Team:Caltech/Parts
From 2010.igem.org
(Updated BioBrick(tm) list.) |
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Content= | Content= | ||
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- | == | + | == Favorite Bricks == |
Heat shock promoter: [[BBa_K338001]] | Heat shock promoter: [[BBa_K338001]] | ||
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Plastic, part 2: [[Team:Caltech/BBa_K338004|BBa_K338004]] | Plastic, part 2: [[Team:Caltech/BBa_K338004|BBa_K338004]] | ||
- | == | + | == All Bricks== |
<!-- Following code generated by CompletedPartsFormatter, (c) 2010 Alexandre Boulgakov --> | <!-- Following code generated by CompletedPartsFormatter, (c) 2010 Alexandre Boulgakov --> | ||
- | {{{!}} | + | {{{!}} border="1" cellpadding="5" cellspacing="0" |
! scope="col" {{!}} Part Name | ! scope="col" {{!}} Part Name | ||
! scope="col" {{!}} Brick Number | ! scope="col" {{!}} Brick Number | ||
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! scope="col" {{!}} Subparts | ! scope="col" {{!}} Subparts | ||
{{!}}- | {{!}}- | ||
- | {{!}} phaB1.RBS (plastic intermediate) {{!}}{{!}} | + | {{!}} phaB1.RBS (plastic intermediate) {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K332023 BBa_K332023] {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156013 BBa_K156013], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034] |
{{!}}- | {{!}}- | ||
- | {{!}} Heat-Shock Activated Promoter {{!}}{{!}} | + | {{!}} Heat-Shock Activated Promoter {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001] {{!}}{{!}} Amp {{!}}{{!}} Original part |
{{!}}- | {{!}}- | ||
- | {{!}} Heat-Shock or IPTG Induced Generator {{!}}{{!}} | + | {{!}} Heat-Shock or IPTG Induced Generator {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338002 BBa_K338002] {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034] |
{{!}}- | {{!}}- | ||
- | {{!}} pLacI.RBS.phaA.RBS (plastic intermediate) {{!}}{{!}} | + | {{!}} pLacI.RBS.phaA.RBS (plastic intermediate) {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338003 BBa_K338003] {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156012 BBa_K156012], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034] |
{{!}}- | {{!}}- | ||
- | {{!}} phaB1.RBS.phaC1.TT (plastic intermediate) {{!}}{{!}} | + | {{!}} phaB1.RBS.phaC1.TT (plastic intermediate) {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338004 BBa_K338004] {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156013 BBa_K156013], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156014 BBa_K156014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] |
{{!}}- | {{!}}- | ||
- | {{!}} phaA.RBS (plastic intermediate) {{!}}{{!}} | + | {{!}} phaA.RBS (plastic intermediate) {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338022 BBa_K338022] {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156012 BBa_K156012], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034] |
{{!}}- | {{!}}- | ||
- | {{!}} phaC1 with Double Terminator {{!}}{{!}} | + | {{!}} phaC1 with Double Terminator {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338024 BBa_K338024] {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K156014 BBa_K156014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] |
{{!}}- | {{!}}- | ||
- | {{!}} +OmpR, +(CinR-HSL) Double Promoter {{!}}{{!}} | + | {{!}} +OmpR, +(CinR-HSL) Double Promoter {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338029 BBa_K338029] {{!}}{{!}} Tet {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077] |
{{!}}- | {{!}}- | ||
- | {{!}} GFP Reporter Device, Heat-Shock Induced {{!}}{{!}} | + | {{!}} GFP Reporter Device, Heat-Shock Induced {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338042 BBa_K338042] {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] |
{{!}}- | {{!}}- | ||
- | {{!}} +Heat-Shock, +CinR-HSL Double Promoter {{!}}{{!}} | + | {{!}} +Heat-Shock, +CinR-HSL Double Promoter {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338046 BBa_K338046] {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077] |
{{!}}- | {{!}}- | ||
- | {{!}} GFP Reporter Device, Heat-Shock or IPTG Induced {{!}}{{!}} | + | {{!}} GFP Reporter Device, Heat-Shock or IPTG Induced {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338062 BBa_K338062] {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] |
{{!}}- | {{!}}- | ||
- | {{!}} Heat-Shock Activated Generator {{!}}{{!}} | + | {{!}} Heat-Shock Activated Generator {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338081 BBa_K338081] {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034] |
{{!}}- | {{!}}- | ||
- | {{!}} GFP Reporter Device, Cinr-HSL Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} | + | {{!}} GFP Reporter Device, Cinr-HSL Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] |
{{!}}- | {{!}}- | ||
- | {{!}} HSL with Double Terminator {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} | + | {{!}} HSL with Double Terminator {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] |
{{!}}- | {{!}}- | ||
- | {{!}} Constitutive Generator {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} | + | {{!}} Constitutive Generator {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 BBa_J23119], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034] |
{{!}}- | {{!}}- | ||
- | {{!}} CinR with Double Terminator {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} | + | {{!}} CinR with Double Terminator {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 BBa_C0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] |
{{!}}- | {{!}}- | ||
- | {{!}} +IPTG, +(CinR-HSL) Double Promoter {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} | + | {{!}} +IPTG, +(CinR-HSL) Double Promoter {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077] |
{{!}}- | {{!}}- | ||
- | {{!}} OmpR Induced Generator {{!}}{{!}} Not Submitted {{!}}{{!}} Tet {{!}}{{!}} | + | {{!}} OmpR Induced Generator {{!}}{{!}} Not Submitted {{!}}{{!}} Tet {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034] |
{{!}}- | {{!}}- | ||
- | {{!}} HSL.TT.pLacI.p(CinR-HSL) (intermediate) {{!}}{{!}} Not Submitted {{!}}{{!}} Amp {{!}}{{!}} | + | {{!}} HSL.TT.pLacI.p(CinR-HSL) (intermediate) {{!}}{{!}} Not Submitted {{!}}{{!}} Amp {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0011 BBa_R0011], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077] |
{{!}}- | {{!}}- | ||
- | {{!}} HSL Device, CinR-HSL Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} | + | {{!}} HSL Device, CinR-HSL Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] |
{{!}}- | {{!}}- | ||
- | {{!}} HSL Device {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} | + | {{!}} HSL Device {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0076 BBa_C0076], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] |
{{!}}- | {{!}}- | ||
- | {{!}} Heat-Shock Activated Generator {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} | + | {{!}} Heat-Shock Activated Generator {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K338001 BBa_K338001], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034] |
{{!}}- | {{!}}- | ||
- | {{!}} GFP Reporter Device, OmpR Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} | + | {{!}} GFP Reporter Device, OmpR Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] |
{{!}}- | {{!}}- | ||
- | {{!}} GFP Reporter Device, Constitutive {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} | + | {{!}} GFP Reporter Device, Constitutive {{!}}{{!}} Not Submitted {{!}}{{!}} Kan {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 BBa_J23119], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_E0040 BBa_E0040], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] |
{{!}}- | {{!}}- | ||
- | {{!}} K124014 Holin with Double Terminator {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} | + | {{!}} K124014 Holin with Double Terminator {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124014 BBa_K124014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] |
{{!}}- | {{!}}- | ||
- | {{!}} K124017 Lysis Cassette with Double Terminator {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} | + | {{!}} K124017 Lysis Cassette with Double Terminator {{!}}{{!}} Not Submitted {{!}}{{!}} Cm {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124017 BBa_K124017], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] |
{{!}}- | {{!}}- | ||
- | {{!}} CinR Device, OmpR or CinR-HSL Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Amp {{!}}{{!}} | + | {{!}} CinR Device, OmpR or CinR-HSL Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Amp {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0077 BBa_R0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 BBa_C0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] |
{{!}}- | {{!}}- | ||
- | {{!}} CinR Device, OmpR Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Amp {{!}}{{!}} | + | {{!}} CinR Device, OmpR Induced {{!}}{{!}} Not Submitted {{!}}{{!}} Amp {{!}}{{!}} [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 BBa_R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 BBa_B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_C0077 BBa_C0077], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 BBa_B0015] |
{{!}}} | {{!}}} | ||
<!-- End CompletedPartsFormatter section. --> | <!-- End CompletedPartsFormatter section. --> |
Revision as of 02:29, 27 October 2010
People
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Favorite BricksHeat shock promoter: BBa_K338001 HSP-LacI: BBa_K338002 Plastic, part 1: BBa_K338003 Plastic, part 2: BBa_K338004 All Bricks
ApparatusThe red/infrared light emitting apparatus we built to test our heat shock promoter was modeled after the original [http://openwetware.org/wiki/LightCannon Light Cannon]. It features a theater lamp with a Roscolux #22 Deep Amber red/infrared light filter ([http://www.amazon.com/Rosco-Laboratories-22-ROSCO-Roscolux-22/dp/B000XRCGDE/ref=sr_1_1?ie=UTF8&s=electronics&qid=1287956720&sr=8-1 Amazon], [http://www.rosco.com/includes/filters/SED.asp?titleName=R22:%20Deep%20Amber&imageName=../../images/filters/roscolux/22.jpg Spectrum Data]). We placed this light on the bench top above a rudimentary stage. By varying the height of the lamp above the stage, we could vary the temperature and light intensity at the stage. In the image, the stage temperature was measured to be 42°C.
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