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Revision as of 02:50, 7 July 2010 (view source) 19symon (Talk | contribs) m (→Making the Inducible T7 Expression Vector) ← Older edit		Revision as of 05:38, 7 July 2010 (view source) SebastianR (Talk | contribs) (→Making the Inducible T7 Expression Vector) Newer edit →
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-	=== Making the Inducible T7 Expression Vector ===	+	=== Making Vector ===
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-	The aim for this section is to create an inducible expression backbone. This will be achieved by altering the backbone biobrick '''BBa_J04500''', by removing the -35 and -10 elements (promoter) and inserting the bacteriophage T7 promoter. The strategy used to will be Quikchange XL mutagenesis to alter the promoter elements into restriction sites:	+
-	* -35 --> AflII	+
-	* -10 --> BamH1	+
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-	These restriction sites are NOT found in the BBa_J04500 part or vector (pSB1A2), so there will be no cross cutting.	+
-		+
-	The T7 promoter is small enough for it to be constructed from primers. Two ligation ends would be designed to match the sticky ends of the restriction sites introduced. The T7 can thus be inserted as the promoter of the system, being regulated by IPTG (LacO site) and glucose/cAMP (CAP-binding site).	+
	== Results ==		== Results ==

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Revision as of 05:38, 7 July 2010

Overall project

Your abstract

Project Details

Creation of the Taq Polymerase Part

Making Vector

Results