Team:Michigan/Project
From 2010.igem.org
(Difference between revisions)
Chillywings (Talk | contribs) (→Oil Sands: NanR overexpression) |
|||
Line 29: | Line 29: | ||
===Results to Date=== | ===Results to Date=== | ||
We were able to put FimB onto pBAD, an arabinose inducible plasmid. This should have resulted in hyperpiliated E. coli, however we were not able to find many pili on our cells when we used an electron microscope. This is discouraging, but it's not a strong indication of failure because our growth conditions may not have been ideal for pili formation. More tests should be done to determine the optimal pili growth conditions. | We were able to put FimB onto pBAD, an arabinose inducible plasmid. This should have resulted in hyperpiliated E. coli, however we were not able to find many pili on our cells when we used an electron microscope. This is discouraging, but it's not a strong indication of failure because our growth conditions may not have been ideal for pili formation. More tests should be done to determine the optimal pili growth conditions. | ||
- | [[Image: OD_assay_all_samples.png | thumb | 500px|The results of an assay done on 10/19. | + | [[Image: OD_assay_all_samples.png | thumb | 500px|The results of an assay done on [[Team:Michigan/Pili_October#10/19/2010|10/19]]. ]] |
After conducting several assays to determine the effect of hyperpiliated E. coli on the flocculation of algae, we were not able to see very significant results. Hyperpiliated E. coli seem to flocculate the algae faster than nonpiliated E. coli, but the algae seemed to flocculate by itself. This is possibly due to contaminants in the algae. The algae was not grown in sterile conditions. | After conducting several assays to determine the effect of hyperpiliated E. coli on the flocculation of algae, we were not able to see very significant results. Hyperpiliated E. coli seem to flocculate the algae faster than nonpiliated E. coli, but the algae seemed to flocculate by itself. This is possibly due to contaminants in the algae. The algae was not grown in sterile conditions. |
Revision as of 02:21, 27 October 2010