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Team:Michigan/Protocols
From 2010.igem.org
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*Twice as efficient as standard heat shock | *Twice as efficient as standard heat shock | ||
| - | + | [[Media:Protocol_Heat_Shock_Transformation_2.pdf|Transformation--heat shock]] | |
| - | [[Media:Protocol_Heat_Shock_Transformation_2.pdf|Transformation-heat shock]] | + | |
*Getting parts from the 360 well registry plates | *Getting parts from the 360 well registry plates | ||
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*Heat shock | *Heat shock | ||
| - | [[Media:General_Transformation_Protocol.pdf|Transformation-electroporation]] | + | [[Media:General_Transformation_Protocol.pdf|Transformation--electroporation]] |
*Getting parts from the 360 well registry plates | *Getting parts from the 360 well registry plates | ||
*Competent cell preparation | *Competent cell preparation | ||
Revision as of 23:58, 26 October 2010
Protocols
Using Lab Equipment
Obtaining Deionized Water in the ERB
Enigeering Research Building Autoclave
Epifluorescence Microscope Usage - H.H. Dow
Cell Culture
Making cultures from a -80C freezer stock
P. putida KT2440 antibotic resistance tolerance
Competent Cell Preparation for Quick Transformation of E. coli
DNA Manipulation
Direct Plating Transformation Protocol
- Very simple protocol
- Twice as efficient as standard heat shock
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Heat shock
Transformation--electroporation
- Getting parts from the 360 well registry plates
- Competent cell preparation
- Electroporation
- Adopted from Jeremy Minty's protocol
- General protocol with specific instructions for ERB/Lin lab equipment
- Efficient protocol for screening transformants after a ligation
Updated T4 DNA Ligase Protocol (Not quick ligase)
- For additional information/questions, refer to [http://www.neb.com/nebecomm/tech_reference/modifying_enzymes/ligation_tips.asp NEB Ligation Tips]
- Ligation
- Ginko Bioworks Protocol
- Jeremy Minty Protocol
Group-Specific Protocols
Oil Sands
Revised biofilm assay protocol
- Updated Colony PCR Protocol
Pili
Quorum Sensing
General Outline for Biobrick Manipulations
