_2010&action=edit 15]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/16_July_2010&action=edit 16]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/17_July_2010&action=edit 17]
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| [http://2010.igem.org/Team:Panama/22_July_2010 22]
| [http://2010.igem.org/Team:Panama/23_July_2010 23]
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/25_July_2010&action=edit 25]
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| [http://2010.igem.org/Team:Panama/27_July_2010 27]
| [http://2010.igem.org/Team:Panama/28_July_2010 28]
| [http://2010.igem.org/Team:Panama/29_July_2010 29]
| [http://2010.igem.org/Team:Panama/30_July_2010 30]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/31_July_2010&action=edit 31]
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/1_August_2010&action=edit 1]
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/3_August_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/4_August_2010&action=edit 4]
| [http://2010.igem.org/Team:Panama/5_August_2010 5]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/6_August_2010&action=edit 6]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/7_August_2010&action=edit 7]
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| [http://2010.igem.org/Team:Panama/12_August_2010 12]
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/1_September_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/2_September_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/3_September_2010&action=edit 3]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/4_September_2010&action=edit 4]
| [http://2010.igem.org/Team:Panama/5_September_2010 5]
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| [http://2010.igem.org/Team:Panama/8_September_2010 8]
| [http://2010.igem.org/Team:Panama/9_September_2010 9]
| [http://2010.igem.org/Team:Panama/10_September_2010 10]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/11_September_2010&action=edit 11]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/12_September_2010&action=edit 12]
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| [http://2010.igem.org/Team:Panama/15_September_2010 15]
| [http://2010.igem.org/Team:Panama/16_September_2010 16]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/17_September_2010&action=edit 17]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/18_September_2010&action=edit 18]
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| [http://2010.igem.org/Team:Panama/22_September_2010 22]
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/26_September_2010&action=edit 26]
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| [http://2010.igem.org/Team:Panama/28_September_2010 28]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/29_September_2010&action=edit 29]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/30_September_2010&action=edit 30]
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/1_October_2010&action=edit 1]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/2_October_2010&action=edit 2]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/3_October_2010&action=edit 3]
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/5_October_2010&action=edit 5]
| [http://2010.igem.org/Team:Panama/6_October_2010 6]
| [http://2010.igem.org/Team:Panama/7_October_2010 7]
| [http://2010.igem.org/Team:Panama/8_October_2010 8]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/9_October_2010&action=edit 9]
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| [http://2010.igem.org/Team:Panama/13_October_2010 13]
| [http://2010.igem.org/Team:Panama/14_October_2010 14]
| [http://2010.igem.org/Team:Panama/15_October_2010 15]
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| [http://2010.igem.org/Team:Panama/26_October_2010 26]
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| [http://2010.igem.org/wiki/index.php?title=Team:Panama/29_October_2010&action=edit 29]
| [http://2010.igem.org/wiki/index.php?title=Team:Panama/30_October_2010&action=edit 30]
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