Team:Harvard/allergy/parts

From 2010.igem.org

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<div id="abstract">
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<h1>parts and primers</h1>
 
<h2>Allergy</h2>
<h2>Allergy</h2>
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For more information on the vectors visit our "<a href="allergens">meet the allergens</a>" page<br /><br />
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For more information on the allergens visit our "<a href="allergens">allergen targets</a>" page<br /><br />
<table cellspacing="0">
<table cellspacing="0">
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<tr><td><b>Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Sequence</b></td></tr>
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<tr><td><b>Name</b></td><td><b>Biobrick Name</b></td><td><b>Original Name</b></td><td><b>Description</b></td><td><b>Resistance</b></td><td><b>Sequence</b></td></tr>
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<tr><td>A1</td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td><td><a href="AmirnaGFP.txt"> amirnaGFP</a></td></tr>
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<tr><td>A1</td><td><center>-</center></td><td>amiRNA GFP</td><td>GFP knockdown construct</td><td>chloramphenicol</td><td><a href="AmirnaGFP.txt"> amiRNA GFP</a></td></tr>
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<tr><td>A2</td><td>amiRNA (Other allergen x)</td><td>knockdown construct for x </td><td>chloramphenicol</td><td> <a href="insert">amiRNA x</a></td></tr>
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<tr><td>A2</td><td>BBa_K382017</td><td>amiRNA LTP </td><td>LTP knockdown construct</td><td>chloramphenicol</td><td> <a href="LTP.txt">amiRNA LTP</a></td></tr>
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<tr><td>A3</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>chloramphenicol</td><td><a href="Bet1.txt"> ihpRNA Bet1</a></td></tr>
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<tr><td>A3</td><td>BBa_K382014</td><td>ihpRNA Bet</td><td>Intron hairpin Bet Knockdown construct</td><td>chloramphenicol</td><td><a href="Bet1.txt"> ihpRNA Bet1</a></td></tr>
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<tr><td>A4</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td><a href="LTP.txt">ihpRNA LTP</a></td></tr>
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<tr><td>A4</td><td>BBa_K382015</td><td>ihpRNA LTP</td><td>Intron hairpin LTP Knockdown construct</td><td>chloramphenicol</td><td><a href="LTP.txt">ihpRNA LTP</a></td></tr>
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<tr><td>A5</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td><a href="Ger.txt">ihpRNA GER</a></td></tr>
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<tr><td>A5</td><td>BBa_K382019</td><td>ihpRNA Ger </td><td>Intron hairpin Ger Knockdown construct</td><td>chloramphenicol</td><td><a href="Ger.txt">ihpRNA GER</a></td></tr>
</table>
</table>
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<table cellspacing="0">
<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>
<tr><td><b>Name</b></td><td><b>Description</b></td><td><b>Sequence</b></td></tr>
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<tr><td>VP3</td><td>HindIII-pENTCUP2 forward primer for Expression Series insert</td><td></td></tr>
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<tr><td>AP1</td><td>1 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> GFP primer1</a> </td></tr>
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<tr><td>VP4</td><td>pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert</td><td></td></tr>
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<tr><td>AP2</td><td>2 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> GFP primer2</a></td></tr>
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<tr><td>VP5</td><td>HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>AP3</td><td>3 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> GFP primer3</a></td></tr>
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<tr><td>VP6</td><td>gusA-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>AP4</td><td>4 of 4 primers used to create the amiRNA GFP construct</td><td><a href="AmirnaGFP.txt"> GFP primer4</a></td></tr>
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<tr><td>VP7</td><td>HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>AP5</td><td>1 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt"> Bet1 Primer 1</a></td></tr>
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<tr><td>VP8</td><td>smGFP-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr>
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<tr><td>AP6</td><td>2 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt"> Bet1 Primer 2</a></td></tr>
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<tr><td>VP9</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr>
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<tr><td>AP7</td><td>3 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt"> Bet1 Primer 3</a></td></tr>
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<tr><td>VP11</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr>
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<tr><td>AP8</td><td>4 of 4 primers used to create the amiRNA LTP construct</td><td><a href="LTP.txt"> Bet1 Primer 4</a></td></tr>
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<tr><td>VP12</td><td>1 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>AP9</td><td>Forward primer for Bet v1 Sense</td><td><a href="Bet1.txt"> Bet1 Sense Forward</a></td></tr>
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<tr><td>VP13</td><td>2 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>AP10</td><td>Reverse primer for Bet v1 Sense </td><td><a href="Bet1.txt"> Bet1 Sense Reverse</a></td></tr>
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<tr><td>VP14</td><td>3 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>AP11</td><td>Forward primer for Bet v1 Antisense</td><td><a href="Bet1.txt">Bet1 Antisense Forward</a></td></tr>
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<tr><td>VP15</td><td>4 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>AP12</td><td>Reverse primer for Bet v1 Antisense</td><td><a href="Bet1.txt"> Bet1 Antisense Reverse</a></td></tr>
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<tr><td>VP16</td><td>5 of 5 for sequencing V12 biobrick cloning site and smgfp reporter</td><td></td></tr>
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<tr><td>AP13</td><td>Forward primer for LTP Sense</td><td><a href="LTP.txt"> LTP Sense Forward</a></td></tr>
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<tr><td>VP17</td><td>1 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP14</td><td>Reverse primer for LTP Sense</td><td><a href="LTP.txt">LTP Sense Reverse</a></td></tr>
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<tr><td>VP18</td><td>2 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP15</td><td>Forward primer for LTP Antisense</td><td><a href="LTP.txt">LTP Antisense Forward</a></td></tr>
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<tr><td>VP19</td><td>3 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP16</td><td>Reverse primer for LTP Antisense</td><td><a href="LTP.txt">LTP Antisense Reverse</a></td></tr>
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<tr><td>VP20</td><td>4 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP17</td><td>Forward primer for Ger Sense</td><td><a href="Ger.txt">Ger Sense Forward</a></td></tr>
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<tr><td>VP21</td><td>5 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP18</td><td>Reverse primer for Ger Sense</td><td><a href="Ger.txt">Ger Sense Reverse</a></td></tr>
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<tr><td>VP22</td><td>6 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP19</td><td>Forward primer for Ger Antisense</td><td><a href="Ger.txt">Ger Antisense Forward</a></td></tr>
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<tr><td>VP23</td><td>7 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP20</td><td>Reverse primer for Ger Antisense</td><td><a href="Ger.txt">Ger Antisense Reverse</a></td></tr>
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<tr><td>VP24</td><td>8 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP21</td><td>Forward Primer for PDK intron</td><td><a href="Introns"> PDK forward</a></td></tr>
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<tr><td>VP25</td><td>9 of 9 for sequencing V11 biobrick cloning site and gusA reporter</td><td></td></tr>
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<tr><td>AP22</td><td>Reverse Primer for PDK intron</td><td><a href="Introns"> PDK reverse</a></td></tr>
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<tr><td>VP26</td><td>1 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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        <tr><td>AP23</td><td>Forward Primer to make amiRNA Biobrick compatible</td><td><a href="amiRNA"> Biobrick forward</a></td></tr>
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<tr><td>VP27</td><td>2 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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        <tr><td>AP24</td><td>Reverse Primer to make amiRNA Biobrick compatible</td><td><a href="amiRNA"> Biobrick reverse</a></td></tr>
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<tr><td>VP28</td><td>3 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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        <tr><td>AP25</td><td>1 of 4 primers used to create the amiRNA Bet construct</td><td><a href="Bet1.txt">LTP primer 1</a></td></tr>
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<tr><td>VP29</td><td>4 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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        <tr><td>AP26</td><td>2 of 4 primers used to create the amiRNA Bet construct</td><td><a href="Bet1.txt">LTP primer 2</a></td></tr>
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<tr><td>VP30</td><td>5 of 5 for sequencing V9 and V10 biobrick cloning site and promoter</td><td></td></tr>
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        <tr><td>AP27</td><td>3 of 4 primers used to create the amiRNA Bet construct</td><td><a href="Bet1.txt">LTP primer 3</a></td></tr>
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<tr><td>VP33</td><td>1 of 2 for sequencing V7 and V8 biobrick cloning site</td><td></td></tr>
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        <tr><td>AP28</td><td>4 of 4 primers used to create the amiRNA Bet construct</td><td><a href="Bet1.txt">LTP primer 4</a></td></tr>
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<tr><td>VP34</td><td>2 of 2 for sequencing V7 and V8 biobrick cloning site</td><td></td></tr>
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Latest revision as of 00:04, 27 October 2010



parts and primers

Allergy

For more information on the allergens visit our "allergen targets" page

NameBiobrick NameOriginal NameDescriptionResistanceSequence
A1
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amiRNA GFPGFP knockdown constructchloramphenicol amiRNA GFP
A2BBa_K382017amiRNA LTP LTP knockdown constructchloramphenicol amiRNA LTP
A3BBa_K382014ihpRNA BetIntron hairpin Bet Knockdown constructchloramphenicol ihpRNA Bet1
A4BBa_K382015ihpRNA LTPIntron hairpin LTP Knockdown constructchloramphenicolihpRNA LTP
A5BBa_K382019ihpRNA Ger Intron hairpin Ger Knockdown constructchloramphenicolihpRNA GER


Primers

NameDescriptionSequence
AP11 of 4 primers used to create the amiRNA GFP construct GFP primer1
AP22 of 4 primers used to create the amiRNA GFP construct GFP primer2
AP33 of 4 primers used to create the amiRNA GFP construct GFP primer3
AP44 of 4 primers used to create the amiRNA GFP construct GFP primer4
AP51 of 4 primers used to create the amiRNA LTP construct Bet1 Primer 1
AP62 of 4 primers used to create the amiRNA LTP construct Bet1 Primer 2
AP73 of 4 primers used to create the amiRNA LTP construct Bet1 Primer 3
AP84 of 4 primers used to create the amiRNA LTP construct Bet1 Primer 4
AP9Forward primer for Bet v1 Sense Bet1 Sense Forward
AP10Reverse primer for Bet v1 Sense Bet1 Sense Reverse
AP11Forward primer for Bet v1 AntisenseBet1 Antisense Forward
AP12Reverse primer for Bet v1 Antisense Bet1 Antisense Reverse
AP13Forward primer for LTP Sense LTP Sense Forward
AP14Reverse primer for LTP SenseLTP Sense Reverse
AP15Forward primer for LTP AntisenseLTP Antisense Forward
AP16Reverse primer for LTP AntisenseLTP Antisense Reverse
AP17Forward primer for Ger SenseGer Sense Forward
AP18Reverse primer for Ger SenseGer Sense Reverse
AP19Forward primer for Ger AntisenseGer Antisense Forward
AP20Reverse primer for Ger AntisenseGer Antisense Reverse
AP21Forward Primer for PDK intron PDK forward
AP22Reverse Primer for PDK intron PDK reverse
AP23Forward Primer to make amiRNA Biobrick compatible Biobrick forward
AP24Reverse Primer to make amiRNA Biobrick compatible Biobrick reverse
AP251 of 4 primers used to create the amiRNA Bet constructLTP primer 1
AP262 of 4 primers used to create the amiRNA Bet constructLTP primer 2
AP273 of 4 primers used to create the amiRNA Bet constructLTP primer 3
AP284 of 4 primers used to create the amiRNA Bet constructLTP primer 4

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