Team:IvyTech-South Bend/Parts

From 2010.igem.org

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The T9002 AHL detector was ligated to a LacZ reporter gene by the conventional biobrick assembly #10.
The T9002 AHL detector was ligated to a LacZ reporter gene by the conventional biobrick assembly #10.
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https://static.igem.org/mediawiki/2010/5/59/IvyTechSBend-BBa_K498000.png
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Sequence and Features
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Format: Subparts|Ruler|SS|DSSearch: Length: 3154 bpContext: Part onlyGet selected sequence
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. .1 11 21 31 41 51 61 71 81 91
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1 500 1K 1.5K 2K 2.5K 3K 3.5K 4K
+
-
T9002
+
-
I732094
+
-
E0044
+
-
C0062
+
-
E0040
+
-
I732006
+
-
B0010
+
-
B0010
+
-
B0010
+
-
R0062
+
-
R0040
+
-
B0012
+
-
B0012
+
-
B0012
+
-
I732007
+
-
B0032
+
-
B0034
+
-
B0034
+
-
B0034
+
-
prefix
+
-
luxR
+
-
lacZ
+
-
STOP
+
-
Stop
+
-
Stop
+
-
Stop
+
-
-35
+
-
-10
+
-
-35
+
-
-10
+
-
RBS-3Weak
+
-
RBS
+
-
LuxR/HSL
+
-
TetR 1
+
-
TetR 2
+
-
T7 TE
+
-
T7 TE
+
-
T7 TE
+
-
BglII
+
-
BamHI
+
-
PolyA
+
-
PolyA
+
-
PolyA
+
-
T
+
-
A
+
-
START
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-
tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag agaaagagga gaaatactag atgaaaaaca taaatgccga
+
-
1
+
-
agggatagtc actatctcta actgtaggga tagtcactat ctctatgact cgtgatgatc tctttctcct ctttatgatc tactttttgt atttacggct
+
-
T9002
+
-
C0062
+
-
R0040
+
-
B0034
+
-
prefix
+
-
luxR
+
-
-35
+
-
-10
+
-
TetR 1
+
-
TetR 2
+
-
cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat
+
-
101
+
-
gctgtgtatg tcttattaat tattttaatt tcgaacatct tcgttattac tataattagt tacgaataga ctatactgat tttaccatgt aacacttata
+
-
T9002
+
-
C0062
+
-
luxR
+
-
tatttactcg cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
+
-
201
+
-
ataaatgagc gctagtaaat aggagtaaga taccaattta gactataaag ttaggatcta ttaatgggat tttttacctc cgttataata ctactgcgat
+
-
T9002
+
-
C0062
+
-
luxR
+
-
T
+
-
atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc
+
-
301
+
-
taaattattt tatactagga tatcatctaa taagattgag gttagtaagt ggttaattaa ccttatataa acttttgtta cgacatttat tttttagagg
+
-
T9002
+
-
C0062
+
-
luxR
+
-
aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat
+
-
401
+
-
tttacattaa tttcttcgct tttgtagtcc agaatagtga cccaaatcaa agggataagt atgccgattg ttaccgaagc cttacgaatc aaaacgtgta
+
-
T9002
+
-
C0062
+
-
luxR
+
-
tcagaaaaag acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
+
-
501
+
-
agtctttttc tgttgatata tctatcaaat aaaaatgtac gcacatactt gtatggtaat taacaaggaa gagatcaact attaatagct ttttatttat
+
-
T9002
+
-
C0062
+
-
luxR
+
-
A
+
-
tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt caaaaatatt
+
-
601
+
-
atcgtttatt atttagtttg ttgctaaatt ggttttctct ttttcttaca aatcgcaccc gtacgcttcc tttttcgaga accctataaa gtttttataa
+
-
T9002
+
-
C0062
+
-
luxR
+
-
aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca
+
-
701
+
-
tccaacgtca ctcgcatgac agtgaaaggt aaattggtta cgcgtttact ttgagttatg ttgtttggcg acggtttcat aaagatttcg ttaaaattgt
+
-
T9002
+
-
C0062
+
-
luxR
+
-
ggagcaattg attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
+
-
801
+
-
cctcgttaac taacgggtat gaaattttta attattgtga ctatcacgat cacatctagt gatgatctcg gtccgtagtt tattttgctt tccgagtcag
+
-
T9002
+
-
C0062
+
-
B0010
+
-
luxR
+
-
gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
+
-
901
+
-
ctttctgacc cggaaagcaa aatagacaac aaacagccac ttgcgagaga tgatctcagt gtgaccgagt ggaagcccac ccggaaagac gcaaatatat
+
-
T9002
+
-
B0010
+
-
B0012
+
-
Stop
+
-
T7 TE
+
-
PolyA
+
-
ctagagacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagagt cacacaggaa agtactagat gcgtaaagga
+
-
1001
+
-
gatctctgga catcctagca tgtccaaatg cgttctttta ccaaacaata tcagcttatt tatgatctca gtgtgtcctt tcatgatcta cgcatttcct
+
-
T9002
+
-
E0040
+
-
R0062
+
-
B0032
+
-
-35
+
-
-10
+
-
RBS-3Weak
+
-
RBS
+
-
LuxR/HSL
+
-
gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa
+
-
1101
+
-
cttcttgaaa agtgacctca acagggttaa gaacaactta atctaccact acaattaccc gtgtttaaaa gacagtcacc tctcccactt ccactacgtt
+
-
T9002
+
-
E0040
+
-
catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt
+
-
1201
+
-
gtatgccttt tgaatgggaa tttaaataaa cgtgatgacc ttttgatgga caaggtaccg gttgtgaaca gtgatgaaag ccaataccac aagttacgaa
+
-
T9002
+
-
E0040
+
-
tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
+
-
1301
+
-
acgctctatg ggtctagtat actttgtcgt actgaaaaag ttctcacggt acgggcttcc aatacatgtc ctttcttgat ataaaaagtt tctactgccc
+
-
T9002
+
-
E0040
+
-
aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg
+
-
1401
+
-
ttgatgttct gtgcacgact tcagttcaaa cttccactat gggaacaatt atcttagctc aattttccat aactaaaatt tcttctacct ttgtaagaac
+
-
T9002
+
-
E0040
+
-
gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat
+
-
1501
+
-
ctgtgtttaa ccttatgttg atattgagtg tgttacatat gtagtaccgt ctgtttgttt tcttacctta gtttcaattg aagttttaat ctgtgttgta
+
-
T9002
+
-
E0040
+
-
tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
+
-
1601
+
-
acttctacct tcgcaagttg atcgtctggt aatagttgtt ttatgaggtt aaccgctacc gggacaggaa aatggtctgt tggtaatgga caggtgtgtt
+
-
T9002
+
-
E0040
+
-
tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca
+
-
1701
+
-
agacgggaaa gctttctagg gttgcttttc tctctggtgt accaggaaga actcaaacat tgtcgacgac cctaatgtgt accgtaccta cttgatatgt
+
-
T9002
+
-
E0040
+
-
aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
+
-
1801
+
-
ttattattat gatctcggtc cgtagtttat tttgctttcc gagtcagctt tctgacccgg aaagcaaaat agacaacaaa cagccacttg cgagagatga
+
-
T9002
+
-
E0040
+
-
B0010
+
-
agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gaggaagatc ttccggatcc ggtactagag aaagaggaga aatactagat
+
-
1901
+
-
tctcagtgtg accgagtgga agcccacccg gaaagacgca aatatatgat ctccttctag aaggcctagg ccatgatctc tttctcctct ttatgatcta
+
-
T9002
+
-
I732094
+
-
I732006
+
-
B0012
+
-
I732007
+
-
B0034
+
-
lacZ
+
-
Stop
+
-
T7 TE
+
-
BglII
+
-
BamHI
+
-
PolyA
+
-
START
+
-
gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
+
-
2001
+
-
ctggtactaa tgcctaagtg accggcagca aaatgttgca gcactgaccc ttttgggacc gcaatgggtt gaattagcgg aacgtcgtgt agggggaaag
+
-
I732094
+
-
I732006
+
-
lacZ
+
-
START
+
-
gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag
+
-
2101
+
-
cggtcgaccg cattatcgct tctccgggcg tggctagcgg gaagggttgt caacgcgtcg gacttaccgc ttaccgcgaa acggaccaaa ggccgtggtc
+
-
I732094
+
-
I732006
+
-
lacZ
+
-
aagcggtgcc ggaaagctgg ctggagtaat aatactagag aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
+
-
2201
+
-
ttcgccacgg cctttcgacc gacctcatta ttatgatctc tttctcctct ttatgatcta cgcatttcct cttcttgaaa agtgacctca acagggttaa
+
-
I732094
+
-
E0044
+
-
I732006
+
-
B0034
+
-
lacZ
+
-
STOP
+
-
cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt
+
-
2301
+
-
gaacaactta atctaccact acaattaccc gtgtttaaaa gacagtcacc tctcccactt ccactacgtt gtatgccttt tgaatgggaa tttaaataaa
+
-
I732094
+
-
E0044
+
-
gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca
+
-
2401
+
-
cgtgatgacc ttttgatgga caaggtaccg gttgtgaaca gtgatgaaag ccaataccac aagttacgaa acgctctatg ggtctagtat actttgtcgt
+
-
I732094
+
-
E0044
+
-
tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
+
-
2501
+
-
actgaaaaag ttctcacggt acgggcttcc aatacatgtc ctttcttgat ataaaaagtt tctactgccc ttgatgttct gtgcacgact tcagttcaaa
+
-
I732094
+
-
E0044
+
-
gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac
+
-
2601
+
-
cttccactat gggaacaatt atcttagctc aattttccat aactaaaatt tcttctacct ttgtaagaac ctgtgtttaa ccttatgttg atattgagtg
+
-
I732094
+
-
E0044
+
-
acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
+
-
2701
+
-
tgttacatat gtagtaccgt ctgtttgttt tcttacctta gtttcaattg aagttttaat ctgtgttgta acttctacct tcgcaagttg atcgtctggt
+
-
I732094
+
-
E0044
+
-
ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
+
-
2801
+
-
aatagttgtt ttatgaggtt aaccgctacc gggacaggaa aatggtctgt tggtaatgga caggtgtgtt agacgggaaa gctttctagg gttgcttttc
+
-
I732094
+
-
E0044
+
-
agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aaaggcctgc tgcaaacgac gaaaactacg
+
-
2901
+
-
tctctggtgt accaggaaga actcaaacat tgtcgacgac cctaatgtgt accgtaccta cttgatatgt tttccggacg acgtttgctg cttttgatgc
+
-
I732094
+
-
E0044
+
-
ctgctgctgt ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
+
-
3001
+
-
gacgacgaca aattattatg atctcggtcc gtagtttatt ttgctttccg agtcagcttt ctgacccgga aagcaaaata gacaacaaac agccacttgc
+
-
I732094
+
-
E0044
+
-
B0010
+
-
ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
+
-
3101
+
-
gagagatgat ctcagtgtga ccgagtggaa gcccacccgg aaagacgcaa atat
+
-
I732094
+
-
B0010
+
-
B0012
+
-
Stop
+
-
T7 TE
+
-
PolyA
+
-
 
+
-
p(tetR)
+
-
 
+
-
R0040
+
-
 
+
-
.
+
-
 
+
-
B0034
+
-
 
+
-
luxr
+
-
 
+
-
C0062
+
-
 
+
-
.
+
-
 
+
-
B0010
+
-
 
+
-
.
+
-
 
+
-
B0012
+
-
 
+
-
lux pR
+
-
 
+
-
R0062
+
-
 
+
-
.
+
-
 
+
-
B0032
+
-
 
+
-
GFP
+
-
 
+
-
E0040
+
-
 
+
-
.
+
-
 
+
-
B0010
+
-
 
+
-
.
+
-
 
+
-
B0012
+
-
 
+
-
Adapter
+
-
 
+
-
I732007
+
-
 
+
-
.
+
-
 
+
-
B0034
+
-
 
+
-
lacZ-alpha
+
-
 
+
-
I732006
+
-
 
+
-
.
+
-
 
+
-
B0034
+
-
 
+
-
.
+
-
 
+
-
E0044
+
-
 
+
-
.
+
-
 
+
-
B0010
+
-
 
+
-
.
+
-
 
+
-
B0012
+
Assembly Compatibility:
Assembly Compatibility:
Line 409: Line 77:
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
+
 
|[[Image:IvyTech-South_Bend_team.png|right|frame|Your team picture]]
|[[Image:IvyTech-South_Bend_team.png|right|frame|Your team picture]]
|-
|-

Latest revision as of 19:39, 26 October 2010



Part:BBa_K498000

Designed by IvyTech_South-Bend Team Group: iGEM10_IvyTechSBend-IN-USA (2010-10-26) From partsregistry.org


T9002 - LacZ

The T9002 AHL detector was ligated to a LacZ reporter gene by the conventional biobrick assembly #10. IvyTechSBend-BBa_K498000.png

Assembly Compatibility: 

   * 10
     COMPATIBLE WITH RFC[10]
   * 12
     COMPATIBLE WITH RFC[12]
   * 21
     INCOMPATIBLE WITH RFC[21]
     Illegal BglII site found at 1956
     Illegal BamHI site found at 1965
   * 23
     COMPATIBLE WITH RFC[23]
   * 25
     COMPATIBLE WITH RFC[25]


IvyTech-South Bend logo.png
Your team picture
Team Example




Parts

New for iGEM 2010 is the groupparts tag. This tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.

<groupparts>iGEM010 IvyTech-South_Bend</groupparts>

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety
Retrieved from "http://2010.igem.org/Team:IvyTech-South_Bend/Parts"