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Team:Northwestern/Protocol
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<!--- The Mission, Experiments ---> | <!--- The Mission, Experiments ---> | ||
| - | {|- | + | {|- style="font-size: 87%; background: #EFCDF8; color: #FFFFFF" cellpadding="1" cellspacing="1" bordercolor="#000000" width="75%" align="center" |
| - | !align="center"|[[Team:Northwestern|< | + | !align="center"|[[Team:Northwestern|<font color="#2B3856">'''Home'''</font>]] |
| - | !align="center"|[[Team:Northwestern/Brainstorm|< | + | !align="center"|[[Team:Northwestern/Brainstorm|<font color="#000000">'''Brainstorm'''</font>]] |
| - | !align="center"|[[Team:Northwestern/Team|< | + | !align="center"|[[Team:Northwestern/Team|<font color="#000000">'''Team'''</font>]] |
| - | !align="center"|[[Team:Northwestern/Acknowledgements|< | + | !align="center"|[[Team:Northwestern/Acknowledgements|<font color="#000000">'''Acknowledgements'''</font>]] |
| - | !align="center"|[[Team:Northwestern/Project|< | + | !align="center"|[[Team:Northwestern/Project|<font color="#000000">'''Project'''</font>]] |
| - | !align="center"|[[Team:Northwestern/SideProject|< | + | !align="center"|[[Team:Northwestern/SideProject|<font color="#000000">'''Human Practices'''</font>]] |
| - | !align="center"|[[Team:Northwestern/Parts|< | + | !align="center"|[[Team:Northwestern/Parts|<font color="#000000">'''Parts'''</font>]] |
| - | !align="center"|[[Team:Northwestern/Notebook|< | + | !align="center"|[[Team:Northwestern/Notebook|<font color="#000000">'''Notebook'''</font>]] |
| - | !align="center"|[[Team:Northwestern/Calendar|< | + | !align="center"|[[Team:Northwestern/Calendar|<font color="#000000">'''Calendar'''</font>]] |
| - | !align="center"|[[Team:Northwestern/Protocol|< | + | !align="center"|[[Team:Northwestern/Protocol|<font color="#000000">'''Protocol'''</font>]] |
| - | !align="center"|[[Team:Northwestern/Safety|< | + | !align="center"|[[Team:Northwestern/Safety|<font color="#000000">'''Safety'''</font>]] |
| - | !align="center"|[[Team:Northwestern/Links|< | + | !align="center"|[[Team:Northwestern/Links|<font color="#000000">'''Links'''</font>]] |
| - | !align="center"|[[Team:Northwestern/References|< | + | !align="center"|[[Team:Northwestern/References|<font color="#000000">'''References'''</font>]] |
| - | !align="center"|[[Team:Northwestern/Media|< | + | !align="center"|[[Team:Northwestern/Media|<font color="#000000">'''Media'''</font>]] |
| - | !align="center"|[[Team:Northwestern/Contact|< | + | !align="center"|[[Team:Northwestern/Contact|<font color="#000000">'''Contact'''</font>]] |
|} | |} | ||
{| align="center" border="0" width="75%" | {| align="center" border="0" width="75%" | ||
| | | | ||
| - | + | =='''Protocols'''== | |
== '''DNA''' == | == '''DNA''' == | ||
[[Team:Northwestern/Protocol/PDNA|Prepping DNA from the kit plates]] | [[Team:Northwestern/Protocol/PDNA|Prepping DNA from the kit plates]] | ||
| - | |||
| - | + | [[Team:Northwestern/Protocol/Quikchange (from primers to colonies!|Quikchange: Primers to Colonies]] | |
| - | + | [[Team:Northwestern/Protocol/Kit to Stock Plasmid|Kit to Stock Plasmid]] | |
| - | + | [[Team:Northwestern/Protocol/Ethanol Precipitation|Ethanol Precipitation]] | |
| - | + | [[Team:Northwestern/Protocol/New Part Design(PCR)|New Part Design]] | |
=='''Bacterial Work'''== | =='''Bacterial Work'''== | ||
| - | + | [[Team:Northwestern/Protocol/Transformation|Transformation]] | |
| - | + | [[Team:Northwestern/Protocol/O/N Culture|Overnight Cultures]] | |
| - | + | [[Team:Northwestern/Protocol/Preparation of Competent Cells|Preparation of Competent Cells]] | |
| - | + | [[Team:Northwestern/Protocol/LB Media|LB Media]] | |
| - | + | [[Team:Northwestern/Protocol/Preparing Plates|Preparing Plates]] | |
| - | + | [[Team:Northwestern/Protocol/Glycerol Stocks|Glycerol Stocks]] | |
=='''Assembly'''== | =='''Assembly'''== | ||
| - | + | [[Team:Northwestern/Protocol/Restriction Enzyme Digests|Restriction Enzyme Digests]] | |
| - | + | ||
| - | + | [[Team:Northwestern/Protocol/Plasmid Construction|Plasmid Construction]] | |
| - | + | ||
| - | + | [[Team:Northwestern/Protocol/3A Assembly|3A Assembly]] | |
| + | |||
| + | [[Team:Northwestern/Protocol/Ligations|Ligations]] | ||
| + | |||
| + | [[Team:Northwestern/Protocol/Mini Prep|Qiagen Mini Prep]] | ||
=='''Microscopy'''== | =='''Microscopy'''== | ||
| - | + | [[Team:Northwestern/Protocol/Confocal Microscopy|Confocal Microscopy]] | |
| - | + | ||
| + | [[Team:Northwestern/Protocol/Fluorescence Microscopy|Fluorescence Microscopy]] | ||
=='''Reagents'''== | =='''Reagents'''== | ||
| - | + | ||
| + | [[Team:Northwestern/Protocol/Reagents|Reagents]] | ||
=='''Cell Staining'''== | =='''Cell Staining'''== | ||
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| + | [[Team:Northwestern/Protocol/Rhodamine-Conjugated Chitin Probe|Rhodamine-Conjugated Chitin Probe]] | ||
| + | [[Team:Northwestern/Protocol/Methanol Fixation|Methanol Fixation]] | ||
| - | + | [[Team:Northwestern/Protocol/LIVE/DEAD® BacLight - Bacterial Viability Kit|Live/Dead Baclight]] | |
|} | |} | ||
Latest revision as of 01:23, 27 October 2010
| Home | Brainstorm | Team | Acknowledgements | Project | Human Practices | Parts | Notebook | Calendar | Protocol | Safety | Links | References | Media | Contact |
|---|
ProtocolsDNAPrepping DNA from the kit plates Quikchange: Primers to Colonies Bacterial WorkPreparation of Competent Cells AssemblyMicroscopyReagentsCell Staining |
