Team:Baltimore US/Team

From 2010.igem.org

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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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Title: DIY-GEM: a path towards low cost high throughput gene synthesis. <br>
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Synthetic biology research requires more cost effective approaches <br>
 +
toward reagents and hardware accessibility.  We are developing <br>
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low-cost alternatives to existing hardware and enzymes in an <br>
 +
attempt to expand participation in biological research and <br>
 +
development.Our project expands the accessibility of Taq Polymerase by <br>
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engineering it in a form compatible with BioBrick assembly.<br>
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This allows use of the over-expressed enzyme from a crude<br>
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bacterial extract in a PCR reaction at a fraction of the cost<br>
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of highly purified commercial enzyme.  In addition, we have<br>
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developed inexpensive and easily assembled lab equipment such<br>
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as a gel electrophoresis apparatus and a PCR thermal cycler.<br> 
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Enabling researchers to synthesize their own enzymes and having<br>
 +
access to inexpensive tools will allow for increased participation<br>
 +
among the DIY-bio community, stretch increasingly scarce educational<br>
 +
funds, and allow rapid scale up of large scale gene synthesis projects.<br>
 +
 +
 +
 +
 +
|[[Image:Baltimore_US_team.png|200 px]]
|[[Image:Baltimore_US_team.png|200 px]]
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Revision as of 01:24, 27 October 2010

You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing. I'd like to have a small paragraph or blurb from the members put together here. with perhaps a caption.

Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)

Title: DIY-GEM: a path towards low cost high throughput gene synthesis.

Synthetic biology research requires more cost effective approaches
toward reagents and hardware accessibility. We are developing
low-cost alternatives to existing hardware and enzymes in an
attempt to expand participation in biological research and
development.Our project expands the accessibility of Taq Polymerase by
engineering it in a form compatible with BioBrick assembly.
This allows use of the over-expressed enzyme from a crude
bacterial extract in a PCR reaction at a fraction of the cost
of highly purified commercial enzyme. In addition, we have
developed inexpensive and easily assembled lab equipment such
as a gel electrophoresis apparatus and a PCR thermal cycler.
Enabling researchers to synthesize their own enzymes and having
access to inexpensive tools will allow for increased participation
among the DIY-bio community, stretch increasingly scarce educational
funds, and allow rapid scale up of large scale gene synthesis projects.



Baltimore US team.png
Team Example


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Meeting/Lab Times This Week Safety


Who we are



Advisors:

  • Lab Manager/Advisor: Thomas Burkett
  • Advisor 1: Lisa Scheifele
  • Advisor 2: T Elizabeth Goode


Undergrads:

  • Student 1: Ryan Ogle, LMT
  • Student 2: Patrick O'Neil
  • Student 3: Robert Buck
  • Student 4: Miles Pekala
  • Student 5: Gary Cygiel
  • Student 6: Steven Stowell
  • Student 7: Duke Kessler
  • Student 8: Bernadette Gallagher


What we did

(Provide proper attribution for all work)


Where we're from